| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
Gly-AMC targets aminopeptidases and deubiquitinating enzymes (DUBs). As a substrate for aminopeptidase, Gly-AMC is cleaved by these enzymes to release the fluorescent product 7-amino-4-methylcoumarin (AMC). The fluorescence can be measured to quantify enzyme activity. Gly-AMC is also used to make the C-terminal conjugate of ubiquitin with 7-amino-4-methylcoumarin (Ub-AMC), which is an important probe for fluorescence-based analysis of deubiquitinating enzyme (DUB) activity. DUBs are enzymes that remove ubiquitin from proteins, and their activity is critical for regulating protein degradation, cell signaling, and other cellular processes. Gly-AMC is a valuable tool for studying these enzymes and their role in various biological processes.
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| ln Vitro |
In vitro studies have demonstrated that Gly-AMC is a substrate for aminopeptidase, releasing the fluorescent product 7-amino-4-methylcoumarin (AMC) upon cleavage. The compound is also used to make the C-terminal conjugate of ubiquitin with 7-amino-4-methylcoumarin (Ub-AMC), which is a substrate for deubiquitinating enzymes (DUBs). These in vitro applications make Gly-AMC a valuable tool for studying enzyme specificity, inhibitor screening, and metabolic processes involving peptide cleavage. The compound's fluorescence-based detection allows for sensitive and quantitative measurement of enzyme activity. Gly-AMC is widely used in biochemical assays to characterize the activity of aminopeptidases and DUBs.
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| ln Vivo |
In vivo studies on Gly-AMC are not typically performed, as the compound is primarily used as a research tool in biochemical assays rather than as a therapeutic agent. The compound's use is limited to in vitro applications, such as enzyme activity assays and inhibitor screening. Gly-AMC is not intended for in vivo use or for therapeutic applications. The compound is a valuable tool for studying enzyme function and regulation in vitro. Further studies may explore the use of Gly-AMC in more complex biological systems, but these are not extensively documented.
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| Enzyme Assay |
Non-cellular enzyme assays for Gly-AMC typically involve measuring the activity of aminopeptidase or deubiquitinating enzymes (DUBs) using Gly-AMC as a substrate. The assay uses purified enzyme and Gly-AMC in a buffer system. The enzyme cleaves Gly-AMC to release the fluorescent product 7-amino-4-methylcoumarin (AMC), which is measured using a fluorometer with excitation at ~360 nm and emission at ~460 nm. Gly-AMC is tested at various concentrations to determine the kinetic parameters of the enzyme, such as Km and Vmax. These assays are essential for characterizing the activity and specificity of aminopeptidases and DUBs and for screening for inhibitors of these enzymes.
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| Cell Assay |
In vitro cell-based assays for Gly-AMC are not typically performed, as the compound is primarily used as a substrate in biochemical assays rather than as a bioactive compound in cell-based systems. However, cell lysates or cell extracts may be used as a source of enzymes in assays with Gly-AMC to measure endogenous aminopeptidase or DUB activity. These assays typically involve incubating cell lysates with Gly-AMC and measuring the release of fluorescent AMC. The assays can be used to compare enzyme activity between different cell types or under different conditions. Gly-AMC is typically dissolved in DMSO or water and diluted in assay buffer.
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| Animal Protocol |
In vivo animal studies for Gly-AMC are not typically performed, as the compound is primarily used as a research tool in biochemical assays rather than as a therapeutic agent. The compound is not intended for in vivo use or for therapeutic applications. Gly-AMC is a valuable tool for studying enzyme function and regulation in vitro. Further studies may explore the use of Gly-AMC in more complex biological systems, but these are not extensively documented.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Gly-AMC are not applicable, as the compound is primarily used as a research tool in biochemical assays rather than as a therapeutic agent. The compound is not intended for in vivo use, and its pharmacokinetic properties have not been characterized. Gly-AMC is a small molecule with a molecular weight of 268.7 g/mol and a molecular formula of C12H13ClN2O3. Its solubility and stability are important factors for its use in biochemical assays. Research-grade Gly-AMC is intended for laboratory use only.
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| Toxicity/Toxicokinetics |
Toxicological data for Gly-AMC are not applicable, as the compound is primarily used as a research tool in biochemical assays rather than as a therapeutic agent. The compound is not intended for in vivo use, and its toxicological properties have not been characterized. As a research chemical, Gly-AMC is intended for laboratory use only and is not for human consumption. As with all research chemicals, appropriate safety precautions should be taken when handling Gly-AMC.
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| Additional Infomation |
Gly-AMC (H-Gly-AMC) is a probe and chromophore substrate for aminopeptidase. It has the molecular formula C12H13ClN2O3 and CAS number 208459-17-0. Gly-AMC is used to make Ub-AMC, a probe for deubiquitinating enzyme (DUB) activity. It is used in enzyme specificity studies and inhibitor screening. Gly-AMC is not a drug and is strictly a research compound for biochemical assays.
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| Molecular Formula |
C12H13CLN2O3
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| Molecular Weight |
268.697
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| Exact Mass |
268.061
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| Elemental Analysis |
C, 53.64; H, 4.88; Cl, 13.19; N, 10.43; O, 17.86
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| CAS # |
208459-17-0
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| Related CAS # |
77471-42-2;208459-17-0 (HCl);
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| PubChem CID |
134128878
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| Appearance |
Solid powder
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
18
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| Complexity |
365
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| Defined Atom Stereocenter Count |
0
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| SMILES |
Cl.O1C(C=C(C)C2C=CC(=CC1=2)NC(CN)=O)=O
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| InChi Key |
DGTMMNCLSKYGIF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H12N2O3.ClH/c1-7-4-12(16)17-10-5-8(2-3-9(7)10)14-11(15)6-13/h2-5H,6,13H2,1H3,(H,14,15)1H
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| Chemical Name |
Acetamide,2-amino-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-, monohydrochloride
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| Synonyms |
Gly-AMC HCl H-Gly-AMC HCl L-Gly-7-Amino-4-Methylcoumarin H-Gly-AMC ZINC57339 AK144290 DS-7861 DA-02879 FT-0698956
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7216 mL | 18.6081 mL | 37.2162 mL | |
| 5 mM | 0.7443 mL | 3.7216 mL | 7.4432 mL | |
| 10 mM | 0.3722 mL | 1.8608 mL | 3.7216 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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