| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 100mg |
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| Other Sizes |
| Targets |
D-Ribose 1,5-diphosphate is a substrate for the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), the most abundant enzyme on Earth. In the Calvin cycle, RuBisCO catalyzes the carboxylation of RuBP by CO2 to produce two molecules of 3-phosphoglycerate. This compound is also a key intermediate in the pentose phosphate pathway in some organisms.
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| ln Vitro |
This compound is not a drug and does not exert pharmacological activity in animals. Its primary function is as a substrate for the enzyme RuBisCO. It is widely used as a substrate for in vitro RuBisCO assays to study the kinetics and mechanisms of this pivotal enzyme. Its role is purely biochemical, forming the foundation of carbon fixation.
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| ln Vivo |
D-Ribose 1,5-diphosphate is not used in animal models for therapeutic purposes. Its role in the animal kingdom is limited, as animals lack RuBisCO and do not perform photosynthesis. Its "in vivo activity" is strictly confined to the realm of plant physiology. It is used in research as a substrate to study plant metabolism or as a standard for analytical chemistry.
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| Enzyme Assay |
A typical RuBisCO assay is performed in a cuvette in a spectrophotometer. Purified RuBisCO enzyme is added to a reaction buffer containing D-Ribose 1,5-diphosphate (RuBP) and NaH14CO3 or NaHCO3. The reaction is initiated by adding the RuBP. After a short incubation (e.g., 5 minutes) at 25degC, the reaction is stopped by adding acid. The non-reacted RuBP is removed, and the amount of acid-stable 14C-labeled 3-phosphoglycerate is measured by liquid scintillation counting. This assay is used to determine the enzyme's activity and inhibition.
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| Cell Assay |
As a substrate, it is not typically used for standard cell viability assays in animal cell cultures. Its cellular role is studied in the context of plant or cyanobacterial systems. For instance, in isolated plant chloroplasts or cyanobacterial lysates, the production of 3-phosphoglycerate from D-Ribose 1,5-diphosphate can be measured to study the efficiency of the Calvin cycle under various genetic or environmental perturbations.
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| Animal Protocol |
In vivo animal experiments are not applicable for this compound. If used in an animal, it would be part of a basic metabolic tracer study (e.g., using 14C-labeled compound) to examine its absorption and excretion, but it is not biologically relevant to animal physiology. A protocol might involve injecting the labeled compound into a mouse and tracking its conversion to CO2 or its incorporation into other metabolites via mass spectrometry.
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| ADME/Pharmacokinetics |
The compound is a small, highly polar molecule that is not orally bioavailable. It is chemically stable in aqueous solution. As a charged phosphate ester, it does not readily cross cell membranes. Pharmacokinetics are not studied for this compound, as it is not a drug candidate. Its key physical properties for research include high water solubility and stability under neutral pH conditions.
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| Toxicity/Toxicokinetics |
D-Ribose 1,5-diphosphate is an endogenous metabolite and is considered non-toxic at typical research concentrations. As a phosphorylated sugar, high systemic doses are not expected to be toxic, as animals do not possess the primary enzyme (RuBisCO) that uses it. It would be handled and disposed of using standard laboratory safety practices for general chemicals.
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| Additional Infomation |
D-ribulose-1,5-bisphosphate is a ribulose phosphate composed of D-ribulose with phosphate groups linked at positions 1 and 5. It is an intermediate in photosynthesis, a metabolite of both E. coli and plants. Functionally, it is related to D-ribulose and is the conjugate acid of D-ribulose-1,5-bisphosphate (4-). D-ribulose-1,5-bisphosphate is present in or produced by E. coli (K12 strain, MG1655 strain). D-ribulose-1,5-bisphosphate has also been reported to exist in rice, with relevant data available.
Toxicological data is not routinely generated for this biochemical reagent. |
| Molecular Formula |
C5H12O11P2
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|---|---|
| Molecular Weight |
310.08978
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| Exact Mass |
309.985
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| CAS # |
14689-84-0
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| PubChem CID |
123658
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| Appearance |
White to off-white solid powder
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| Density |
2.0±0.1 g/cm3
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| Boiling Point |
751.6±70.0 °C at 760 mmHg
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| Flash Point |
408.4±35.7 °C
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| Vapour Pressure |
0.0±5.7 mmHg at 25°C
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| Index of Refraction |
1.580
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| LogP |
-2.54
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
18
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| Complexity |
368
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| Defined Atom Stereocenter Count |
2
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| SMILES |
OP(OC[C@H]1OC(OP(=O)(O)O)[C@H](O)[C@@H]1O)(O)=O
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| InChi Key |
YAHZABJORDUQGO-NQXXGFSBSA-N
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| InChi Code |
InChI=1S/C5H12O11P2/c6-3(1-15-17(9,10)11)5(8)4(7)2-16-18(12,13)14/h3,5-6,8H,1-2H2,(H2,9,10,11)(H2,12,13,14)/t3-,5-/m1/s1
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| Chemical Name |
[(2R,3R)-2,3-dihydroxy-4-oxo-5-phosphonooxypentyl] dihydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2249 mL | 16.1244 mL | 32.2487 mL | |
| 5 mM | 0.6450 mL | 3.2249 mL | 6.4497 mL | |
| 10 mM | 0.3225 mL | 1.6124 mL | 3.2249 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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