| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 100mg | |||
| 250mg | |||
| Other Sizes |
| Targets |
FlAsH-EDT2 targets tetracysteine motifs (Cys-Cys-X-X-Cys-Cys, or CCPGCC) that are engineered into recombinant proteins. The biarsenical compound binds covalently to the two pairs of cysteine residues with high affinity and specificity. Upon binding, the fluorescence of the arsenical fluorophore is dramatically enhanced, allowing the visualization of the tagged protein in living cells.
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| ln Vitro |
FlAsH-EDT2 is a pro-fluorescent compound that becomes fluorescent only upon binding to its tetracysteine target. It binds to Cys4 motifs with high affinity and emits fluorescence. The compound can also bind non-specifically to endogenous cysteine-rich proteins, but its selectivity for the tetracysteine motif is sufficient for many applications. It is useful for labeling recombinant proteins that express at very high levels.
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| ln Vivo |
FlAsH-EDT2 is not used as a therapeutic agent but as a research tool for protein labeling and imaging in living cells. It can cross the cell membrane and enter the interior of the cell, making it suitable for live-cell imaging applications. The compound is used to study protein dynamics, trafficking, and localization in real time.
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| Enzyme Assay |
For non-cellular assays, FlAsH-EDT2 is used to label purified proteins containing a tetracysteine tag. The protein is incubated with FlAsH-EDT2 in the presence of a reducing agent (e.g., DTT or TCEP) to maintain the cysteine residues in the reduced state. The labeled protein is then analyzed by SDS-PAGE with fluorescence scanning or by fluorescence spectroscopy to confirm labeling and to assess the stoichiometry of labeling.
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| Cell Assay |
In vitro cellular assays with FlAsH-EDT2 involve expressing a tetracysteine-tagged protein of interest in cultured cells. Cells are incubated with FlAsH-EDT2, which crosses the cell membrane and binds to the tetracysteine tag. After washing to remove unbound dye, the cells are imaged by fluorescence microscopy to visualize the localization and dynamics of the tagged protein. The compound's fluorescence can also be quantified by flow cytometry.
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| Animal Protocol |
FlAsH-EDT2 is not typically used in animal experiments as a therapeutic agent. It is a research reagent that may be used for ex vivo imaging of tissues from animals expressing tetracysteine-tagged proteins. In some studies, animals may be injected with FlAsH-EDT2 for in vivo imaging of tagged proteins, but this is not a standard application. The compound's membrane permeability makes it suitable for such studies.
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| ADME/Pharmacokinetics |
FlAsH-EDT2 has a molecular weight of 664.50 and a molecular formula of C₂₄H₁₈As₂O₅S₄. It is a solid at room temperature and is typically stored at -20°C, protected from light. The compound is soluble in DMSO and other organic solvents. Purity is typically ≥95%. It is moisture-sensitive and should be stored in a desiccated environment.
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| Toxicity/Toxicokinetics |
FlAsH-EDT2 contains arsenic and is toxic. It should be handled with extreme care using appropriate personal protective equipment. The compound is intended for research use only and is not for human therapeutic or diagnostic use. Standard laboratory safety precautions for handling toxic compounds should be followed.
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| References |
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| Additional Infomation |
FlAsH-EDT2 is a widely used tool for labeling tetracysteine-tagged proteins in living cells. It is a membrane-permeable, pro-fluorescent biarsenical compound that becomes fluorescent upon binding to its target. It is used to study protein dynamics and localization. The compound is available from various commercial suppliers.
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| Molecular Formula |
C24H18AS2O5S4
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|---|---|
| Molecular Weight |
664.49
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| Exact Mass |
663.847
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| CAS # |
212118-77-9
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| PubChem CID |
9831137
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| Appearance |
White to light yellow solid powder
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| Boiling Point |
849.8ºC at 760 mmHg
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| Flash Point |
467.8ºC
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| Vapour Pressure |
1.02E-30mmHg at 25°C
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| LogP |
4.016
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
35
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| Complexity |
784
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
AJNUQUGWNQHQDJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H18As2O5S4/c27-17-7-5-15-21(19(17)25-32-9-10-33-25)30-22-16(6-8-18(28)20(22)26-34-11-12-35-26)24(15)14-4-2-1-3-13(14)23(29)31-24/h1-8,27-28H,9-12H2
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| Chemical Name |
4',5'-bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
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| Synonyms |
FlAsH-EDT-2; FlAsH EDT2; FlAsH-EDT2
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5049 mL | 7.5246 mL | 15.0491 mL | |
| 5 mM | 0.3010 mL | 1.5049 mL | 3.0098 mL | |
| 10 mM | 0.1505 mL | 0.7525 mL | 1.5049 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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