| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
DNP-X, SE targets primary amine groups in proteins, peptides, and other biomolecules. It reacts with amines to form stable amide bonds, allowing for the covalent attachment of the dinitrophenyl (DNP) moiety to target molecules. The DNP moiety can then be recognized by anti-DNP antibodies. As a FRET quencher, it can be paired with Trp or Tyr to quench their fluorescence.
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| ln Vitro |
DNP-X, SE is not a therapeutic agent with pharmacological in vitro activity. It is a biochemical reagent used for labeling and detection in research applications. It is used to develop probes recognized by anti-DNP antibodies and as a FRET quencher.
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| ln Vivo |
DNP-X, SE is not used as a therapeutic agent in vivo. It is a biochemical reagent used in research applications. No in vivo pharmacological activity data is relevant.
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| Enzyme Assay |
DNP-X, SE is not used in enzyme/receptor binding assays as a test compound. It is used as a labeling reagent. The compound reacts with primary amines to form stable amide bonds. This reaction can be used to label proteins or peptides with the DNP moiety for detection by anti-DNP antibodies or for FRET-based assays.
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| Cell Assay |
DNP-X, SE is not used in cell-based pharmacological assays as a test compound. It is used as a labeling reagent for proteins or peptides that are then used in cellular studies.
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| Animal Protocol |
Not applicable.
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| ADME/Pharmacokinetics |
(DNP-X, SE (CAS 82321-04-8) has a molecular formula of C16H18N4O8 and a molecular weight of 394.34 g/mol. Chemical name: 2,5-dioxopyrrolidin-1-yl 6-((2,4-dinitrophenyl)amino)hexanoate. Appearance: typically a solid. Solubility: DMSO and other organic solvents. Storage: typical for NHS esters (desiccated, protected from light, -20°C). Purity: 98%.
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| Toxicity/Toxicokinetics |
No toxicity data is publicly available. As a laboratory reagent, DNP-X, SE should be handled with standard laboratory safety precautions. It is not intended for human consumption or therapeutic use.
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| Additional Infomation |
DNP-X, SE is a research-grade biochemical reagent, not a drug. It is used for developing probes recognized by anti-DNP antibodies and as an amine-reactive FRET quencher paired with Trp or Tyr. No clinical trials have been reported.
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| Molecular Formula |
C16H18N4O8
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|---|---|
| Molecular Weight |
394.33600
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| Exact Mass |
394.112
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| CAS # |
82321-04-8
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| PubChem CID |
16217139
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
591.8±60.0 °C at 760 mmHg
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| Melting Point |
152ºC
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| Flash Point |
311.7±32.9 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.606
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| LogP |
1.6
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
28
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| Complexity |
613
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IYBNUJCDIAGXNX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H18N4O8/c21-14-7-8-15(22)18(14)28-16(23)4-2-1-3-9-17-12-6-5-11(19(24)25)10-13(12)20(26)27/h5-6,10,17H,1-4,7-9H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 6-(2,4-dinitroanilino)hexanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~126.79 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5359 mL | 12.6794 mL | 25.3588 mL | |
| 5 mM | 0.5072 mL | 2.5359 mL | 5.0718 mL | |
| 10 mM | 0.2536 mL | 1.2679 mL | 2.5359 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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