| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
α-Glucosidase; also targets cancer cell proliferation and oxidative stress pathways.
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| ln Vitro |
Ganhuangenin (Viscidulin III) inhibits the proliferation of HL-60 human leukemia cells with an IC50 of 17.4 µM. It acts as an α-glucosidase inhibitor, suggesting potential anti-diabetic activity. The compound demonstrates antibacterial activity against various pathogens, including P. acnes. Ganhuangenin enhances antioxidant enzyme activity, protecting cells from oxidative stress. These activities demonstrate the compound's potential as a multi-target natural product with anticancer, antidiabetic, antibacterial, and antioxidant properties.
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| ln Vivo |
In vivo efficacy of ganhuangenin has not been extensively characterized in publicly available literature. As an α-glucosidase inhibitor, it would be expected to show anti-hyperglycemic effects in animal models of diabetes. Its antibacterial activity suggests potential efficacy in models of bacterial infection, particularly acne. Its antioxidant properties suggest potential protective effects in models of oxidative stress-related diseases. Specific animal model data and dosing regimens are limited in publicly available sources.
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| Enzyme Assay |
α-Glucosidase inhibition is assessed using in vitro enzyme assays with α-glucosidase enzyme and a substrate such as p-nitrophenyl-α-D-glucopyranoside. The release of p-nitrophenol is measured spectrophotometrically. IC50 values are calculated from dose-response curves. Antibacterial activity is assessed using standard broth microdilution or agar diffusion methods to determine minimum inhibitory concentrations (MICs). Antioxidant activity is evaluated using cell-free assays such as DPPH or ABTS radical scavenging assays.
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| Cell Assay |
Cellular activity is evaluated in HL-60 human leukemia cells. Cells are treated with ganhuangenin at various concentrations (typically 0.1-100 µM) for 24-72 hours. Cell proliferation is measured by MTT, MTS, or CellTiter-Glo assays. Apoptosis is assessed by caspase-3/7 activation or Annexin V staining. Antioxidant effects are evaluated in cells subjected to oxidative stress by measuring reactive oxygen species (ROS) levels using fluorescent probes. Antibacterial activity is assessed in bacterial culture models.
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| Animal Protocol |
In vivo studies for ganhuangenin are limited. Potential studies could be conducted in mouse models of diabetes (e.g., streptozotocin-induced diabetes, diet-induced obesity) to assess anti-hyperglycemic effects, or in models of bacterial infection (e.g., P. acnes-induced skin infection) to assess antibacterial efficacy. The compound would be administered orally or intraperitoneally. Blood glucose, insulin levels, bacterial load, and inflammatory markers would be measured.
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| ADME/Pharmacokinetics |
Ganhuangenin (Viscidulin III) has a molecular formula of C17H14O8 and a molecular weight of 346.29 g/mol. CAS Number: 92519-91-0. Appearance: Light yellow to yellow solid powder. LogP: 1.57. Purity is typically ≥98%. The compound should be stored as a powder at -20°C for up to 3 years or at 4°C for 2 years. In solvent, it can be stored at -80°C for 6 months or at -20°C for 1 month. The product requires protection from light. It is soluble in DMSO.
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| Toxicity/Toxicokinetics |
Specific toxicological data for ganhuangenin are not extensively published. As a natural product with multiple biological activities, it should be handled with appropriate safety precautions. The compound is for research use only and not for human therapeutic applications without appropriate regulatory approval. Standard laboratory safety practices should be followed, including the use of personal protective equipment and adequate ventilation.
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| References | |
| Additional Infomation |
Reports indicate that Scutellaria baicalensis, Scutellaria baicalensis, and other organisms with available data contain glucosamine.
Ganhuangenin (Viscidulin III) is a research tool compound for studying the pharmacology of natural flavonoids. It is not approved for clinical use. The compound is a flavonoid extracted from Scutellaria baicalensis, a plant used in traditional Chinese medicine. Ganhuangenin has been investigated for its anticancer, antidiabetic (α-glucosidase inhibition), antibacterial, and antioxidant activities. Its multi-target profile makes it a valuable tool for understanding the complex pharmacology of natural products and for identifying potential lead compounds for drug development. |
| Molecular Formula |
C17H14O8
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|---|---|
| Molecular Weight |
346.29
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| Exact Mass |
346.068
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| CAS # |
92519-91-0
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| PubChem CID |
5271991
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
673.7±55.0 °C at 760 mmHg
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| Flash Point |
251.5±25.0 °C
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| Vapour Pressure |
0.0±2.1 mmHg at 25°C
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| Index of Refraction |
1.697
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| Source |
Originated from plants
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| LogP |
1.57
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
25
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| Complexity |
535
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C=C(C2=C(O)C=CC(O)=C2OC)OC3=C(OC)C(O)=CC(O)=C13
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| InChi Key |
CPFJPTACQDZPLS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H14O8/c1-23-15-8(19)4-3-7(18)14(15)12-6-10(21)13-9(20)5-11(22)16(24-2)17(13)25-12/h3-6,18-20,22H,1-2H3
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| Chemical Name |
2-(3,6-dihydroxy-2-methoxyphenyl)-5,7-dihydroxy-8-methoxychromen-4-one
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| Synonyms |
Sb-1 Sb 1 Ganhuangenin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~288.8 mM; with ultrasonication)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.22 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (7.22 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8878 mL | 14.4388 mL | 28.8775 mL | |
| 5 mM | 0.5776 mL | 2.8878 mL | 5.7755 mL | |
| 10 mM | 0.2888 mL | 1.4439 mL | 2.8878 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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