| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Florfenicol-d3 targets the same bacterial ribosome as unlabeled Florfenicol. Florfenicol inhibits bacterial protein synthesis by binding to the 50S and 70S ribosomal subunits. As a deuterated analog, Florfenicol-d3 is primarily used as an analytical standard rather than for studying antibacterial activity.
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|---|---|
| ln Vitro |
In vitro, Florfenicol-d3 is used as an internal standard in analytical assays for the quantification of florfenicol in biological and environmental samples. The compound is added to samples during the extraction process, and its recovery is monitored to correct for losses during sample preparation. Its in vitro activity is purely analytical; it does not exert any pharmacological effects.
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| ln Vivo |
In vivo, Florfenicol-d3 is not administered as a therapeutic agent. It is used as an analytical standard for monitoring florfenicol residues in food products derived from animals that may have been treated with the antibiotic. The deuterated label allows for accurate quantification of florfenicol residues by LC-MS/MS, ensuring that food products meet safety standards.
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| Enzyme Assay |
The in vitro analytical assay for Florfenicol-d3 involves the extraction of florfenicol from food samples (e.g., meat, milk, fish). The deuterated internal standard is added before extraction. Samples are extracted with organic solvent and analyzed by LC-MS/MS. The compound is quantified by monitoring specific mass transitions for the deuterated and unlabeled compounds.
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| Cell Assay |
For in vitro cellular assays, Florfenicol-d3 is not typically used, as it is an analytical standard rather than a compound with biological activity. Its primary application is in analytical chemistry and food safety testing.
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| Animal Protocol |
For in vivo studies, Florfenicol-d3 is not administered to animals as a therapeutic agent. It is used in food safety testing to monitor florfenicol residues in animal-derived food products.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Florfenicol-d3 are not relevant, as it is an analytical standard rather than a therapeutic agent. The compound has a molecular weight of 361.23. When used as an internal standard, its behavior in analytical procedures is identical to that of unlabeled florfenicol.
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| Toxicity/Toxicokinetics |
Toxicology data for Florfenicol-d3 are not relevant, as it is an analytical standard used at trace levels. Florfenicol is a commonly used veterinary antibiotic. The deuterated internal standard is used to ensure accurate quantification of florfenicol residues in food safety testing.
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| References | |
| Additional Infomation |
Florfenicol-d3 is a deuterated version of Florfenicol, a synthetic antibiotic belonging to the amphenicol class. It has a molecular formula of C12H11D3Cl2FNO4S and a molecular weight of 361.23. Florfenicol-d3 is intended for use as an internal standard for the quantification of florfenicol by GC- or LC-MS. It is not approved for human use and is intended for analytical research purposes only.
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| Molecular Formula |
C12H14CL2FNO4S
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|---|---|
| Molecular Weight |
361.2317
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| Exact Mass |
360.019
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| CAS # |
2213400-85-0
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| Related CAS # |
Florfenicol;73231-34-2
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| PubChem CID |
156589023
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
617.5±55.0 °C at 760 mmHg
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| Flash Point |
327.3±31.5 °C
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| Vapour Pressure |
0.0±1.9 mmHg at 25°C
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| Index of Refraction |
1.548
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| LogP |
-0.12
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
21
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| Complexity |
447
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| Defined Atom Stereocenter Count |
2
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| SMILES |
ClC([H])(C(N([H])[C@]([H])(C([H])([H])F)[C@@]([H])(C1C([H])=C([H])C(=C([H])C=1[H])S(C([2H])([2H])[2H])(=O)=O)O[H])=O)Cl
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| InChi Key |
AYIRNRDRBQJXIF-BFHKXKNSSA-N
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| InChi Code |
InChI=1S/C12H14Cl2FNO4S/c1-21(19,20)8-4-2-7(3-5-8)10(17)9(6-15)16-12(18)11(13)14/h2-5,9-11,17H,6H2,1H3,(H,16,18)/t9-,10-/m1/s1/i1D3
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| Chemical Name |
2,2-dichloro-N-[(1R,2S)-3-fluoro-1-hydroxy-1-[4-(trideuteriomethylsulfonyl)phenyl]propan-2-yl]acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7683 mL | 13.8416 mL | 27.6832 mL | |
| 5 mM | 0.5537 mL | 2.7683 mL | 5.5366 mL | |
| 10 mM | 0.2768 mL | 1.3842 mL | 2.7683 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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