| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
DNP-X acid does not target a specific biological receptor but functions as a chemical label or quencher. Its primary applications involve conjugation to biomolecules such as peptides, proteins, and antibodies. The dinitrophenyl (DNP) group serves as a hapten that can be recognized by anti-DNP antibodies, enabling detection in immunoassays. As a FRET quencher, it absorbs energy from fluorescent donors (Trp or Tyr) and dissipates it non-radiatively.
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| ln Vitro |
In vitro, DNP-X acid is used as an amine-reactive building block for developing probes that can be recognized by anti-DNP antibodies. It is also an excellent FRET quencher paired with tryptophan or tyrosine. The compound is soluble in DMSO (45 mg/mL) and has a melting point of 132.0-136.0°C. Its amine-reactive properties enable covalent attachment to primary amines on biomolecules.
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| ln Vivo |
DNP-X acid is not typically used in vivo as a therapeutic agent. However, DNP-labeled probes developed using this compound can be used in vivo for imaging or detection purposes, provided the probes are appropriately formulated. The DNP moiety is well-tolerated in vivo and has been used in various immunological and imaging studies.
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| Enzyme Assay |
For in vitro conjugation protocols, DNP-X acid is typically coupled to amine-containing biomolecules (e.g., proteins, peptides) using carbodiimide chemistry. The compound is dissolved in DMSO or DMF and mixed with the biomolecule in a suitable buffer (pH 7-8). A coupling agent such as EDC or HATU is added to activate the carboxylic acid group. The reaction is incubated for 1-4 hours at room temperature, and the conjugated product is purified by dialysis or chromatography.
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| Cell Assay |
For in vitro cell-based assays, DNP-X acid-conjugated probes can be used to label cells or cellular components. Cells are incubated with the DNP-labeled probe, and binding is detected using anti-DNP antibodies coupled to fluorescent or enzymatic reporters. The compound's FRET quenching properties can also be utilized in assays to monitor biomolecular interactions or enzyme activities in cell lysates or live cells.
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| Animal Protocol |
For in vivo animal experiments, DNP-X acid-conjugated probes can be administered to animals for imaging or biodistribution studies. The probes are typically injected intravenously or intraperitoneally. Blood and tissue samples are collected at various time points, and DNP-labeled materials are detected using anti-DNP antibodies or by measuring the fluorescence of the probe. The compound itself is not administered directly in vivo.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for DNP-X acid are limited. As a small molecule with a molecular weight of 297.26 g/mol and formula C₁₂H₁₅N₃O₆, it is expected to be rapidly cleared from circulation. When conjugated to larger biomolecules, the pharmacokinetics are determined by the carrier molecule. The compound is not intended for systemic therapeutic use.
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| Toxicity/Toxicokinetics |
Toxicological data for DNP-X acid are limited. As a dinitrophenyl compound, it may cause skin sensitization or allergic reactions in susceptible individuals. It is classified as a research-grade reagent and is not for human therapeutic use. Standard laboratory safety practices should be followed when handling the compound.
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| Additional Infomation |
N-(2,4-dinitrophenyl)aminohexanoic acid is a C-nitro compound consisting of hexanoic acid with a (2,4-dinitrophenyl)amino substituent attached at the C-6 position. Functionally, it is related to 6-aminohexanoic acid.
DNP-X acid is a research-use only compound and has not been approved for clinical applications. It is also known as N-(2,4-dinitrophenyl)-6-aminohexanoic acid. Its molecular weight is 297.26, and its formula is C₁₂H₁₅N₃O₆. The compound is available from various research chemical suppliers for probe development and FRET-based assay applications. |
| Molecular Formula |
C12H15N3O6
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|---|---|
| Molecular Weight |
297.2640
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| Exact Mass |
297.096
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| CAS # |
10466-72-5
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| PubChem CID |
96812
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| Appearance |
Light yellow to yellow solid powder
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| Melting Point |
133 °C
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| Index of Refraction |
1.626
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| LogP |
3.679
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
21
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| Complexity |
378
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZYUWUKIAUDIXCQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H15N3O6/c16-12(17)4-2-1-3-7-13-10-6-5-9(14(18)19)8-11(10)15(20)21/h5-6,8,13H,1-4,7H2,(H,16,17)
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| Chemical Name |
6-(2,4-dinitroanilino)hexanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 20.83 mg/mL (~70.07 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3641 mL | 16.8203 mL | 33.6406 mL | |
| 5 mM | 0.6728 mL | 3.3641 mL | 6.7281 mL | |
| 10 mM | 0.3364 mL | 1.6820 mL | 3.3641 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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