| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
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| ln Vitro |
Ingredients for the EthD-1 Working Solution and Methods of Use 1.1 Make a stock solution of EthD-1 by mixing 2 mM DMSO with it. Note: It is advised to aliquot the EthD-1 storage solution and keep it in the dark at -20°C or -80°C. 1.2 Components of the working solution: To create a 0.1–10 μM EthD-1 working solution, mix the storage solution with either PBS or previously prepared serum-free cell culture media. Note: Please prepare the EthD-1 working solution for immediate usage by adjusting its concentration to suit the current circumstances. Staining 2.1 Cells Centrifugation is used to gather the cells, then PBS is added and the cells are washed twice for five minutes each time. Adherent cells: take out the trash cells and put in the digested islet cells. Add PBS and wash twice for five minutes after centrifugation and discarding the supernatant. Note: Slide staining can be used to stain adhering cells in the absence of flow cytometry investigations. 2.2 For 15 to 60 minutes, add 1 mL of the EthD-1 working solution and the stainless steel working solution. 2.3 Centrifuge for 3–4 seconds at 4°C at 400 g. 2.4 After adding PBS, wash the cells twice for five minutes each. 2.5 Re-suspend the cells in 1 milliliter of PBS or serum-free water, and examine using a flow cytometer or fluorescence microscope. Observations 1. After aliquoting, it is advised to store the EthD-1 storage solution in the dark at -20°C or -80°C to prevent recurrent freezing and thawing. It can be kept for one month at -20°C and for six months at -80°C. 2. Please modify the EthD-1 working fluid concentration based on the current circumstances. 3. This product may not be used for clinical diagnosis or treatment, nor may it be included into food or medication. It is intended solely for professional use in scientific study. 4. Please wear disposable gloves and a lab coat for your health and safety.
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| References |
| Molecular Formula |
C46H50CL4N8
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|---|---|
| Molecular Weight |
856.76
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| Exact Mass |
928.235
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| CAS # |
61926-22-5
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| Related CAS # |
61926-22-5 (HCl);68687-01-4 (cation);
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| PubChem CID |
12328896
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| Appearance |
Red to reddish brown solid powder
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| Melting Point |
≥250ºC(lit.)
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| Flash Point |
85ºC
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
13
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| Heavy Atom Count |
56
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| Complexity |
1040
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
BGWLYQZDNFIFRX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C46H46N8.2ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;2*1H
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| Chemical Name |
5-[3-[2-[3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propylamino]ethylamino]propyl]-6-phenylphenanthridin-5-ium-3,8-diamine;dichloride
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| Synonyms |
EthD 1; EthD-1; Ethidium Homodimer-1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1672 mL | 5.8359 mL | 11.6719 mL | |
| 5 mM | 0.2334 mL | 1.1672 mL | 2.3344 mL | |
| 10 mM | 0.1167 mL | 0.5836 mL | 1.1672 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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