| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg | |||
| Other Sizes |
| Targets |
Estradiol-d4 targets the Estrogen Receptors (ERalpha and ERbeta). As an internal standard, it is chemically identical to the natural analyte (estradiol) and interacts with these receptors in the same way. However, it is not used for pharmacological purposes but as a surrogate to correct for matrix effects during analytical method development.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a stable isotope-labeled internal standard, Estradiol-d4 is chemically inert and exhibits identical extraction recovery and chromatographic behavior to the unlabeled estradiol. It does not exhibit pharmacological activity in vitro and is used solely as an internal standard, not as a biological effector in enzyme or receptor assays. |
| ln Vivo |
Estradiol-d4 is not used in cell-based activity assays. Its application is strictly in analytical chemistry. It is added to cell culture media or lysates at a known concentration to serve as an internal standard for sample preparation and data normalization when quantifying endogenous estradiol production or metabolism by LC-MS.
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| Enzyme Assay |
Estradiol-d4 is not used in cell-free enzyme or receptor binding assays. Its application is in analytical chemistry. The typical protocol for its use as an internal standard involves spiking a known amount of Estradiol-d4 into biological samples (e.g., plasma, tissue homogenates) before sample extraction and purification. The ratio of the analyte to the internal standard is then used for LC-MS/MS quantification.
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| Cell Assay |
Estradiol-d4 is not used in pharmacological cell-based assays. Instead, it is a critical component of analytical protocols for measuring estradiol levels. It is added to biological samples (from in vivo or in vitro studies) before processing. The concentration of estradiol is determined by comparing its mass spectral signal to that of the known amount of Estradiol-d4 internal standard.
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| Animal Protocol |
Estradiol-d4 is used as a standard in animal studies. A known amount of the compound is spiked into collected plasma or tissue samples before processing. It is used solely for analytical correction to accurately measure endogenous estradiol levels in pharmacokinetic (PK) and pharmacodynamic (PD) studies, not as a therapeutic agent.
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| ADME/Pharmacokinetics |
As a stable isotope-labeled standard, the pharmacokinetics of Estradiol-d4 are identical to the native analyte, estradiol. It is absorbed, distributed, metabolized, and excreted (ADME) via the same pathways, making it a perfect tracer for studying estradiol's disposition in biological systems.
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| Toxicity/Toxicokinetics |
Estradiol-d4 has no pharmacological toxicity. Safety concerns are limited to the hazards associated with handling solvents (e.g., methanol) in which the compound is often supplied and standard precautions for handling laboratory chemicals.
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| References |
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| Additional Infomation |
Estradiol-d4 is a crucial analytical standard for the accurate diagnosis and monitoring of conditions related to estrogen imbalance, such as menopause, infertility, and estrogen-sensitive cancers. Its use in LC-MS/MS provides superior specificity and sensitivity over older immunoassay methods.
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| Molecular Formula |
C18H20D4O2
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|---|---|
| Molecular Weight |
276.41
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| Exact Mass |
276.203
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| CAS # |
66789-03-5
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| Related CAS # |
Estradiol;50-28-2;Estradiol-d3;79037-37-9;Estradiol-d5;221093-45-4;rel-Estradiol-13C6
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| PubChem CID |
87229045
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| Appearance |
White to off-white solid powder
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| LogP |
3.609
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
20
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| Complexity |
382
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| Defined Atom Stereocenter Count |
5
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| SMILES |
[2H]C1=CC2=C(CC[C@@H]3[C@@H]2CC[C@]4([C@H]3CC([C@@H]4O)([2H])[2H])C)C(=C1O)[2H]
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| InChi Key |
VOXZDWNPVJITMN-GKCQEISNSA-N
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| InChi Code |
InChI=1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1/i3D,7D2,10D
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| Chemical Name |
(8R,9S,13S,14S,17S)-2,4,16,16-tetradeuterio-13-methyl-7,8,9,11,12,14,15,17-octahydro-6H-cyclopenta[a]phenanthrene-3,17-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (904.45 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.53 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.53 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.53 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6178 mL | 18.0891 mL | 36.1781 mL | |
| 5 mM | 0.7236 mL | 3.6178 mL | 7.2356 mL | |
| 10 mM | 0.3618 mL | 1.8089 mL | 3.6178 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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