| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg | |||
| Other Sizes |
| ln Vitro |
Antitumor Activity in Potato Disc Bioassay: Epifriedelanol, isolated from Vitis trifolia, demonstrated significant antitumor activity in the potato disc bioassay. At concentrations of 40 μg/disc and 60 μg/disc, it showed 100% inhibition of crown gall tumor formation induced by Agrobacterium tumefaciens on potato discs. This activity was compared to vincristine, which showed similar inhibition at 6.25 μg/disc [1]
. - Cytotoxicity Against Human Tumor Cells: The study notes that epifriedelanol was previously tested against five human tumor cell lines at a concentration of 10 μg/mL, but no cytotoxicity was observed in those tests [1] . |
|---|---|
| Cell Assay |
Potato Disc Bioassay for Antitumor Activity: The antitumor activity of epifriedelanol was evaluated using the potato disc bioassay. In this assay, discs are cut from surface-sterilized potato tubers. The test compound (epifriedelanol), dissolved in an appropriate solvent, is applied to the discs along with a suspension of Agrobacterium tumefaciens. The discs are then incubated for a period to allow for tumor formation. After incubation, the number of crown gall tumors formed on the discs is counted. The antitumor activity is determined by the percentage inhibition of tumor formation compared to a negative control (solvent only) and a positive control (e.g., vincristine). In this study, epifriedelanol at 40 and 60 μg/disc resulted in 100% inhibition of tumor formation [1]
. |
| References | |
| Additional Infomation |
Epi-Friedelanol is a triterpenoid compound. It has been reported in Elephantopus scaber, Anchietea salutaris, and other organisms with available data. See also: Friedelinol (note moved to).
Source and Isolation: Epifriedelanol (friedelan-3β-ol) was isolated from the whole plant of Vitis trifolia L. (Vitaceae), commonly known as "Amallata" in Bangladesh. The plant was collected from Dhaka in October 1997. The isolation yield was 0.0055% [1] . - Traditional Uses of the Source Plant: The roots of Vitis trifolia are used in traditional medicine for various ailments, including inflammation of the spleen, liver complaints, blood purification, biliousness, heart troubles, and also as a tonic, stomachic, and expectorant [1] . - Structural Data: The study provides comprehensive ¹H-NMR (500 MHz), ¹³C-NMR (125 MHz), and FABMS data for epifriedelanol, noting that such detailed NMR spectral data had not been previously reported despite the compound being widespread in plants. The FABMS showed an [M+H]⁺ peak at m/z 429, consistent with the molecular formula C₃₀H₅₂O [1] . - Conclusion: The study concludes that epifriedelanol demonstrates significant in vitro antitumor properties in the potato disc bioassay, warranting further investigation. However, it also notes a previous report where it showed no cytotoxicity against five human tumor cell lines at 10 μg/mL, suggesting its antitumor effect in the potato disc assay may not directly translate to general cytotoxicity against human cancer cells [1] . |
| Molecular Formula |
C30H52O
|
|---|---|
| Molecular Weight |
428.74
|
| Exact Mass |
428.401
|
| CAS # |
16844-71-6
|
| PubChem CID |
119242
|
| Appearance |
White to off-white solid powder
|
| Density |
1.0±0.1 g/cm3
|
| Boiling Point |
478.3±13.0 °C at 760 mmHg
|
| Flash Point |
199.6±12.3 °C
|
| Vapour Pressure |
0.0±2.7 mmHg at 25°C
|
| Index of Refraction |
1.509
|
| LogP |
11.45
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
31
|
| Complexity |
741
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
C[C@H]1[C@H](CC[C@@H]2[C@@]1(CC[C@H]3[C@]2(CC[C@@]4([C@@]3(CC[C@@]5([C@H]4CC(CC5)(C)C)C)C)C)C)C)O
|
| InChi Key |
XCDQFROEGGNAER-PFOIMGGJSA-N
|
| InChi Code |
InChI=1S/C30H52O/c1-20-21(31)9-10-22-27(20,5)12-11-23-28(22,6)16-18-30(8)24-19-25(2,3)13-14-26(24,4)15-17-29(23,30)7/h20-24,31H,9-19H2,1-8H3/t20-,21-,22+,23-,24+,26+,27+,28-,29+,30-/m0/s1
|
| Chemical Name |
(3S,4R,4aS,6aS,6aS,6bR,8aR,12aR,14aS,14bS)-4,4a,6a,6b,8a,11,11,14a-octamethyl-1,2,3,4,5,6,6a,7,8,9,10,12,12a,13,14,14b-hexadecahydropicen-3-ol
|
| Synonyms |
Epifriedelanol Friedelan-3beta-ol Longan triterpane A
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : < 0.1 mg/mL
DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3324 mL | 11.6621 mL | 23.3242 mL | |
| 5 mM | 0.4665 mL | 2.3324 mL | 4.6648 mL | |
| 10 mM | 0.2332 mL | 1.1662 mL | 2.3324 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
