| Size | Price | |
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| Other Sizes |
| Targets |
Ethyl Violet is a dye and does not have a specific biological target. It stains biological tissues by binding to negatively charged molecules like nucleic acids and proteins through ionic and hydrophobic interactions. It is used as a pH indicator, changing from yellow at pH 0.0 to blue at pH 3.5.
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|---|---|
| ln Vitro |
Ethyl Violet is used in vitro as a staining reagent. It is employed in histology to stain pancreatic tissue and elastin. It is also used in microbiology for the detection of gram-negative bacteria. In analytical chemistry, it is used for the extractive-spectrophotometric determination of anionic surfactants.
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| ln Vivo |
Ethyl Violet is not used in vivo for therapeutic or diagnostic purposes. Its applications are limited to in vitro staining and analytical chemistry.
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| Enzyme Assay |
For staining, tissue sections or bacterial smears are incubated with an Ethyl Violet solution. The stain binds to the target structures, providing color contrast for microscopic examination. For analytical applications, it is used in a liquid-liquid extraction followed by spectrophotometric measurement.
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| Cell Assay |
In cell-based assays, Ethyl Violet is used as a staining agent. Cells are fixed and then incubated with the dye. The stained cells can be observed under a microscope to visualize cellular structures. It is also used in viability staining in some contexts.
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| Animal Protocol |
Ethyl Violet is not used in in vivo animal experiments. Its use is restricted to in vitro applications in histology, microbiology, and analytical chemistry.
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| ADME/Pharmacokinetics |
Ethyl Violet has a molecular weight of 492.14 and the chemical formula C31H42ClN3. It is a powder with an olive green color. It is soluble in water (10 mg/mL, very deep purple). The pH range is 0.0 (yellow) to 3.5 (blue). The Color Index number is 42600.
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| Toxicity/Toxicokinetics |
Ethyl Violet is considered a non-carcinogenic alternative to Crystal Violet. However, as a dye, it may cause staining of skin and clothing. Standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
Ethyl violet is an organochloride salt, a monochloride salt of the ethyl violet cation. It is commonly used in solutions of iron-resorcinol-fuchsin mixed dyes to visualize elastic fibers. It can also be used as a histological dye and a fluorescent dye. Its molecular structure contains the ethyl violet cation.
Ethyl Violet is also known as Basic Violet 4, C.I. 42600. It is a cationic triarylmethane dye. It is used as a reagent and stain in various research and industrial applications. |
| Molecular Formula |
C31H42CLN3
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|---|---|
| Molecular Weight |
492.1383
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| Exact Mass |
491.306
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| CAS # |
2390-59-2
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| PubChem CID |
16955
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| Appearance |
Green to dark green solid powder
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| Density |
0.9659 (rough estimate)
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| Boiling Point |
631.13°C (rough estimate)
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| Melting Point |
>250°C
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| Index of Refraction |
1.6010 (estimate)
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| LogP |
3.804
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
35
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| Complexity |
615
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JVICFMRAVNKDOE-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C31H42N3.ClH/c1-7-32(8-2)28-19-13-25(14-20-28)31(26-15-21-29(22-16-26)33(9-3)10-4)27-17-23-30(24-18-27)34(11-5)12-6;/h13-24H,7-12H2,1-6H3;1H/q+1;/p-1
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| Chemical Name |
[4-[bis[4-(diethylamino)phenyl]methylidene]cyclohexa-2,5-dien-1-ylidene]-diethylazanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~5 mg/mL (~10.16 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0319 mL | 10.1597 mL | 20.3194 mL | |
| 5 mM | 0.4064 mL | 2.0319 mL | 4.0639 mL | |
| 10 mM | 0.2032 mL | 1.0160 mL | 2.0319 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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