| Size | Price | |
|---|---|---|
| 100mg | ||
| Other Sizes |
| Targets |
There is no pharmacological receptor target; the acetate group is hydrolyzed by intracellular esterases to release fluorescein, which is retained in cells with intact membranes and reports cell viability, membrane integrity, and esterase activity. The fluorescein moiety can also be activated to react with amines for isomerically homogeneous fluorescent labeling of biomolecules.
|
|---|---|
| ln Vitro |
In vitro, fluorescein-O-acetate is taken up by cells and hydrolyzed to fluorescein, producing bright fluorescence that allows assessment of cellular uptake and viability; it is used in cell proliferation and cytotoxicity assays, and as a fluorescent label for detecting and quantifying chemical reactions, with applications in fluorescence microscopy, flow cytometry, and ophthalmologic diagnostics.
|
| ln Vivo |
In vivo, fluorescein-O-acetate is used as a diagnostic tool in ophthalmology for fluorescein angiography to visualize blood vessels and tissues; it is also used for cell tracking in animal models, where labeled cells are detected by fluorescence imaging, and its hydrolysis product fluorescein is cleared renally, enabling visualization of vascular and renal function.
|
| Enzyme Assay |
Fluorescein-O-acetate is characterized by spectrophotometry (excitation/emission maxima of fluorescein, ~490/520 nm) and HPLC for purity; esterase activity is measured by incubating the substrate with cell lysates or purified esterases and monitoring the increase in fluorescence at 520 nm, and the rate of hydrolysis is quantified against a fluorescein standard curve.
|
| Cell Assay |
Cells are incubated with fluorescein-O-acetate (typically 0.1–10 µM) for 15–60 min, washed, and analyzed by fluorescence microscopy or flow cytometry; intracellular fluorescein fluorescence reports esterase activity and membrane integrity, and cell viability is quantified by comparing fluorescence intensity with untreated controls.
|
| Animal Protocol |
Mice or rats receive fluorescein-O-acetate by intravenous or local administration for fluorescein angiography or cell tracking; vascular and tissue fluorescence is imaged by fundus angiography or whole-body fluorescence imaging, and plasma clearance and renal excretion of fluorescein are measured by fluorescence spectroscopy of blood and urine samples.
|
| References |
| Molecular Formula |
C22H14O7
|
|---|---|
| Molecular Weight |
390.34236
|
| Exact Mass |
390.074
|
| CAS # |
233759-98-3
|
| PubChem CID |
16218651
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.55g/cm3
|
| Boiling Point |
718.2ºC at 760 mmHg
|
| Flash Point |
259.6ºC
|
| Vapour Pressure |
1.19E-21mmHg at 25°C
|
| Index of Refraction |
1.721
|
| LogP |
3.726
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
29
|
| Complexity |
806
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(O)(=O)COC1=CC2OC3=C(C4(C=2C=C1)C1=C(C=CC=C1)C(=O)O4)C=CC(O)=C3
|
| InChi Key |
VQKDLLIKXLDPHW-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C22H14O7/c23-12-5-7-16-18(9-12)29-19-10-13(28-11-20(24)25)6-8-17(19)21(16)14-3-1-2-4-15(14)22(26)27/h1-10H,11H2,(H,24,25)(H,26,27)
|
| Chemical Name |
2-[3-(carboxymethoxy)-6-oxoxanthen-9-yl]benzoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5619 mL | 12.8093 mL | 25.6187 mL | |
| 5 mM | 0.5124 mL | 2.5619 mL | 5.1237 mL | |
| 10 mM | 0.2562 mL | 1.2809 mL | 2.5619 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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