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| Other Sizes |
| Targets |
H-Leu-Gly-OH does not have a specific biological target as a drug. It is a metabolite and a zwitterionic tautomer. The leucine residue provides hydrophobic properties, while glycine adds flexibility to the peptide structure. The compound may be recognized by certain peptidases, but it is not designed to interact with specific receptors or enzymes for therapeutic purposes. Its primary utility is as a chemical building block and research tool for studying peptide chemistry and metabolism.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro, H-Leu-Gly-OH is used as a substrate in enzymatic assays to study the activity of peptidases and proteases. It is also used in peptide synthesis and biochemical research to study protein interactions and peptide bonding. The compound does not exhibit pharmacological activities such as receptor binding or enzyme inhibition. Its role in research is as a reagent for studying peptide chemistry, metabolism, and the role of small peptides in biological systems. |
| ln Vivo |
H-Leu-Gly-OH is not a pharmacologically active compound and therefore does not have defined in vivo activity as a therapeutic agent. It is a metabolite that has been reported to exist in Saccharomyces cerevisiae. When administered to animals, it would likely be metabolized by peptidases to release leucine and glycine, which would then enter normal metabolic pathways. Its primary value remains in biochemical research, where it serves as a tool for studying peptide metabolism and the role of small peptides in biological systems.
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| Enzyme Assay |
In vitro enzyme assays for H-Leu-Gly-OH are typically designed to study peptidase or protease activity. A standard protocol involves incubating the compound with an enzyme preparation in a suitable buffer at physiological pH and temperature. The hydrolysis of the dipeptide bond releases leucine and glycine, which can be quantified by HPLC or mass spectrometry. The progress of the reaction can be monitored, and kinetic parameters such as Km and Vmax can be determined. These assays are used to characterize the substrate specificity of peptidases.
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| Cell Assay |
In vitro cellular assays using H-Leu-Gly-OH are limited because the compound is primarily a research reagent. However, it can be used in cell culture studies to investigate peptide transport and metabolism. Cells are cultured in media supplemented with the compound, and its uptake and hydrolysis are monitored. The effects of increased leucine and glycine availability on cellular metabolism can be assessed. These experiments are typically conducted in cell lines that express peptide transporters, such as Caco-2 cells.
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| Animal Protocol |
In vivo animal studies with H-Leu-Gly-OH are not typically conducted for therapeutic purposes, as it is a research tool. However, it may be used in nutritional or metabolic studies to investigate peptide absorption and metabolism. A typical protocol involves oral or intravenous administration of the compound to rodents. Blood and tissue samples are collected to measure leucine and glycine levels, allowing assessment of the compound's bioavailability and metabolic fate. These studies help to understand the digestion and absorption of small peptides.
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| ADME/Pharmacokinetics |
As a small, hydrophilic dipeptide, H-Leu-Gly-OH is expected to be rapidly absorbed and metabolized after oral administration. It is likely hydrolyzed by peptidases in the gastrointestinal tract and plasma to release leucine and glycine, which then enter the endogenous amino acid pool. The compound's pharmacokinetic properties are characteristic of small peptides, with rapid clearance and short half-life. Detailed pharmacokinetic data are not typically reported, as the compound is used primarily as a research reagent.
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| Toxicity/Toxicokinetics |
H-Leu-Gly-OH is generally considered to have low toxicity, consistent with its composition of the endogenous amino acids leucine and glycine. Acute toxicity is expected to be minimal. The compound may cause mild irritation upon contact with skin, eyes, or mucous membranes. It is not classified as a carcinogen, mutagen, or reproductive toxicant. Standard laboratory safety precautions, including the use of personal protective equipment, are recommended. No significant systemic toxicity is anticipated at typical research doses.
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| References | |
| Additional Infomation |
Leu-Gly is a dipeptide composed of L-leucine and glycine residues. It is a metabolite and a zwitterionic tautomer of Leu-Gly. Leu-Gly has been reported to exist in Saccharomyces cerevisiae, and relevant data are available for reference.
H-Leu-Gly-OH (L-Leucyl-glycine, CAS 686-50-0) is a dipeptide composed of L-leucine and glycine. Its molecular formula is C₈H₁₆N₂O₃ and its molecular weight is 188.23 g/mol. It is a metabolite that has been reported to exist in yeast. The leucine residue provides hydrophobic properties, while glycine adds flexibility to the peptide structure. It is used in peptide synthesis and biochemical research to study protein interactions, enzymatic activity, and peptide bonding. |
| Molecular Formula |
C8H16N2O3
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|---|---|
| Molecular Weight |
188.2242
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| Exact Mass |
188.116
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| CAS # |
686-50-0
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| PubChem CID |
97364
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| Appearance |
White to light yellow solid powder
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| Melting Point |
-245ºC (dec.)
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| Index of Refraction |
86 ° (C=2, H2O)
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| LogP |
0.651
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
13
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| Complexity |
192
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(C)C[C@@H](C(=O)NCC(=O)O)N
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| InChi Key |
LESXFEZIFXFIQR-LURJTMIESA-N
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| InChi Code |
InChI=1S/C8H16N2O3/c1-5(2)3-6(9)8(13)10-4-7(11)12/h5-6H,3-4,9H2,1-2H3,(H,10,13)(H,11,12)/t6-/m0/s1
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| Chemical Name |
2-[[(2S)-2-amino-4-methylpentanoyl]amino]acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.3129 mL | 26.5647 mL | 53.1293 mL | |
| 5 mM | 1.0626 mL | 5.3129 mL | 10.6259 mL | |
| 10 mM | 0.5313 mL | 2.6565 mL | 5.3129 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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