| Size | Price | Stock | Qty |
|---|---|---|---|
| 50g |
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| 100g |
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| Other Sizes |
| Targets |
Fish Collagen Peptide targets multiple cellular processes. It inhibits the adipogenic differentiation of preadipocytes, suggesting effects on adipogenesis pathways. The peptide exhibits excellent biocompatibility, low antigenicity, and high direct cell adhesion ability, indicating interactions with cell adhesion molecules and extracellular matrix components. It participates in cell migration, differentiation, and proliferation. Its ability to trap red blood cells suggests hemostatic activity.
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| ln Vitro |
In vitro, Fish Collagen Peptide inhibits the adipogenic differentiation of preadipocytes. This suggests that the peptide can modulate adipogenesis in cell culture models. The peptide shows excellent biocompatibility and supports cell adhesion and proliferation. Its low molecular weight and high biological activity make it suitable for various cell-based assays. The peptide's ability to trap red blood cells suggests hemostatic properties in vitro.
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| ln Vivo |
In vivo, Fish Collagen Peptide ameliorates obesity in high fat diet-fed mice. This demonstrates its efficacy in a diet-induced obesity model. The peptide is used to make novel cardiac bioprosthetic valves that may have longer service life and less pain. Fish collagen combined with chitosan forms a hemostatic cotton that shows better hemostatic function than medical absorbent cotton. It is widely used in wound healing, tissue repair, and hemostasis.
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| Enzyme Assay |
The biological activity of Fish Collagen Peptide is assessed using various in vitro assays. For adipogenesis inhibition, preadipocytes (e.g., 3T3-L1) are cultured and differentiated in the presence of the peptide. Adipocyte differentiation is assessed by Oil Red O staining for lipid droplets and by measuring triglyceride content. Cell adhesion is assessed by seeding cells on peptide-coated surfaces and measuring attachment. Hemostatic activity is assessed using platelet aggregation or blood clotting assays.
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| Cell Assay |
For cellular studies, preadipocytes, fibroblasts, and other cell types are cultured in appropriate media. Cells are treated with Fish Collagen Peptide at various concentrations (e.g., 0.1-10 mg/mL) for 24-72 hours. Cell viability and proliferation are assessed using MTT or similar assays. Adipogenic differentiation is assessed by Oil Red O staining and qPCR for adipogenic markers (aP2, PPARγ). Cell migration is assessed using scratch wound healing assays. The peptide is typically dissolved in sterile water or PBS and filter-sterilized before use.
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| Animal Protocol |
In vivo efficacy studies for Fish Collagen Peptide are conducted in high fat diet-fed mouse models of obesity. Mice are fed a high-fat diet and treated with Fish Collagen Peptide via oral gavage or dietary supplementation. Body weight, food intake, and adipose tissue weights are measured. Glucose tolerance tests and insulin tolerance tests may be performed. Adipose tissue is harvested for histological analysis and gene expression studies. For wound healing studies, rodent wound models are used with topical or systemic peptide administration.
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| ADME/Pharmacokinetics |
Specific pharmacokinetic data for Fish Collagen Peptide are not typically reported due to its complex composition. The low molecular weight of the peptide facilitates absorption and utilization. The peptide is highly soluble and has excellent thermal stability. Pharmacokinetic studies would be required to determine the absorption and metabolism of specific peptide fractions. The peptide should be stored in a cool, dry place protected from light.
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| Toxicity/Toxicokinetics |
Fish Collagen Peptide has excellent biocompatibility, low antigenicity, and is considered safe for various applications. It is widely used in food and cosmetic products. In biomedical applications, it is used for wound dressing systems due to its biocompatibility and cell adhesion properties. No significant toxicity has been reported at the concentrations used for research and applications. However, as with any protein-based material, potential allergenicity should be considered.
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| Additional Infomation |
Fish Collagen Peptide is a marine-derived bioactive peptide that inhibits adipogenic differentiation and ameliorates obesity in high fat diet-fed mice. It has excellent biocompatibility, low antigenicity, and is used in wound healing, tissue repair, hemostasis, and cosmetics. No clinical trials exist. For research use only.
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| Molecular Formula |
C65H102N18O21
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|---|---|
| Molecular Weight |
1471.61339521408
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| Exact Mass |
300.179
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| CAS # |
9064-67-9
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| PubChem CID |
71571507
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
614.9±55.0 °C at 760 mmHg
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| Flash Point |
325.7±31.5 °C
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| Vapour Pressure |
0.0±3.8 mmHg at 25°C
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| Index of Refraction |
1.540
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| Source |
Tilapia fish skin
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| LogP |
-1.42
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| Hydrogen Bond Donor Count |
19
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| Hydrogen Bond Acceptor Count |
24
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| Rotatable Bond Count |
48
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| Heavy Atom Count |
104
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| Complexity |
3010
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| Defined Atom Stereocenter Count |
0
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| SMILES |
NCCCCC(NC(C1CCCN1C(CNC(C(NC(C(NC(CNC(C(NC(C(CC1C=CC=CC=1)NC(CNC(C(NC(C(C(CC)C)NC(CNC(C1CCCN1C(C(NC(CN)=O)CCC(=O)O)=O)=O)=O)=O)C)=O)=O)=O)CCCCN)=O)=O)CCC(=O)O)=O)CCC(=O)N)=O)=O)=O)C(=O)O
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| InChi Key |
WCTSASYOBIDUQP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C65H102N18O21/c1-4-36(2)55(81-51(88)34-72-61(99)45-18-13-29-83(45)64(102)42(22-25-54(92)93)76-48(85)31-68)63(101)74-37(3)56(94)70-32-50(87)77-44(30-38-14-6-5-7-15-38)60(98)78-39(16-8-10-26-66)57(95)71-33-49(86)75-41(21-24-53(90)91)59(97)79-40(20-23-47(69)84)58(96)73-35-52(89)82-28-12-19-46(82)62(100)80-43(65(103)104)17-9-11-27-67/h5-7,14-15,36-37,39-46,55H,4,8-13,16-35,66-68H2,1-3H3,(H2,69,84)(H,70,94)(H,71,95)(H,72,99)(H,73,96)(H,74,101)(H,75,86)(H,76,85)(H,77,87)(H,78,98)(H,79,97)(H,80,100)(H,81,88)(H,90,91)(H,92,93)(H,103,104)
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| Chemical Name |
6-amino-2-[[1-[2-[[5-amino-2-[[2-[[2-[[6-amino-2-[[2-[[2-[2-[[2-[[2-[[1-[2-[(2-aminoacetyl)amino]-4-carboxybutanoyl]pyrrolidine-2-carbonyl]amino]acetyl]amino]-3-methylpentanoyl]amino]propanoylamino]acetyl]amino]-3-phenylpropanoyl]amino]hexanoyl]amino]acetyl]amino]-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]acetyl]pyrrolidine-2-carbonyl]amino]hexanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6795 mL | 3.3976 mL | 6.7953 mL | |
| 5 mM | 0.1359 mL | 0.6795 mL | 1.3591 mL | |
| 10 mM | 0.0680 mL | 0.3398 mL | 0.6795 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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