| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
Fluorescein Sodium does not have a specific biological target; it is a fluorescent tracer used for visualization purposes. It is a pH-sensitive fluorophore whose fluorescence is maximal at basic pH. Its fluorescence is based on its chemical structure rather than binding to a specific receptor or enzyme.
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|---|---|
| ln Vitro |
Fluorescein is an artificially created organic photoactive dye chemical that dissolves in solvents such as methanol, alcohol, and water [1].
Fluorescein Sodium is a photoactive dye chemical that dissolves in solvents such as methanol, alcohol, and water. It exhibits intense green fluorescence under blue light excitation. The compound has been extensively used as a scaffold for the development of practically useful green fluorescent probes. |
| ln Vivo |
Fluorescein Sodium is used in vivo as a fluorescent tracer in medical and biological applications, including angiography, tumor-infected tissue tracing, and as a diagnostic aid. It is also used in ophthalmology for fluorescein angiography to visualize retinal blood vessels. The compound is administered intravenously or topically for these applications.
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| Enzyme Assay |
For non-cellular assays, Fluorescein Sodium can be used as a fluorescent tracer in cell-free systems. Its fluorescence intensity is measured spectrophotometrically, and the compound serves as a standard for fluorescence calibration. It can be used to study membrane permeability in artificial membrane systems or to assess drug release from formulations in dissolution testing.
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| Cell Assay |
In vitro cellular assays with Fluorescein Sodium involve treating cells with the compound to assess cellular uptake, membrane integrity, or as a model drug for permeability studies. The compound is also used to quantify root dentin demineralization in dental research. Fluorescence is measured using a plate reader or fluorescence microscopy with excitation/emission appropriate for fluorescein (∼495/520 nm).
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| Animal Protocol |
In vivo animal experiments with Fluorescein Sodium involve intravenous or topical administration to animals for imaging purposes. The compound is used as a tracer to study vascular permeability, tissue perfusion, and tumor localization. In ophthalmology research, it is used for fundus angiography in animal models. The dye’s distribution is monitored using fluorescence imaging techniques.
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| ADME/Pharmacokinetics |
Fluorescein Sodium has a molecular weight of 376.27 and is highly water-soluble with a solubility of 500 g/L at 20°C. It is rapidly distributed in the extracellular space after intravenous administration and is excreted primarily by the kidneys, giving urine a characteristic yellow-green color. The compound has a short half-life in circulation.
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| Toxicity/Toxicokinetics |
Fluorescein Sodium is considered to have low toxicity at diagnostic doses. Adverse reactions are rare but can include nausea, vomiting, and allergic reactions including anaphylaxis in sensitive individuals. The compound is generally recognized as safe for use as a diagnostic agent. It is not intended for therapeutic use and is for research and diagnostic purposes only.
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| References |
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| Additional Infomation |
See also: Sodium fluorescein (note moved to).
Fluorescein Sodium is widely used as a fluorescent tracer in medical diagnostics, including fluorescein angiography for retinal examination. It is also used in industrial applications such as dyeing silk and wool, ink manufacturing, cosmetics coloring, and as a toner. The compound is a standard green fluorophore in fluorescence microscopy and flow cytometry. It is available for research and diagnostic use. |
| Molecular Formula |
C20H10O5NA2
|
|---|---|
| Molecular Weight |
376.27
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| Exact Mass |
376.032
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| CAS # |
518-47-8
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| Related CAS # |
Fluorescein;2321-07-5
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| PubChem CID |
9885981
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| Appearance |
Brown to reddish brown solid powder
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| Density |
1.601 g/cm3 (20ºC)
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| Boiling Point |
620.8ºC at 760 mmHg
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| Melting Point |
320 °C
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| Flash Point |
232.6ºC
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| LogP |
4.542
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
27
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| Complexity |
522
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
RGPLGPBQJOQFJS-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
|
| Chemical Name |
disodium;3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~20.83 mg/mL (~55.36 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6577 mL | 13.2883 mL | 26.5767 mL | |
| 5 mM | 0.5315 mL | 2.6577 mL | 5.3153 mL | |
| 10 mM | 0.2658 mL | 1.3288 mL | 2.6577 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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