| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Di-4-ANEPPDHQ targets cell membrane lipid environments, specifically distinguishing between liquid-ordered (Lo) and liquid-disordered (Ld) phases. It assesses lipid accumulation via fluorescence spectrum shifts.
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|---|---|
| ln Vitro |
The dye exhibits a fluorescence spectrum shift upon embedding in membrane environments. In lipid phases, Lo phase yields emission at 560 nm, while Ld phase yields emission at 650 nm when excited at 488 nm. Low aqueous fluorescence and high membrane fluorescence are maintained.
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| ln Vivo |
No in vivo activity data are documented as Di-4-ANEPPDHQ is typically used for ex vivo live-cell imaging of dissociated cells or tissues, not for systemic in vivo administration.
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| Enzyme Assay |
This information is not applicable as Di-4-ANEPPDHQ is a membrane probe that interacts with lipid environments, not with purified enzymes or receptors in cell-free systems. For binding studies, liposomes are prepared, and fluorescence spectra are monitored upon dye incorporation to assess phase partitioning.
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| Cell Assay |
Live cells are loaded with 1-10 uM Di-4-ANEPPDHQ in culture medium for 15-30 min at 37degC. After washing, cells are imaged using confocal or two-photon microscopy with excitation at 488 nm and emission collected at 560 nm and 650 nm to monitor membrane order.
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| Animal Protocol |
Di-4-ANEPPDHQ is for ex vivo imaging only, not for in vivo animal administration. When imaging tissues, animals are euthanized, and dissected tissues are incubated with 5 uM dye for 30 min at room temperature prior to imaging.
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| ADME/Pharmacokinetics |
The dye is stable as a powder at -20degC for 3 years and as a solution at -80degC for 1 year. For in vitro use, DMSO is recommended: 80 mg/mL (120.2 mM). Ex/Em maxima: 488/560 nm (Lo phase) and 488/650 nm (Ld phase).
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| Toxicity/Toxicokinetics |
Standard laboratory safety precautions apply. Users should avoid inhalation and skin contact, and use personal protective equipment. The dye should be handled in a well-ventilated area with minimal light exposure.
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| References | |
| Additional Infomation |
This compound is a research-use-only fluorescent probe, not an approved pharmaceutical. It is highly sensitive to polarity changes, allowing its use in studies of membrane tension, lipid raft dynamics, and transmembrane potential. Molecular formula: C32H47Br2N3O2; molecular weight: 665.54.
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| Molecular Formula |
C32H47BRN3O2
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|---|---|
| Molecular Weight |
585.638488054276
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| Exact Mass |
665.201
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| CAS # |
797785-10-5
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| PubChem CID |
52920195
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| Appearance |
Brown to reddish brown solid powder
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
39
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| Complexity |
638
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
N(C1C=CC2C=C(C=CC3=CC=[N+](CC(O)C[N+](C)(C)CCO)C=C3)C=CC=2C=1)(CCCC)CCCC.[Br-]
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| InChi Key |
CRHKZEFQMHRRPS-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C32H47N3O2.2BrH/c1-5-7-17-34(18-8-6-2)31-14-13-29-23-28(11-12-30(29)24-31)10-9-27-15-19-33(20-16-27)25-32(37)26-35(3,4)21-22-36;;/h9-16,19-20,23-24,32,36-37H,5-8,17-18,21-22,25-26H2,1-4H3;2*1H/q+2;;/p-2
|
| Chemical Name |
[3-[4-[(E)-2-[6-(dibutylamino)naphthalen-2-yl]ethenyl]pyridin-1-ium-1-yl]-2-hydroxypropyl]-(2-hydroxyethyl)-dimethylazanium;dibromide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7075 mL | 8.5377 mL | 17.0753 mL | |
| 5 mM | 0.3415 mL | 1.7075 mL | 3.4151 mL | |
| 10 mM | 0.1708 mL | 0.8538 mL | 1.7075 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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