| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Flutriafol targets fungal sterol 14α-demethylase (CYP51), an enzyme essential for ergosterol biosynthesis. By inhibiting CYP51, flutriafol blocks the demethylation of lanosterol, leading to the depletion of ergosterol and accumulation of toxic sterol intermediates. This disruption compromises fungal cell membrane integrity, resulting in fungal growth inhibition and cell death. As a DMI fungicide, flutriafol is effective against a broad range of plant pathogenic fungi.
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| ln Vitro |
In vitro, flutriafol demonstrates broad-spectrum fungicidal activity against various plant pathogenic fungi. Its activity is assessed by measuring inhibition of mycelial growth in agar-based assays or by determining minimum inhibitory concentrations (MIC) against fungal isolates. The compound is effective against rusts, powdery mildew, and leaf spot pathogens. Its fungicidal activity is concentration-dependent, with effective control achieved at low concentrations.
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| ln Vivo |
In vivo, flutriafol is applied as a systemic fungicide for the control of fungal diseases in cereal crops, grapes, and soybeans. It is absorbed by plant tissues and translocated throughout the plant, providing protective and curative activity. The compound is effective in controlling cereal powdery mildew, cloud disease, leaf spot disease, and rust disease. Its systemic properties allow for effective disease control even when applied before or after infection.
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| Enzyme Assay |
In vitro enzyme assays for flutriafol measure its inhibition of fungal CYP51 activity. The enzyme is obtained from fungal microsomal preparations and incubated with its substrate in the presence of NADPH and varying concentrations of the compound. The production of ergosterol or the accumulation of 14α-methylated sterols is quantified by HPLC or GC-MS. IC50 values are calculated from dose-response curves. The compound's binding to CYP51 can be assessed using spectroscopic methods.
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| Cell Assay |
In vitro cell-based assays for flutriafol use fungal cultures of plant pathogenic species. Fungal spores or mycelia are inoculated into liquid or solid media containing serial dilutions of the compound. Antifungal activity is assessed by measuring inhibition of mycelial growth or by determining minimum inhibitory concentrations using broth microdilution methods. Fungicidal activity is evaluated by subculturing onto drug-free media. The compound's effects on ergosterol content can also be quantified.
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| Animal Protocol |
In vivo plant assays for flutriafol involve the treatment of infected crops in greenhouse or field settings. The compound is applied as a foliar spray or seed treatment at various concentrations. Efficacy is evaluated by assessing disease severity, measuring lesion size, or quantifying fungal biomass on treated versus untreated plants. Dose-response studies establish the effective concentration for disease control. The compound's systemic translocation and residual activity are also assessed.
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| ADME/Pharmacokinetics |
Flutriafol is absorbed by plant roots and foliage and translocated systemically throughout the plant. Its persistence in plants and soil depends on environmental factors such as temperature, moisture, and soil type. The compound has a moderate half-life in soil and is subject to degradation by microbial and chemical processes. Residue levels on treated crops are monitored to ensure compliance with maximum residue limits for food safety.
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| Toxicity/Toxicokinetics |
Flutriafol has low acute toxicity to mammals but is classified as hazardous to aquatic organisms. Occupational exposure may cause skin and eye irritation. Chronic toxicity studies in animals have been conducted to establish acceptable daily intake levels for human consumption of treated produce. The compound is not considered genotoxic or carcinogenic at relevant exposure levels. Appropriate safety precautions should be taken during handling and application.
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| Additional Infomation |
1-(2-fluorophenyl)-1-(4-fluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethanol is a tertiary alcohol with the structure of an ethanol molecule, wherein one hydrogen atom at position 1 is replaced by a p-fluorophenyl group, the other hydrogen atom at position 1 is also replaced by a p-fluorophenyl group, and one hydrogen atom at position 2 is replaced by a 1H-1,2,4-triazol-1-yl group. It belongs to the triazole class of compounds, tertiary alcohols, and monofluorobenzenes.
Flutriafol is registered and marketed as a systemic fungicide for agricultural use in many countries. It is used on a variety of crops including cereals, grapes, soybeans, and other commodities. The compound belongs to the triazole class of fungicides and acts as a sterol demethylation inhibitor. Its broad-spectrum activity and systemic properties make it a valuable tool for integrated pest management in agriculture. |
| Molecular Formula |
C16H13F2N3O
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|---|---|
| Molecular Weight |
301.29
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| Exact Mass |
301.102
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| CAS # |
76674-21-0
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| PubChem CID |
91727
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
506.5±60.0 °C at 760 mmHg
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| Melting Point |
130ºC
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| Flash Point |
260.1±32.9 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.600
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| LogP |
2.31
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
22
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| Complexity |
365
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C(C(=C1)C(CN2C=NC=N2)(C3=CC=C(C=C3)F)O)F
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| InChi Key |
JWUCHKBSVLQQCO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H13F2N3O/c17-13-7-5-12(6-8-13)16(22,9-21-11-19-10-20-21)14-3-1-2-4-15(14)18/h1-8,10-11,22H,9H2
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| Chemical Name |
1-(2-fluorophenyl)-1-(4-fluorophenyl)-2-(1,2,4-triazol-1-yl)ethanol
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| Synonyms |
PP 450; Impact; Flutriafol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~829.77 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3191 mL | 16.5953 mL | 33.1906 mL | |
| 5 mM | 0.6638 mL | 3.3191 mL | 6.6381 mL | |
| 10 mM | 0.3319 mL | 1.6595 mL | 3.3191 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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