| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
Fluorescent Red Mega 480 does not have a specific biological target. Its primary function is as a fluorescent label for biological imaging and detection applications. The large Stokes shift allows for efficient separation of excitation and emission light, reducing background and enabling multiplexing. It is designed for use in fluorescence microscopy, flow cytometry, and other fluorescence-based techniques.
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| ln Vitro |
In vitro, Fluorescent Red Mega 480 is used as a fluorescent label for staining cells, tissues, or biomolecules. Its large Stokes shift makes it particularly useful for multicolor experiments where multiple fluorophores are used simultaneously. Specific in vitro activity data, such as effects on cells, are not applicable as it is a fluorescent dye rather than a pharmacologically active compound.
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| ln Vivo |
In vivo applications of Fluorescent Red Mega 480 are not specifically described in the available sources. As a fluorescent dye, it could potentially be used for in vivo imaging, such as tracking cells or monitoring biological processes in animal models. However, its primary use is in in vitro and ex vivo applications. Specific in vivo data are not provided.
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| Enzyme Assay |
A cell-free assay for Fluorescent Red Mega 480 is not applicable as it is not an enzyme inhibitor or receptor ligand. Its properties are characterized by spectroscopic methods. Its excitation and emission spectra can be measured in solution to confirm the large Stokes shift. The dye's fluorescence quantum yield and photostability can also be assessed in cell-free systems.
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| Cell Assay |
Cellular experiments with Fluorescent Red Mega 480 typically involve staining cells for fluorescence microscopy or flow cytometry. Cells are incubated with the dye at appropriate concentrations, washed, and then analyzed. The large Stokes shift allows for the use of standard fluorescein filter sets for excitation. Specific protocols depend on the application and cell type.
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| Animal Protocol |
In vivo animal experiments for Fluorescent Red Mega 480 are not described in the available sources. As a fluorescent dye, it could potentially be used for in vivo imaging studies, but specific protocols and data are not provided.
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| ADME/Pharmacokinetics |
Fluorescent Red Mega 480 is a small molecule dye with a molecular weight of 514.6. Its pharmacokinetic properties are not reported in the available sources. As a fluorescent dye used in research, its biodistribution and clearance are not typically characterized unless it is used for in vivo imaging applications. The dye is soluble in appropriate solvents for biological applications.
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| Toxicity/Toxicokinetics |
Toxicity data for Fluorescent Red Mega 480 are not provided in the available sources. As a fluorescent dye, it is generally considered to have low toxicity for its intended research applications. However, specific toxicological information is not available. The compound is intended for research use only and not for human consumption. Standard laboratory safety precautions should be observed when handling this compound.
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| References | |
| Additional Infomation |
DY-480XL is an imine betaine that can be used as a fluorescent dye.
Fluorescent Red Mega 480 (CAS: 540528-00-5) is a red fluorescent dye with an exceptionally large Stokes shift, designed for multicolor fluorescence applications. It belongs to the Mega dye family and is optimized for high-plex, multicolor experiments by minimizing spectral crosstalk. The dye can be excited using the same light source or filter settings as fluorescein. Its molecular formula is C₂₆H₃₀N₂O₇S with a molecular weight of 514.6. It is not a therapeutic drug and is strictly for research purposes. |
| Molecular Formula |
C26H30N2O7S
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|---|---|
| Exact Mass |
514.177
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| CAS # |
540528-00-5
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| PubChem CID |
16212651
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
2.587
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
36
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| Complexity |
910
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)O2)C=CC3=[N+](C=C(C=C3)S(=O)(=O)[O-])CCCCCC(=O)O
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| InChi Key |
XWKHECGJHWMWTB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C26H30N2O7S/c1-3-27(4-2)22-12-9-19-16-20(26(31)35-24(19)17-22)10-11-21-13-14-23(36(32,33)34)18-28(21)15-7-5-6-8-25(29)30/h9-14,16-18H,3-8,15H2,1-2H3,(H-,29,30,32,33,34)
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| Chemical Name |
1-(5-carboxypentyl)-6-[(E)-2-[7-(diethylamino)-2-oxochromen-3-yl]ethenyl]pyridin-1-ium-3-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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