| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Isotope-labeled phosphatidylcholine; no specific receptor or enzyme target. DPPC is a zwitterionic phosphoglyceride used for liposomal monolayers and lung surfactant preparations; the d71 form serves as an analytical internal standard and biophysical tracer without altering the lipid's membrane properties.
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|---|---|
| ln Vitro |
Drug compounds have been created with stable heavy isotopes of hydrogen, carbon, and other elements, mostly as quantitative tracers throughout the drug development process. Drug pharmacokinetics and metabolic properties may be affected by deuteration, which raises concerns [3].
The +71 Da mass shift places the d71 signal in a spectral region with minimal background interference; the compound is not used for potency screening but serves as an internal standard for DPPC quantification by LC-MS/MS and as a probe for membrane dynamics by ²H NMR. |
| ln Vivo |
No intrinsic in vivo pharmacological activity has been reported; DPPC-d71 is used as an isotope-dilution internal standard in liposomal formulation and biodistribution studies, and as a biophysical tracer for membrane organization and lipid–protein interactions.
|
| Enzyme Assay |
LC-MS/MS DPPC quantification: add DPPC-d71 to the sample before lipid extraction, extract phospholipids, separate by reversed-phase HPLC, and quantify DPPC by the analyte-to-d71 peak-area ratio using the d71-specific m/z transition, which is well separated from endogenous DPPC and other phospholipids.
|
| Cell Assay |
Cell-based membrane-labeling and lipid-mixing assay: incubate cells with DPPC-d71-containing liposomes, wash, lyse, extract lipids, and quantify the d71 lipid by LC-MS/MS; the large mass shift enables sensitive detection of liposome–cell membrane lipid exchange.
|
| Animal Protocol |
In vivo biodistribution and membrane-tracer study: administer DPPC-d71-labeled liposomes, collect plasma and tissues, extract lipids, and quantify the d71 signal by LC-MS/MS; the data are used to profile liposome pharmacokinetics and to trace DPPC incorporation into tissue membranes.
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| References |
|
| CAS # |
474943-49-2
|
|---|---|
| Related CAS # |
DPPC;63-89-8
|
| Appearance |
White to off-white solid
|
| SMILES |
[2H]C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C(O[C@H](COC(C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])[2H])=O)COP(OCC[N+](C([2H])([2H])[2H])(C([2H])([2H])[2H])C([2H])([2H])[2H])([O-])=O)=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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