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| Other Sizes |
| Targets |
Dimethylglyoxime does not have a specific biological target. It acts as a chelating agent that forms stable complexes with metal ions, particularly nickel. The compound binds to metals such as iron and copper found in wastewater and industrial effluents. It can also inhibit or slow down the aggregation of Aβ peptide in vitro. Its mechanism is based on metal chelation rather than receptor or enzyme inhibition.
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| ln Vitro |
In vitro, dimethylglyoxime acts as a specific chelating agent for nickel. It can inhibit or slow down the aggregation of Aβ peptide. The compound's chelating activity is assessed by measuring the formation of stable complexes with metal ions such as Ni²⁺, Fe³⁺, and Cu²⁺. It is used as an antimicrobial agent and as an analytical reagent for metal ion detection.
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| ln Vivo |
In vivo, dimethylglyoxime is not used as a therapeutic agent. It is primarily used as an analytical reagent and chelating agent in laboratory and industrial applications. The compound binds to metals in wastewater and industrial effluents. It is not administered to animals or humans for pharmacological purposes. Any biological activity would be incidental and not the primary purpose of the compound.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not typically performed for dimethylglyoxime, as it is not a bioactive compound. The compound is used as a chelating agent and analytical reagent. Its purity and chemical properties are characterized by standard analytical techniques including NMR, HPLC, and mass spectrometry rather than by biological assays.
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| Cell Assay |
In vitro cell-based assays are not applicable for dimethylglyoxime as it is not a pharmacologically active compound. The compound is used in chemical analysis and as a chelating agent. It is not evaluated for its effects on cell viability, proliferation, or other cellular functions. It is not intended for use in cell culture or biological research applications.
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| Animal Protocol |
In vivo animal studies are not performed for dimethylglyoxime as it is not a pharmacologically active compound. The compound is used as a chemical reagent and analytical standard. Any animal studies involving this compound would be limited to toxicological evaluations for occupational safety or environmental impact rather than pharmacological efficacy studies.
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| ADME/Pharmacokinetics |
Dimethylglyoxime has a molecular formula of C4H8N2O2 and a molecular weight of 116.12. It has a melting point of 240-241 °C. The compound is soluble in alcohol, acetone, and ether. It is stored at 2-8°C or at room temperature in a cool and dark place. The compound is a white to off-white powder.
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| Toxicity/Toxicokinetics |
Toxicity data for dimethylglyoxime are limited to standard safety data sheets for handling and occupational exposure. The compound may cause skin and eye irritation upon contact. Appropriate safety precautions should be taken during handling. It is not classified as a hazardous substance at typical use concentrations. The compound should be stored and used in accordance with standard laboratory safety practices.
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| Additional Infomation |
Dimethylglyoxime is a well-known reagent in analytical chemistry. It is widely used as an analytical reagent for the quantitative analysis of nickel, iron, copper, and other heavy metal ions. The compound forms stable complexes with these metal ions and has excellent chromatographic properties. It is also used as a chelating agent and antimicrobial agent. Dimethylglyoxime is used primarily for research and analytical purposes.
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| Molecular Formula |
C4H8N2O2
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|---|---|
| Molecular Weight |
116.12
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| Exact Mass |
116.059
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| CAS # |
95-45-4
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| PubChem CID |
135399895
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| Appearance |
White to off-white solid powder
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| Density |
0.8
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| Boiling Point |
271.529ºC at 760 mmHg
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| Melting Point |
240-241 °C(lit.)
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| Flash Point |
14ºC
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| Index of Refraction |
1.487
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| LogP |
0.686
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
8
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| Complexity |
112
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| Defined Atom Stereocenter Count |
0
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| SMILES |
ON=C(C)C(C)=NO
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| InChi Key |
JGUQDUKBUKFFRO-GGWOSOGESA-N
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| InChi Code |
InChI=1S/C4H8N2O2/c1-3(5-7)4(2)6-8/h7-8H,1-2H3/b5-3+,6-4+
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| Chemical Name |
(NE)-N-[(3E)-3-hydroxyiminobutan-2-ylidene]hydroxylamine
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| Synonyms |
AI3 14925; AI3-14925; Dimethylglyoxime
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.6118 mL | 43.0589 mL | 86.1178 mL | |
| 5 mM | 1.7224 mL | 8.6118 mL | 17.2236 mL | |
| 10 mM | 0.8612 mL | 4.3059 mL | 8.6118 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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