| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
Fructo-oligosaccharide DP7/GF6 targets the gut microbiome as a prebiotic. As a non-digestible carbohydrate, it passes through the upper gastrointestinal tract and is fermented by gut microbiota. This fermentation produces short-chain fatty acids that benefit gut health. The compound promotes the growth of beneficial gut bacteria. Fructooligosaccharides have been extensively studied for their prebiotic properties.
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| ln Vitro |
In vitro, Fructo-oligosaccharide DP7/GF6 is a fructooligosaccharide used in prebiotic research. It is a non-digestible carbohydrate consisting of six fructose units linked to one terminal glucose. The compound is part of the fructan family. Its prebiotic potential is assessed in fermentation studies using gut microbiota cultures. FOS have been extensively studied for their prebiotic properties.
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| ln Vivo |
In vivo, Fructo-oligosaccharide DP7/GF6 is a non-digestible carbohydrate that functions as a prebiotic. It promotes the growth of beneficial gut bacteria. As a fructooligosaccharide, it is fermented by gut microbiota to produce short-chain fatty acids that benefit gut health. The compound is widely used in prebiotic research. Further in vivo studies are ongoing to fully characterize its prebiotic effects.
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| Enzyme Assay |
In vitro enzyme assays for Fructo-oligosaccharide DP7/GF6 involve measuring its fermentation by gut microbiota. Fecal samples are cultured in anaerobic conditions with the compound as a substrate. Short-chain fatty acid production is measured by GC. For prebiotic studies, the compound's effect on gut microbiota composition is assessed by 16S rRNA sequencing. Enzyme activities involved in fructooligosaccharide metabolism may be measured. Assays are performed in appropriate buffer systems with positive controls such as known prebiotics.
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| Cell Assay |
In vitro cell-based assays for Fructo-oligosaccharide DP7/GF6 are conducted in gut epithelial cell lines and immune cells. Cells are cultured in appropriate media at 37°C with 5% CO2 and treated with the compound or its fermentation products at varying concentrations. Cell viability is assessed by standard assays. Gut barrier function is assessed by measuring transepithelial electrical resistance. Immune modulation is assessed by measuring cytokine production. Experiments are performed in triplicate with appropriate positive and negative controls.
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| Animal Protocol |
Fructo-oligosaccharide DP7/GF6 in vivo studies are conducted in animal models of gut health and metabolic diseases. Animals are fed diets containing the compound. Gut microbiota composition is assessed by 16S rRNA sequencing. Short-chain fatty acid levels in feces and blood are measured. Gut barrier function and immune parameters are assessed. For metabolic studies, body weight, glucose tolerance, and lipid profiles are measured. Animals are monitored for clinical signs. Tissues and blood samples are collected for histopathological and biochemical analysis at study endpoints.
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| ADME/Pharmacokinetics |
Fructo-oligosaccharide DP7/GF6 (MW 1153.00 g/mol, C42H72O36) is a fructooligosaccharide with DP=7. It is soluble in DMSO at 100 mg/mL. It is stable under recommended storage conditions. Fructo-oligosaccharide DP7/GF6 is a prebiotic used in gut health research. Pharmacokinetic parameters such as absorption, distribution, metabolism, and excretion would be determined in species-specific studies.
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| Toxicity/Toxicokinetics |
Fructo-oligosaccharide DP7/GF6 is generally well-tolerated as a dietary oligosaccharide. The compound is a naturally occurring fructooligosaccharide. Its prebiotic effects have been demonstrated with acceptable safety profiles. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| References | |
| Additional Infomation |
Fructo-oligosaccharide DP7/GF6 is a fructooligosaccharide with DP=7 consisting of 6 fructose units and 1 glucose unit. It is a non-digestible carbohydrate and part of the fructan family that serves as a prebiotic. The compound promotes beneficial gut bacteria growth and is used in prebiotic research. Its molecular formula is C42H72O36 with a molecular weight of 1153.00 g/mol. All applications are limited to non-human research use.
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| Molecular Formula |
C42H72O36
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|---|---|
| Molecular Weight |
1152.9995
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| Exact Mass |
1152.38
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| CAS # |
62512-20-3
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| PubChem CID |
50938613
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| Appearance |
White to off-white solid powder
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| Density |
1.89 g/cm3
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| Boiling Point |
1475.2±65.0℃ at 760 mmHg
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| Source |
Originated from plants: Gramineae Saccharum officinarum
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| LogP |
-12.9
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| Hydrogen Bond Donor Count |
23
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| Hydrogen Bond Acceptor Count |
36
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| Rotatable Bond Count |
25
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| Heavy Atom Count |
78
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| Complexity |
1920
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| Defined Atom Stereocenter Count |
29
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| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@]2([C@H]([C@@H]([C@H](O2)CO)O)O)CO[C@]3([C@H]([C@@H]([C@H](O3)CO)O)O)CO[C@]4([C@H]([C@@H]([C@H](O4)CO)O)O)CO[C@]5([C@H]([C@@H]([C@H](O5)CO)O)O)CO[C@]6([C@H]([C@@H]([C@H](O6)CO)O)O)CO[C@]7([C@H]([C@@H]([C@H](O7)CO)O)O)CO)O)O)O)O
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| InChi Key |
RUCFLBDDUFAPAQ-LYUODHIZSA-N
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| InChi Code |
InChI=1S/C42H72O36/c43-1-14-21(51)28(58)29(59)36(71-14)78-42(35(65)27(57)20(7-49)77-42)13-70-41(34(64)26(56)19(6-48)76-41)12-69-40(33(63)25(55)18(5-47)75-40)11-68-39(32(62)24(54)17(4-46)74-39)10-67-38(31(61)23(53)16(3-45)73-38)9-66-37(8-50)30(60)22(52)15(2-44)72-37/h14-36,43-65H,1-13H2/t14-,15-,16-,17-,18-,19-,20-,21-,22-,23-,24-,25-,26-,27-,28+,29-,30+,31+,32+,33+,34+,35+,36-,37-,38-,39-,40-,41-,42+/m1/s1
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| Chemical Name |
(2R,3R,4S,5S,6R)-2-[(2S,3S,4S,5R)-2-[[(2R,3S,4S,5R)-2-[[(2R,3S,4S,5R)-2-[[(2R,3S,4S,5R)-2-[[(2R,3S,4S,5R)-2-[[(2R,3S,4S,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~86.7 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.17 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.17 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (2.17 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8673 mL | 4.3365 mL | 8.6730 mL | |
| 5 mM | 0.1735 mL | 0.8673 mL | 1.7346 mL | |
| 10 mM | 0.0867 mL | 0.4337 mL | 0.8673 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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