| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg | |||
| Other Sizes |
| Targets |
Green DND-26 targets lysosomes, the acidic organelles responsible for cellular degradation and recycling. The probe does not have a traditional pharmacological target but rather functions as a fluorescent marker for lysosomes. The weak base moiety of the dye is protonated in the acidic environment of lysosomes (pH ~4.5-5.0), causing the dye to become trapped within the organelle. The fluorescein group provides green fluorescence that allows for visualization and quantification of lysosomal content and dynamics in living cells.
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| ln Vitro |
1. Working solution configuration: 1.1 Centrifuge the solution right away to concentrate at the tube's bottom and return it to a constant temperature. 1.2 To get the working concentration of the 1 mM storage solution, dilute it with culture medium or an appropriate buffer (like PBS). Note on recommended working solution concentration: Please make sure that the Green DND-26 working solution is ready for usage by adjusting its concentration to match the current circumstances. 2. 2.1 Suspension cells for cell staining: Centrifuge cells, add PBS, and wash twice for five minutes each time. Adherent cells: remove cells and add digested islets of cell material. Add PBS and centrifuge, then discard the supernatant. Wash twice, for five minutes each time. 2.2 After adding 1 milliliter of the Green DND-26 working solution, incubate for half an hour. 2.3 Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. 2.4 Wash the cells twice with PBS, giving them five minutes each time. 2.5 Re-suspend the cells in 1 milliliter of PBS or serum-free water, and examine them using a flow cytometer or fluorescence microscope. Storage temperature: -20°C, shielded from light
In vitro, Green DND-26 is used for live-cell imaging and flow cytometry to label and visualize lysosomes. It is suitable for observing lysosomal internal biosynthesis and related pathogenesis. The dye can be used to study lysosomal trafficking, acidification, and function in various cell types. It is commonly used in combination with other fluorescent probes for multicolor imaging experiments. The recommended working concentration is 50-100 nM. |
| ln Vivo |
In vivo, Green DND-26 is primarily used for ex vivo applications rather than direct in vivo administration. It can be used to label lysosomes in cells isolated from tissues or in tissue sections for histological analysis. The dye has been used to study lysosomal dynamics in zebrafish and other model organisms. However, its use in live mammals is limited due to potential toxicity and rapid clearance. Its primary applications are in vitro for cell biology research.
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| Enzyme Assay |
For in vitro fluorescence characterization, Green DND-26 is dissolved in appropriate buffer, and its absorption and emission spectra are measured using UV-visible and fluorescence spectrophotometers. The maximum excitation is at 504 nm and emission at 511 nm. The pH-dependent fluorescence properties can be characterized by measuring fluorescence intensity in buffers of varying pH to confirm the lysosomal targeting mechanism.
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| Cell Assay |
For live cell staining, cells are cultured on coverslips or in imaging dishes. The Green DND-26 stock solution (1 mM) is diluted to a working concentration of 50-100 nM in pre-warmed culture medium or appropriate buffer. Cells are incubated with the working solution for 30 minutes at room temperature. Cells are then washed twice with PBS for 5 minutes each. Labeled cells are imaged using fluorescence microscopy with FITC filter sets (excitation ~504 nm, emission ~511 nm) or analyzed by flow cytometry.
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| Animal Protocol |
For in vivo applications, Green DND-26 is not typically administered systemically to mammals. In zebrafish or other small model organisms, the dye can be added to the water or injected into embryos for live imaging of lysosomal dynamics. Cells isolated from tissues can be stained ex vivo with Green DND-26 following the cell staining protocol described above, then analyzed by flow cytometry or microscopy. Tissue sections can also be stained with the dye for histological analysis of lysosomal distribution.
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| ADME/Pharmacokinetics |
Green DND-26 is soluble in DMSO (125 mg/mL, 345.08 mM). The compound should be protected from light during handling and storage to prevent photobleaching. Storage is recommended at 4°C for long-term stability, and at -80°C for 6 months or -20°C for 1 month in solvent. The dye is for research use only and is not intended for human or veterinary use.
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| Toxicity/Toxicokinetics |
Toxicological data for Green DND-26 have not been extensively reported, as it is a fluorescent probe rather than a therapeutic agent. At the low concentrations used for cell staining (50-100 nM), the dye is generally well-tolerated by cells for short-term experiments. Higher concentrations or prolonged exposure may be cytotoxic. The compound is for research use only and is not intended for human use. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
DND-26 dye is a BODIPY dye that is used as a fluorescent dye.
Green DND-26 (LysoTracker Green DND-26) is a green fluorescent lysosomal probe with excitation/emission at 504/511 nm. It consists of a fluorescein group linked to weak bases that accumulate in lysosomes. The dye is used for live-cell imaging of lysosomal dynamics, biosynthesis, and pathogenesis. It is suitable for fluorescence microscopy and flow cytometry applications. Green DND-26 is a research tool for cell biology and is not approved for clinical use. |
| Molecular Formula |
C18H25BN4OF2
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|---|---|
| Molecular Weight |
362.23
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| Exact Mass |
362.208
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| CAS # |
220524-71-0
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| PubChem CID |
15410444
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| Appearance |
Orange to red solid powder
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| Melting Point |
120 °C
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
26
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| Complexity |
674
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
SXZBATLAPYSXND-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H25BF2N4O/c1-13-11-14(2)24-17(13)12-16-6-5-15(25(16)19(24,20)21)7-8-18(26)22-9-10-23(3)4/h5-6,11-12H,7-10H2,1-4H3,(H,22,26)
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| Chemical Name |
3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)-N-[2-(dimethylamino)ethyl]propanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7607 mL | 13.8034 mL | 27.6068 mL | |
| 5 mM | 0.5521 mL | 2.7607 mL | 5.5214 mL | |
| 10 mM | 0.2761 mL | 1.3803 mL | 2.7607 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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