| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Ferrozine does not have a biological target. It is a chemical reagent that functions as a chelating agent for ferrous iron (Fe²⁺). The compound selectively binds to Fe²⁺ ions to form a stable complex, which can be detected spectrophotometrically. This property makes it useful for the quantification of iron in various samples.
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|---|---|
| ln Vitro |
In vitro, Ferrozine is used as a spectrophotometric reagent for iron ions. It reacts with divalent iron to form a stable magenta complex species with an absorption peak at 562 nm. Its key analytical attributes include high molar absorptivity (~27,900–31,500 M⁻¹·cm⁻¹) and water solubility. It is used for the direct determination of iron in water and for the removal of iron from physiological fluids and tissue samples.
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| ln Vivo |
In vivo applications of Ferrozine are not typical, as it is primarily used in vitro as a colorimetric reagent. It is not administered to living organisms for therapeutic or diagnostic purposes. Its use is confined to laboratory settings for iron quantification in biological and environmental samples.
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| Enzyme Assay |
A cell-free assay for Ferrozine involves incubating the reagent with a sample containing ferrous iron. The resulting magenta complex is measured spectrophotometrically at 562 nm. The intensity of the color is proportional to the iron concentration, allowing for quantitative determination. The assay is simple, sensitive, and does not require organic extraction steps.
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| Cell Assay |
Cellular experiments with Ferrozine are not typical, as it is used as a biochemical reagent in cell-free assays for iron quantification rather than in cell-based studies. Its primary application is in the analysis of iron levels in biological fluids and tissue homogenates.
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| Animal Protocol |
In vivo animal experiments for Ferrozine are not applicable, as it is a colorimetric reagent used in vitro.
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| ADME/Pharmacokinetics |
Ferrozine has a molecular formula of C20H12N4O6S2-2 and a molecular weight of 468.5 g/mol. Its CAS number is 69898-45-9. Specific pharmacokinetic properties are not applicable as it is not a therapeutic drug. It is used in vitro as a research reagent. For storage, it should be kept in a cool, dry place.
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| Toxicity/Toxicokinetics |
Toxicity data for Ferrozine are not provided in the available sources. As a chemical reagent, it should be handled with appropriate safety precautions. It is intended for research use only and is not for human consumption.
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| References | |
| Additional Infomation |
ferrous complex forms a stable magenta solution with ferrous ions. This complex exhibits an absorption peak at 562 nm and can be used as a reagent and indicator for iron.
Ferrozine (CAS: 69898-45-9) is a chromogenic chelator for ferrous iron. Its synonyms include 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt. It is used as a spectrophotometric reagent for the quantification of iron in clinical chemistry, environmental analysis, and biological research. It is not a therapeutic drug and is strictly for research purposes. |
| Molecular Formula |
C20H13N4NAO6S2
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|---|---|
| Molecular Weight |
492.4602
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| Exact Mass |
492.017
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| CAS # |
69898-45-9
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| Related CAS # |
69898-45-9 (mono-hydrochloride salt)
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| PubChem CID |
34127
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| Appearance |
Light yellow to yellow solid powder
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| Melting Point |
≥300 °C
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| LogP |
4.58
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
34
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| Complexity |
801
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=NC(=C1)C2=NC(=C(N=N2)C3=CC=C(C=C3)S(=O)(=O)[O-])C4=CC=C(C=C4)S(=O)(=O)[O-].[Na+].[Na+]
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| InChi Key |
DDIHLFCSEHAOJZ-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C20H14N4O6S2.2Na/c25-31(26,27)15-8-4-13(5-9-15)18-19(14-6-10-16(11-7-14)32(28,29)30)23-24-20(22-18)17-3-1-2-12-21-17;;/h1-12H,(H,25,26,27)(H,28,29,30);;/q;2*+1/p-2
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| Chemical Name |
disodium;4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~101.53 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0306 mL | 10.1531 mL | 20.3062 mL | |
| 5 mM | 0.4061 mL | 2.0306 mL | 4.0612 mL | |
| 10 mM | 0.2031 mL | 1.0153 mL | 2.0306 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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