| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Target: pH-sensitive fluorescent probe / Intracellular pH indicator. DHPDS disodium salt functions as a fluorescent dye that is sensitive to changes in pH, allowing real-time monitoring of intracellular pH in living cells and tissues. It accumulates in cells and its fluorescence intensity varies with pH, enabling quantitative pH measurements.
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| ln Vitro |
In vitro, DHPDS disodium salt is used as a pH-sensitive fluorescent probe in cell-based assays to measure intracellular pH changes. It is loaded into cells, and upon excitation at appropriate wavelengths, its fluorescence emission intensity varies proportionally to the pH of the cellular environment. No direct pharmacological activity is reported.
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| ln Vivo |
In vivo, DHPDS disodium salt has been used to measure intracellular pH (pHi) from the surface fluorescence of isolated perfused rat liver. It is not used for therapeutic purposes but rather as a research tool to study pH regulation in organ perfusion models and to assess cellular metabolic states under various conditions.
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| Enzyme Assay |
For cell-free assays: DHPDS disodium salt is dissolved in an appropriate buffer solution at a concentration of 10-100 uM. Excitation is performed at 350-400 nm, and emission is measured at 450-550 nm. Fluorescence intensity is recorded using a spectrofluorometer or plate reader, and pH calibration curves are constructed using standard buffers of known pH.
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| Cell Assay |
For cell assays: cells are loaded with DHPDS disodium salt (typically 5-50 uM) in culture medium for 30-60 min at 37degC, washed to remove extracellular dye, and then imaged using a fluorescence microscope or plate reader. The ratio of fluorescence at two excitation wavelengths (e.g., 350/400 nm) is used to calculate intracellular pH.
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| Animal Protocol |
For animal studies: in the isolated perfused rat liver model, the liver is perfused with buffer containing DHPDS disodium salt. Surface fluorescence is measured using a fiber-optic probe or imaging system. The fluorescence ratio is calibrated to determine intracellular pH (pHi) in real time, enabling studies of hepatic pH regulation and metabolic function.
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| ADME/Pharmacokinetics |
PK properties are not reported for DHPDS disodium salt as it is a fluorescent dye used for ex vivo and in vitro applications, not a systemically administered drug. It is typically used for loading into cells or isolated organs, and its fluorescence decay and retention are dependent on the experimental conditions rather than systemic ADME parameters.
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| Toxicity/Toxicokinetics |
No significant toxicity is reported for DHPDS disodium salt at typical experimental concentrations (5-50 uM). It is for research use only and not intended for human therapeutic applications. As a fluorescent dye, it may cause mild phototoxicity under prolonged light exposure, but no acute systemic toxicity has been documented.
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| References | |
| Additional Infomation |
DHPDS disodium salt is a research reagent, not a drug. It is not FDA-approved for clinical use. It is widely used in cell biology and physiology research for monitoring intracellular pH dynamics, studying pH regulation in disease models (e.g., ischemia, cancer, inflammation), and evaluating drug effects on cellular pH homeostasis. It is also used as a fluorescent probe in high-content screening applications.
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| Molecular Formula |
C16H8O8S2-2.2[NA+]
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|---|---|
| Molecular Weight |
438.33952
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| Exact Mass |
437.946
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| CAS # |
61255-63-8
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| PubChem CID |
16212389
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| Appearance |
Light yellow to green yellow solid powder
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| Melting Point |
≥250ºC(lit.)
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| LogP |
3.965
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
28
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| Complexity |
678
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JISMNCQKTAIERN-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C16H10O8S2.2Na/c17-11-5-12(18)8-2-4-10-14(26(22,23)24)6-13(25(19,20)21)9-3-1-7(11)15(8)16(9)10;;/h1-6,17-18H,(H,19,20,21)(H,22,23,24);;/q;2*+1/p-2
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| Chemical Name |
disodium;6,8-dihydroxypyrene-1,3-disulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2813 mL | 11.4067 mL | 22.8133 mL | |
| 5 mM | 0.4563 mL | 2.2813 mL | 4.5627 mL | |
| 10 mM | 0.2281 mL | 1.1407 mL | 2.2813 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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