| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The primary analytical target is desoxycarbadox as the marker residue of carbadox exposure. Carbadox itself acts as an antibacterial and growth promoter through mechanisms that are not fully defined but may involve DNA damage and effects on the gut microbiome; desoxycarbadox is the metabolite used for residue monitoring. The d3-labeled form serves as an isotope-dilution internal standard for LC-MS/MS quantification.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Desoxycarbadox-d3 is used as an internal standard for LC-MS/MS quantification of desoxycarbadox in swine tissues. The labeled standard corrects for extraction recovery, ionization efficiency, and matrix effects, enabling accurate determination of carbadox residues at regulatory limits. The unlabeled metabolite has no established intrinsic pharmacological potency relevant to its use as a marker residue. |
| ln Vivo |
In vivo, carbadox is rapidly metabolized in swine to desoxycarbadox, which persists in liver and muscle and is used as the target analyte for residue monitoring and withdrawal-period determination. The d3-labeled standard supports in vivo residue-depletion studies by enabling precise quantification of desoxycarbadox in edible tissues.
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| Enzyme Assay |
LC-MS/MS residue analysis: add Desoxycarbadox-d3 to the sample before extraction; extract the metabolite with organic solvent (e.g., ethyl acetate or acetonitrile); separate by reversed-phase HPLC; monitor the specific m/z transition of the d3 standard and the corresponding unlabeled desoxycarbadox transition. Quantification is by isotope-dilution calibration using peak-area ratios.
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| Cell Assay |
In vitro metabolism assay: incubate carbadox with swine liver microsomes or hepatocytes, monitor desoxycarbadox formation over time, and quantify the metabolite by LC-MS/MS using the d3 standard to determine metabolic kinetics and to identify the enzymes responsible for N-oxide reduction.
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| Animal Protocol |
In vivo residue-depletion study: administer carbadox to swine at therapeutic doses, collect liver and muscle at defined withdrawal intervals, add Desoxycarbadox-d3 before extraction, and quantify desoxycarbadox by LC-MS/MS to establish withdrawal periods and monitor compliance with maximum residue limits.
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| References |
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| Molecular Formula |
C11H10N4O2
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|---|---|
| Molecular Weight |
233.241186618805
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| Exact Mass |
233.099
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| CAS # |
1448350-02-4
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| Related CAS # |
Desoxycarbadox;55456-55-8
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| PubChem CID |
9568819
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Index of Refraction |
1.634
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| LogP |
1.72
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
17
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| Complexity |
295
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])OC(=O)N/N=C/C1=NC2=CC=CC=C2N=C1
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| InChi Key |
SOEMFPLGOUBPJQ-QQAPZFQVSA-N
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| InChi Code |
InChI=1S/C11H10N4O2/c1-17-11(16)15-13-7-8-6-12-9-4-2-3-5-10(9)14-8/h2-7H,1H3,(H,15,16)/b13-7+/i1D3
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| Chemical Name |
trideuteriomethyl N-[(E)-quinoxalin-2-ylmethylideneamino]carbamate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2874 mL | 21.4371 mL | 42.8743 mL | |
| 5 mM | 0.8575 mL | 4.2874 mL | 8.5749 mL | |
| 10 mM | 0.4287 mL | 2.1437 mL | 4.2874 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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