| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
Geranylgeranylation is hampered by digeranyl bisphosphonate (DGBP). Cells were treated to either vehicle or digeranyl diphosphonate in order to investigate if the latter affects Rac1 activity. Chrysotile exposure markedly enhanced Rac1 activation, while geranylbisphosphonate-treated cells showed a return to control levels of activity. Moreover, digeranyl bisphosphonate decreases the generation of H2O2 in macrophages exposed to chrysotile [1].
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| ln Vivo |
To further examine the role of digeranyl bisphosphonate (DGBP; 0.2 mg/kg/day) in protecting mice from chrysotile-induced lung fibrosis, animals were subcutaneously injected with vehicle or digeranyl bisphosphonate in an osmotic pump. geranyl bisphosphonate and being exposed to chlorotile or saline the following day. Mice exposed to saline exhibited normal lung anatomy after treatment with vehicle and digeranyl diphosphonate. Chrysotile-exposed animals that received vehicle displayed severe structural abnormalities in the lung parenchyma and had extensive collagen deposition, whereas the lungs of geranylbisphosphonate-treated mice were largely normal. To study the role of digeranyl diphosphonate in bleomycin-induced fibrosis, osmotic pumps containing vehicle or digeranyl diphosphonate were implanted subcutaneously in WT mice. Mice were exposed to saline or bleomycin the next day. Mice treated with diceranyl bisphosphonate (0.2 mg/kg/day) displayed significantly less hydroxyproline compared to vehicle-treated mice exposed to bleomycin [1].
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| References |
| Molecular Formula |
C21H38NAO6P2
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|---|---|
| Molecular Weight |
471.460119724274
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| Exact Mass |
536.142
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| CAS # |
878143-03-4
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| PubChem CID |
11606262
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
33
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| Complexity |
654
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(P(O)(O)=O)(P(O)(O)=O)(C/C=C(\C)/CC/C=C(\C)/C)C/C=C(\C)/CC/C=C(\C)/C.[Na]
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| InChi Key |
MPGGMSSLQRFXRP-DRLFUFNGSA-J
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| InChi Code |
InChI=1S/C21H38O6P2.4Na/c1-17(2)9-7-11-19(5)13-15-21(28(22,23)24,29(25,26)27)16-14-20(6)12-8-10-18(3)4;;;;/h9-10,13-14H,7-8,11-12,15-16H2,1-6H3,(H2,22,23,24)(H2,25,26,27);;;;/q;4*+1/p-4/b19-13+,20-14+;;;;
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| Chemical Name |
tetrasodium;dioxido-oxo-[(6E,11E)-2,6,12,16-tetramethyl-9-phosphonatoheptadeca-2,6,11,15-tetraen-9-yl]-λ5-phosphane
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~2 mg/mL (~3.73 mM)
H2O : ~2 mg/mL (~3.73 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1211 mL | 10.6054 mL | 21.2107 mL | |
| 5 mM | 0.4242 mL | 2.1211 mL | 4.2421 mL | |
| 10 mM | 0.2121 mL | 1.0605 mL | 2.1211 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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