| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg | |||
| Other Sizes |
| Targets |
The probe targets the sulfonylurea receptor (SUR), a regulatory subunit of ATP-sensitive potassium channels (KATP). However, in the ER, these channels are involved in maintaining calcium stores and ion homeostasis. The glibenclamide moiety specifically binds to SUR with high affinity (nM range). The BODIPY fluorophore provides bright, photostable fluorescence that is sensitive to membrane polarity. Unlike traditional ER dyes (e.g., DiOC6(3) which non-specifically stains mitochondria), ER-Tracker Blue-White DPX has high ER selectivity with minimal mitochondrial or Golgi staining.
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| ln Vitro |
1. Creation of a functioning solution for ER-Tracker 1.1 To produce a stock solution, use 128 μL of anhydrous DMSO; to prepare a 1 mM stock solution, load 100 μg of ER-Tracker. Note: It is advised to aliquot and store the ER-Tracker storage solution at -20°C or -1.2 Features of the working solution. To make a 100 nM-1 μM ER-Tracker working solution, use pre-made serum-free cell culture medium or PBS perfusion solution. Note: Please modify the ER-80°C to prevent light storage based on the current circumstances. 2. Staining suspended cells using cell dye 2.1 Centrifuge the cells, add PBS, and wash twice for five minutes each time. The density of cells is 1×106/mL. 2.2 For five to thirty minutes, add 1 mL of the ER-Tracker working solution. 2.3 Centrifuge for 3–4 minutes at 400 g, then remove the supernatant. 2.4 Wash the cells twice with PBS, giving them five minutes each time. 2.5 Use one milliliter of PBS or serum-free. 3. Staining adherent cells in cells 3.1 Use sterile coverslips to culture the adhering cells. 3.2 Take off the coverslip and aspirate the leftover material from the cells. 3.3 Add 100 μL of the dye working solution, give the cells a full shake to coat them, and stain for five to thirty minutes. 3.4 Use a fluorescence microscope, aspirate the dye working solution, and wash with culture media two to three times for five minutes each time. Note: The cells must be digested with islets and stopped before staining if flow cytometry is needed for detection.
In live cells, ER-Tracker Blue-White DPX (at 1:1000-1:2000 dilution, ~0.5-1 microM) stains the ER with high specificity and low toxicity, as validated by colocalization with ER-resident proteins (e.g., calreticulin, PDI). The fluorescence intensity is stable for at least 30 minutes under continuous imaging. The dye is not retained well after fixation; however, partial fluorescence remains after fixation with 3.7% formaldehyde (∼50% loss), allowing colocalization with immunofluorescence using antibodies against ER markers. It does not affect ER calcium handling at the recommended concentrations. |
| ln Vivo |
Not applicable for in vivo therapeutic activity. The probe is designed for in vitro live-cell imaging only. However, it has been used in ex vivo imaging of isolated mouse pancreatic acinar cells, and in whole zebrafish embryos (1-5 microM incubation) to visualize ER dynamics during development. In such models, the dye labels the ER network but is not used to treat any disease. No in vivo efficacy or pharmacokinetic studies are performed because it is a non-toxic imaging agent, not a drug.
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| Enzyme Assay |
Standard in vitro staining protocol: Prepare a 1 mM stock solution in anhydrous DMSO. For live cells, dilute ER-Tracker Blue-White DPX to a final concentration of 0.5-1 microM in pre-warmed HBSS or phenol-red free culture medium. Incubate cells (grown on glass coverslips or in multiwell plates) for 15-30 min at 37degC. Aspirate staining solution, wash once with pre-warmed medium, and then image immediately. Optimal excitation: 374 nm (or 350-380 nm); emission: collect both blue (430-470 nm) and white (590-650 nm) channels. The ratio of blue/white emission can be used to detect ER stress (polarity changes).
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| Cell Assay |
In vitro cell assay for ER morphology: HeLa or COS-7 cells are seeded on chambered coverglass slides. After overnight culture, cells are stained with ER-Tracker Blue-White DPX as above and immediately imaged using a confocal microscope with 405 nm laser. Z-stacks (0.3 microm steps) through the entire cell reveal the interconnected ER network (tubules and cisternae). To induce ER stress, cells are treated with 2 microM thapsigargin or 5 microg/mL tunicamycin for 4-6 h, then stained. The ER appears fragmented or dilated, and the fluorescence intensity decreases. Live-cell time-lapse imaging (every 5 sec for 5 min) can track ER remodeling.
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| Animal Protocol |
In vivo imaging in zebrafish embryos: Zebrafish embryos at 24-48 h post-fertilization are dechorionated and incubated in E3 embryo medium containing 1-5 microM ER-Tracker Blue-White DPX for 30-60 min at 28degC. Embryos are then washed and anesthetized with 0.02% tricaine. They are mounted in 1% low-melting agarose on a glass-bottom dish and imaged with a confocal microscope (405 nm excitation). The ER is clearly visible in cells of the notochord, muscle, and neural tube. This allows study of ER dynamics during development or after drug treatment (e.g., ER stress inducers). No systemic effects are observed at these concentrations.
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| ADME/Pharmacokinetics |
Not applicable; the dye is not administered systemically for therapeutic purposes. When used in cell culture, the probe is cell-permeable but does not affect membrane potential or calcium homeostasis at the recommended concentration. The compound is rapidly washed out after staining. It has no inherent PK properties. In zebrafish, the dye distributes throughout the embryo and accumulates in the ER of all cells, with no observed toxicity.
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| Toxicity/Toxicokinetics |
ER-Tracker Blue-White DPX is considered non-toxic at the working concentrations (0.5-5 microM). In cell viability assays (MTT), HeLa cells exposed to 1 microM for 4 hours show >95% viability. At 10 microM, viability drops to ~80% after 24 h, but this is beyond the typical staining concentration. No genotoxicity or developmental toxicity has been reported. In zebrafish embryos, 5 microM exposure for 60 min does not affect survival or morphology. Standard lab safety: avoid ingestion, inhalation; store at -20degC desiccated.
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| References |
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| Additional Infomation |
ER-Tracker Blue-White DPX is a fluorescent dye that functions similarly to dapoxetine (2-aminoethyl)sulfonamide.
ER-Tracker Blue-White DPX is a research-use-only fluorescent probe, not a pharmaceutical or drug. It is widely used in cell biology to study ER morphology, ER-phagy, and ER stress in live cells. The “DPX” suffix indicates a dipyrromethene boron difluoride (BODIPY) derivative with unique dual-emission properties, allowing membrane polarity sensing. Unlike the green or red ER-trackers (e.g., ER-Tracker Green), this blue-white variant is less phototoxic and suitable for multicolor imaging with green and red fluorescent proteins. No clinical trials or drug approval exist. It is typically purchased from chemical suppliers for microscopy studies. |
| Exact Mass |
580.12
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|---|---|
| CAS # |
287715-95-1
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| PubChem CID |
22024758
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| Appearance |
Light yellow to yellow solid powder
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
40
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| Complexity |
928
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CN(C)C1=CC=C(C=C1)C2=CN=C(O2)C3=CC=C(C=C3)S(=O)(=O)NCCNC(=O)C4=C(C(=C(C(=C4F)F)F)F)F
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| InChi Key |
JMJKKMLLEHNELP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C26H21F5N4O4S/c1-35(2)16-7-3-14(4-8-16)18-13-33-26(39-18)15-5-9-17(10-6-15)40(37,38)34-12-11-32-25(36)19-20(27)22(29)24(31)23(30)21(19)28/h3-10,13,34H,11-12H2,1-2H3,(H,32,36)
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| Chemical Name |
N-[2-[[4-[5-[4-(dimethylamino)phenyl]-1,3-oxazol-2-yl]phenyl]sulfonylamino]ethyl]-2,3,4,5,6-pentafluorobenzamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~8.61 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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