| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Fluorescein Biotin targets the biotin-binding sites of avidin and streptavidin. The compound is a ligand for avidin and streptavidin, which are proteins that bind biotin with extremely high affinity. The biotin moiety of Fluorescein Biotin binds to the biotin-binding pocket of avidin or streptavidin. This binding induces significant fluorescence quenching. The compound is used as a fluorescent probe for detecting and quantifying biotin-binding sites.
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|---|---|
| ln Vitro |
In vitro, Fluorescein Biotin is used as a fluorescent probe for detecting and quantifying biotin-binding sites by fluorescence or absorbance. Upon binding to avidin or streptavidin, the fluorescence or absorbance of Fluorescein Biotin is quenched. This property makes it useful for studying biotin-binding proteins and for developing detection assays. The compound is also used in fluorescence microscopy and flow cytometry. No other in vitro activities have been reported.
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| ln Vivo |
In vivo activity of Fluorescein Biotin has not been characterized. The compound is used as a detection reagent in vitro and is not intended for in vivo administration. No animal studies have been conducted to evaluate its pharmacokinetics, efficacy, or safety in vivo. The compound is used exclusively in laboratory detection applications.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for Fluorescein Biotin typically involve binding studies with avidin or streptavidin. The compound is incubated with avidin or streptavidin in appropriate buffer. Binding is detected by measuring the fluorescence intensity at 485/535 nm or absorbance. The fluorescence quenching upon binding is monitored. The binding affinity can be determined by titration experiments. Competition assays with unlabeled biotin can be used to assess specificity.
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| Cell Assay |
In vitro cell-based assays for Fluorescein Biotin typically involve staining cells or cell lysates. The compound is added to cells or samples, and binding to biotin-binding proteins is detected by fluorescence microscopy or flow cytometry. The fluorescence quenching upon binding can be used to detect the presence of avidin or streptavidin. The compound is also used in ELISA and other immunoassay formats.
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| Animal Protocol |
In vivo animal studies are not applicable to Fluorescein Biotin, as the compound is used as a detection reagent in vitro. No animal studies have been conducted. The compound is not administered to animals for research purposes. It is used exclusively in laboratory applications.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) properties of Fluorescein Biotin have not been characterized, as the compound is not intended for in vivo use. No absorption, distribution, metabolism, or excretion studies have been conducted. The compound is used as a detection reagent in vitro and is not administered to animals. Its PK properties are not relevant to its intended applications.
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| Toxicity/Toxicokinetics |
The toxicity profile of Fluorescein Biotin has not been extensively characterized. The compound is used as a detection reagent in vitro and is not intended for in vivo administration. Standard safety precautions for handling fluorescent dyes should be followed. The compound is intended for research use only.
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| References | |
| Additional Infomation |
Fluorescein Biotin is a research-grade fluorescent biotin derivative used as a detection reagent. It binds to avidin and streptavidin with fluorescence quenching. The compound displays excitation/emission maxima of 485/535 nm. It is used in fluorescence microscopy, flow cytometry, and immunoassays. The compound is not an approved therapeutic drug. Molecular formula: C₄₂H₅₀N₆O₈S₂; molecular weight: 831.01. Synonyms include FITC biotin. It should be stored appropriately.
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| Molecular Formula |
C42H50N6O8S2
|
|---|---|
| Molecular Weight |
831.0118
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| Exact Mass |
830.313
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| CAS # |
134759-22-1
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| PubChem CID |
90469808
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| Appearance |
Yellow to orange solid powder
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| Density |
1.43 g/cm3
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| Index of Refraction |
1.699
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| LogP |
7.544
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| Hydrogen Bond Donor Count |
8
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
18
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| Heavy Atom Count |
58
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| Complexity |
1430
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCCCNC(=S)NC3=CC4=C(C=C3)C5(C6=C(C=C(C=C6)O)OC7=C5C=CC(=C7)O)OC4=O)NC(=O)N2
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| InChi Key |
MFVSXUVAYGNGJQ-INIOENKZSA-N
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| InChi Code |
InChI=1S/C42H50N6O8S2/c49-26-13-16-30-33(22-26)55-34-23-27(50)14-17-31(34)42(30)29-15-12-25(21-28(29)39(53)56-42)46-41(57)45-20-8-2-7-19-44-36(51)10-3-1-6-18-43-37(52)11-5-4-9-35-38-32(24-58-35)47-40(54)48-38/h12-17,21-23,32,35,38,49-50H,1-11,18-20,24H2,(H,43,52)(H,44,51)(H2,45,46,57)(H2,47,48,54)/t32-,35-,38-/m0/s1
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| Chemical Name |
6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-N-[5-[(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)carbamothioylamino]pentyl]hexanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2034 mL | 6.0168 mL | 12.0335 mL | |
| 5 mM | 0.2407 mL | 1.2034 mL | 2.4067 mL | |
| 10 mM | 0.1203 mL | 0.6017 mL | 1.2034 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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