| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
ER
|
|---|---|
| ln Vitro |
Human endometrial stem cells (hEnSC) are exposed to estradiol hemihydrate (10 nM) for seven days, during which time it promotes neurite branching and neural development [1]. In hEnSC-differentiated neuronal-like cells, estradiol hemihydrate (17β-estradiol, 10 nM, 7 days) enhances the expression of neuron-like cell markers (Tuj-1, nestin, and NF-H) [1]. Assay for cell differentiation [1]
|
| ln Vivo |
Estradiol hemihydrate (1 nM, hippocampal slices from FBN-ARO-KO mice) recovers long-term potentiation (LTP) amplitude [1]. Estradiol hemihydrate (0.0167 mg, subcutaneously implanted in FBN-ARO-KO mice) cures molecular and functional abnormalities in FBN-ARO-KO animals [1].
|
| Cell Assay |
Cell differentiation assay[1]
Cell Types: Human endometrial stem cells (hEnSC) isolated from human endometrial tissue Tested Concentrations: 10 nM Incubation Duration: 7 days Experimental Results: Neural markers (Tuj-1, Nestin and NF The percentage increases of -H)-positive cells were 62.2±1.3%, 71.5±4% and 51.2±1.5%, respectively. Immunofluorescence[1] Cell Types: Human endometrial stem cells (hEnSC) isolated from human endometrial tissue Tested Concentrations: 10 nM Incubation Duration: 7 days Experimental Results: Neural markers (Tuj-1, Nestin and NF- H) The percentage increases of positive cells were 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively. |
| Animal Protocol |
Animal/Disease Models: FBN-ARO-KO mice [2]
Doses: 1 nM Route of Administration: Treatment of hippocampal slices Experimental Results: Long-term potentiation (LTP) amplitude was restored in both male and female mice. Animal/Disease Models: FBN-ARO-KO mice [2] Doses: 0.0167 mg Route of Administration: Alzet minipump containing estradiol (subcutaneously (sc) (sc) implanted) was used, and examinations were performed 7 days after minipump implantation. Experimental Results: E2 levels in the hippocampus and cortex were restored to 93%, AKT, ERK and CREB phosphorylation in the hippocampus and cortex were restored to 90-95%, BDNF levels were restored to 80-90%, and forebrain synaptophysin and PSD95 were restored. Rescues spatial learning and memory deficits. |
| References | |
| Additional Infomation |
Estradiol hemihydrate is the hemihydrate form of estradiol, the most potent natural estrogen in the body. Estradiol hemihydrate diffuses across cell membranes and binds to nuclear estrogen receptors located in the reproductive tract, mammary glands, pituitary gland, hypothalamus, liver, and bones, thereby activating these receptors. The activated complex binds to estrogen-responsive elements on DNA, activating the transcription of genes involved in female reproductive system function and secondary sexual characteristic development. The 17β-isomer of estradiol is an aromatic C18 steroid with hydroxyl groups at both the 3β and 17β positions. Estradiol 17β is the most potent estrogenic steroid in mammals. See also: Estradiol (including the active moiety)... See more...
|
| Molecular Formula |
2[C18H24O2].H2O
|
|---|---|
| Exact Mass |
562.365
|
| CAS # |
35380-71-3
|
| Related CAS # |
Alpha-Estradiol;57-91-0;Estradiol (Standard);50-28-2;Estradiol-d3;79037-37-9;Estradiol-d4;66789-03-5;Estradiol-d5;221093-45-4;Estradiol-13C2;82938-05-4;Estradiol (cypionate);313-06-4;Estradiol benzoate;50-50-0;Estradiol enanthate;4956-37-0;Estradiol hemihydrate;35380-71-3;Estradiol-d2;53866-33-4;Estradiol-13C6;Estradiol-d2-1;3188-46-3;rel-Estradiol-13C6; 979-32-8 (valerate); 113-38-2 (dipropionate); 57-63-6 (ethinyl); 172377-52-5 (sulfamate); 3571-53-7 (undecylate)
|
| PubChem CID |
154274
|
| Appearance |
White to off-white solid
|
| Boiling Point |
445.9ºC at 760mmHg
|
| Flash Point |
209.6ºC
|
| Source |
Endogenously produced metabolite
|
| LogP |
3.544
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
41
|
| Complexity |
382
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
O.OC1C=CC2[C@H]3CC[C@@]4([C@H](CC[C@H]4[C@@H]3CCC=2C=1)O)C.OC1C=CC2[C@H]3CC[C@@]4([C@H](CC[C@H]4[C@@H]3CCC=2C=1)O)C
|
| InChi Key |
ZVVGLAMWAQMPDR-WVEWYJOQSA-N
|
| InChi Code |
InChI=1S/2C18H24O2.H2O/c2*1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20;/h2*3,5,10,14-17,19-20H,2,4,6-9H2,1H3;1H2/t2*14-,15-,16+,17+,18+;/m11./s1
|
| Chemical Name |
(8R,9S,13S,14S,17S)-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol;hydrate
|
| Synonyms |
ESTRADIOL HEMIHYDRATE; 35380-71-3; beta-Estradiol semihydrate; UNII-CXY7B3Q98Z; CXY7B3Q98Z; Estra-1,3,5(10)-triene-3,17beta-diol hydrate (2:1); beta-Estradiol hemihydrate; Estrasorb (TN);
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (~355.4 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.88 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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