| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
In evaluated cancer cell lines, GEM144 (0-10 μM; 24 hours) demonstrates anti-proliferative action with an IC50 of 0.26 ~ 2.2 μM[1]. In a dose-dependent manner, GEM144 (0.1 - 0.4 μM) significantly upregulates p21 expression and raises H2AX phosphorylation levels on Ser 39 (γH2AX) [1]. GEM144 causes G1/S cell cycle arrest at 0.26 μM in NCI-H4609, 0.95 μM in A2780, and 1.4 μM in MM473 over a 72-hour period [1].
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|---|---|
| ln Vivo |
In xenograft mice with a TGI of 72%, GEM144 (50 mg/kg; po, bid, 5 days per week for 3–4 weeks) dramatically decreased the MM487 tumor burden [1].
|
| Cell Assay |
Cell proliferation assay
Cell Types: NCI-H460, H460-R9A, A2780 and A2780-DX[1] Tested Concentrations: 0-10 μM Incubation Duration: 24 hrs (hours) (further growth in drug-free medium for 48 hrs (hours)) Experimental Results: Tested The IC50 of cancer cell lines is 0.26 ~ 2.2 μM. Western Blot Analysis Cell Types: NCI-H460[1] Tested Concentrations: 0.1 μM, 0.25 μM, and 0.4 μM Incubation Duration: 24 hrs (hours) Experimental Results: H2AX Ser 39 (γH2AX) phosphorylation levels were Dramatically increased and Dramatically increased at a certain dose Upregulates p21 expression in a dependent manner. Cell cycle analysis Cell Types: NCI-H4609, A2780 and MM473[1] Tested Concentrations: 0.26 μM in NCI-H4609, 0.95 μM in A2780, 1.4 μM in MM473 Incubation Duration: 72 hrs (hours) Experimental Results: Induction of G1/S cell cycle arrest. |
| Animal Protocol |
Animal/Disease Models: Female CD-1 nude mice (injected with MM473 and MM487) [1]
Doses: 50 mg/kg Route of Administration: PO, bid, 5 days a week for 3-4 weeks Experimental Results: Dramatically diminished MM487 tumors The load TGI is 72%. |
| References |
| Molecular Formula |
C28H31NO5
|
|---|---|
| Molecular Weight |
461.549448251724
|
| Exact Mass |
461.22
|
| CAS # |
2487526-28-1
|
| PubChem CID |
153307898
|
| Appearance |
White to off-white solid powder
|
| LogP |
6.1
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
34
|
| Complexity |
744
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(C(=O)NO)OC1=C(C=C(C=C1)C2=CC=C(C=C2)/C=C/C(=O)O)C34CC5CC(C3)CC(C5)C4
|
| InChi Key |
PIJPEZHKJKJZHS-RUDMXATFSA-N
|
| InChi Code |
InChI=1S/C28H31NO5/c1-17(27(32)29-33)34-25-8-7-23(22-5-2-18(3-6-22)4-9-26(30)31)13-24(25)28-14-19-10-20(15-28)12-21(11-19)16-28/h2-9,13,17,19-21,33H,10-12,14-16H2,1H3,(H,29,32)(H,30,31)/b9-4+
|
| Chemical Name |
(E)-3-[4-[3-(1-adamantyl)-4-[1-(hydroxyamino)-1-oxopropan-2-yl]oxyphenyl]phenyl]prop-2-enoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1666 mL | 10.8331 mL | 21.6661 mL | |
| 5 mM | 0.4333 mL | 2.1666 mL | 4.3332 mL | |
| 10 mM | 0.2167 mL | 1.0833 mL | 2.1666 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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