| Size | Price | |
|---|---|---|
| 500mg |
| Targets |
The primary target of Euchrysine 3RX is nucleic acids, particularly DNA and RNA. As an acridine dye, it intercalates between the base pairs of DNA, which can inhibit DNA replication and transcription. This intercalation is the basis for its use as a fluorescent probe and its potential antimicrobial and anticancer activities.
|
|---|---|
| ln Vitro |
Advice (The protocol that follows is our suggested one; it should be adjusted to meet your unique requirements as it simply offers guidance) [4]. 1. Apply acridine orange zinc chloride salt (1 μM; 20 minutes; 37°C) to the cells to stain them. 2. Use PBS to clean the cells. 3. Use a confocal laser scanning microscope (Olympus FV3000) to observe cells.
In vitro, Euchrysine 3RX has been shown to intercalate with DNA and RNA, resulting in fluorescence enhancement. It has been used as a stain for nucleic acids in various biological applications. The compound has also been studied for its antimicrobial and anticancer activities, although specific details are limited. |
| ln Vivo |
Over the course of 30 days, ciprodope orange (0.1 mg/kg, intravenous, zinc chloride salt) did not exhibit any clinical toxicity, and there were no abnormalities detected in the blood [1].
In vivo, Euchrysine 3RX is not used as a therapeutic agent. Its primary use is as a research tool and as a biological stain. It has been used in various staining applications in histology and cytology. However, specific in vivo efficacy data are not available. |
| Enzyme Assay |
In vitro enzyme or receptor binding assays are not applicable for Euchrysine 3RX, as it is not a specific enzyme inhibitor or receptor ligand. Its interaction with nucleic acids can be studied using spectroscopic methods, such as fluorescence spectroscopy and UV-Vis spectroscopy. The intercalation of the compound with DNA can be assessed by measuring changes in fluorescence or absorbance.
|
| Cell Assay |
In vitro cell-based assays for Euchrysine 3RX are performed using various cell lines to study its cytotoxic effects. Cells are treated with the compound, and cell viability and proliferation are assessed. The compound's effects on DNA replication and transcription are evaluated. Its antimicrobial activity is assessed using standard microbiological methods.
|
| Animal Protocol |
In vivo animal experiments for Euchrysine 3RX are limited, as it is primarily used as a research tool. Its use as a stain in histology and cytology does not involve systemic administration. The compound is not used as a therapeutic agent.
|
| ADME/Pharmacokinetics |
Pharmacokinetic (PK) properties of Euchrysine 3RX are not well-characterized. The compound has a molecular weight of 265.74 and a molecular formula of C14H14ClN3. It is a solid at room temperature. The compound is soluble in water and alcohol. It is typically stored at room temperature.
|
| Toxicity/Toxicokinetics |
Toxicology (toxicology) data for Euchrysine 3RX indicate that it is an irritant and should be handled with appropriate safety precautions. It may cause irritation to the skin, eyes, and respiratory tract. The compound is for research use only and not for human therapeutic use.
|
| References |
|
| Additional Infomation |
A cationic cytochemical staining agent specifically targeting the cell nucleus, particularly DNA. It can be used as a live staining agent and a fluorescent cytochemical staining agent. It may cause mutations in microorganisms.
Other information: Euchrysine 3RX is an acridine dye used as a biological stain and fluorescent probe. It is also known as 3,6-diamino-10-methylacridinium chloride. The compound intercalates with nucleic acids. Its CAS number is 10127-02-3. |
| Molecular Formula |
C17H20CL3N3ZN
|
|---|---|
| Molecular Weight |
438.1288
|
| Exact Mass |
736.135
|
| CAS # |
10127-02-3
|
| Related CAS # |
Acridine Orange hydrochloride;65-61-2;Acridine Orange base;494-38-2
|
| PubChem CID |
159263
|
| Appearance |
Brown to reddish brown solid powder
|
| Boiling Point |
468.6ºC at 760 mmHg
|
| Melting Point |
165 °C
|
| Flash Point |
237.2ºC
|
| LogP |
10.02
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
24
|
| Complexity |
298
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
VADJQOXWNSPOQA-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C17H19N3.3ClH.Zn/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;/h5-11H,1-4H3;3*1H;/q;;;;+2/p-2
|
| Chemical Name |
zinc;3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine;dichloride;hydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2824 mL | 11.4121 mL | 22.8243 mL | |
| 5 mM | 0.4565 mL | 2.2824 mL | 4.5649 mL | |
| 10 mM | 0.2282 mL | 1.1412 mL | 2.2824 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
