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| Targets |
Ethylenediaminetetraacetic acid sodium hydrate targets metal ions, specifically divalent and trivalent metal cations including calcium. As a chelator, it forms stable complexes with metal ions, sequestering them and preventing their participation in biological or chemical reactions. This chelation activity is the basis for its antihypercalcaemic and anticoagulant effects. By binding to metal ions, EDTA sodium hydrate can reduce oxidative damage to proteins catalyzed by metal ions.
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| ln Vitro |
In vitro, Ethylenediaminetetraacetic acid sodium hydrate is used as a metal chelator in various biochemical applications. It binds to divalent and trivalent metal cations including calcium. The compound is used to reduce oxidative damage to proteins catalyzed by metal ions and to maintain a reducing environment. It is commonly used in laboratory and industrial applications as a chelating agent. EDTA is used in buffer formulations to chelate metal ions that could interfere with enzymatic reactions or cause protein degradation.
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| ln Vivo |
In vivo, Ethylenediaminetetraacetic acid sodium hydrate has antihypercalcaemic and anticoagulant activity. It can be used to chelate calcium in the blood, reducing calcium levels. The compound's anticoagulant activity is due to its ability to chelate calcium, which is required for the coagulation cascade. EDTA sodium hydrate is not typically used as a therapeutic agent in humans but has applications in research and as an anticoagulant for blood collection.
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| Enzyme Assay |
In vitro experiments with Ethylenediaminetetraacetic acid sodium hydrate typically involve preparing solutions in water or buffer at concentrations ranging from 0.1-10 mM. The compound is used to chelate metal ions in reaction mixtures or buffer solutions. For protein protection studies, EDTA is added to protein solutions to prevent metal-catalyzed oxidation. For anticoagulation studies, EDTA is added to blood samples to prevent clotting. The compound is soluble in water and is typically stored at room temperature.
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| Cell Assay |
In vitro cell-based assays using Ethylenediaminetetraacetic acid sodium hydrate are performed to study the effects of metal ion chelation on cellular processes. Cells are treated with EDTA at concentrations of 0.1-5 mM to chelate extracellular metal ions. The compound's effects on cell viability, proliferation, and signaling pathways can be assessed. EDTA can be used to study the role of calcium and other metal ions in cellular processes. The compound is typically dissolved in water or cell culture medium for treatment.
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| Animal Protocol |
In vivo animal experiments with Ethylenediaminetetraacetic acid sodium hydrate are not typical as the compound is primarily used as a chelating agent in vitro. However, EDTA has been used in animal studies to chelate metal ions and study their physiological roles. The compound can be administered intravenously or intraperitoneally to study its effects on metal ion homeostasis. Dosing would depend on the specific experimental design. EDTA is not typically used as a therapeutic agent in animals.
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| ADME/Pharmacokinetics |
Ethylenediaminetetraacetic acid sodium hydrate has a molecular weight of 416.20 g/mol and the formula C10H16N2Na4O10. The compound appears as a white to off-white solid. For storage, the compound is kept at -20°C or at room temperature for up to 3 years. It is soluble in water. Its purity is typically ≥95%. The compound is also known as EDTA sodium hydrate. EDTA is a widely used chelating agent in laboratory and industrial applications.
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| Toxicity/Toxicokinetics |
Specific toxicological data for Ethylenediaminetetraacetic acid sodium hydrate are not extensively provided. As a chelating agent, it can cause electrolyte imbalances by chelating calcium and other essential metal ions. The compound is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves and eye protection. The compound may cause irritation to skin, eyes, and respiratory tract upon contact.
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| References |
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| Additional Infomation |
Ethylenediaminetetraacetic acid sodium hydrate (CAS 10378-23-1) is a metal chelator that binds to divalent and trivalent metal cations including calcium. It has the molecular formula C10H16N2Na4O10 and a molecular weight of 416.20 g/mol. The compound has antihypercalcaemic and anticoagulant activity. EDTA sodium hydrate can reduce oxidative damage to proteins catalyzed by metal ions and maintain a reducing environment. It is commonly used in laboratories and industrial applications as a chelating agent. The compound is also known as EDTA sodium hydrate. It is intended for research use only.
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| Molecular Formula |
C10H16N2NA4O10
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| Molecular Weight |
416.2005
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| Exact Mass |
416.039
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| CAS # |
10378-23-1
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| Related CAS # |
Ethylenediaminetetraacetic acid;60-00-4
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| PubChem CID |
21917311
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| Appearance |
White to off-white solid powder
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| Boiling Point |
614.2ºC at 760mmHg
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| Melting Point |
>300ºC
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| Flash Point |
325.2ºC
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
26
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| Complexity |
293
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
KSYNLCYTMRMCGG-UHFFFAOYSA-J
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| InChi Code |
InChI=1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4
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| Chemical Name |
tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate;dihydrate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4027 mL | 12.0135 mL | 24.0269 mL | |
| 5 mM | 0.4805 mL | 2.4027 mL | 4.8054 mL | |
| 10 mM | 0.2403 mL | 1.2013 mL | 2.4027 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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