| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Fungal lanosterol 14α-demethylase (CYP51).
|
|---|---|
| ln Vitro |
Fluconazole hydrate inhibits fungal lanosterol 14α-demethylase, blocking ergosterol synthesis and disrupting fungal cell membrane integrity. It is a triazole antifungal agent with excellent activity against a broad range of fungi, especially Candida albicans. The compound demonstrates potent antifungal activity in standard mycological media.
|
| ln Vivo |
Fluconazole hydrate is clinically used for the treatment and prevention of superficial and systemic fungal infections. It is orally bioavailable and well absorbed, with excellent tissue penetration. The compound is effective against a broad range of pathogenic fungi, particularly Candida species.
|
| Enzyme Assay |
The lanosterol 14α-demethylase assay uses recombinant fungal CYP51 enzyme and lanosterol as substrate. The reaction is monitored by measuring the formation of ergosterol precursors using HPLC or GC-MS. Test compounds are incubated with the enzyme and substrate, and IC50 values are determined from inhibition curves.
|
| Cell Assay |
In cellular assays, Candida albicans or other fungal strains are cultured in the presence of Fluconazole hydrate. Fungal growth is assessed by measuring optical density or CFU counts. Ergosterol content is quantified by HPLC to confirm target engagement and mechanism of action.
|
| Animal Protocol |
In vivo studies use mouse models of systemic or mucosal candidiasis. Fluconazole hydrate is administered orally, and fungal burden in kidneys, spleen, and other organs is measured by CFU counting. Survival rates and histopathological changes are assessed to evaluate antifungal efficacy.
|
| References |
Antimicrob Agents Chemother.2008 Mar;52(3):1127-32;Antimicrob Agents Chemother.1996 Sep;40(9):2178-82.
|
| Additional Infomation |
See also: Fluconazole (note moved to).
|
| Molecular Formula |
C₁₃H₁₄F₂N₆O₂
|
|---|---|
| Molecular Weight |
324.29
|
| Exact Mass |
324.114
|
| CAS # |
155347-36-7
|
| Related CAS # |
Fluconazole;86386-73-4;Fluconazole mesylate;159532-41-9
|
| PubChem CID |
9862025
|
| Appearance |
Typically exists as solid at room temperature
|
| tPSA |
82.6
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
23
|
| Complexity |
358
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
FC1=CC=C(C(O)(CN2C=NC=N2)CN3C=NC=N3)C(F)=C1.O
|
| InChi Key |
QSZFKRINVAUJGQ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C13H12F2N6O.H2O/c14-10-1-2-11(12(15)3-10)13(22,4-20-8-16-6-18-20)5-21-9-17-7-19-21;/h1-3,6-9,22H,4-5H2;1H2
|
| Chemical Name |
2-(2,4-difluorophenyl)-1,3-bis(1,2,4-triazol-1-yl)propan-2-ol;hydrate
|
| Synonyms |
UK 49858 hydrateUK49858 hydrateUK-49858 hydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0837 mL | 15.4183 mL | 30.8366 mL | |
| 5 mM | 0.6167 mL | 3.0837 mL | 6.1673 mL | |
| 10 mM | 0.3084 mL | 1.5418 mL | 3.0837 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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