| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
DEAC, SE targets amine groups on biomolecules, including proteins, peptides, and other amine-containing compounds. The N-hydroxysuccinimidyl (SE) ester moiety reacts with primary amines to form stable amide bonds, covalently attaching the coumarin fluorophore to the target molecule. The compound does not have a specific biological receptor target but is used as a fluorescent labeling tool. Its diethylaminocoumarin fluorophore provides blue fluorescence for detection and imaging applications.
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| ln Vitro |
DEAC, SE is a blue fluorescent dye that exhibits excellent fluorescence properties for labeling amine-containing biomolecules. The compound's N-hydroxysuccinimidyl ester reacts with primary amines to form stable amide bonds, enabling covalent attachment to proteins, peptides, and other biomolecules. The coumarin fluorophore provides blue fluorescence suitable for various detection and imaging applications. DEAC, SE has been used in life science research for labeling and tracking biomolecules.
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| ln Vivo |
In vivo activity data for DEAC, SE are not applicable, as the compound is primarily used as a fluorescent labeling reagent for in vitro applications rather than as a therapeutic agent. The compound may be used in vivo for imaging studies if conjugated to targeting molecules, but specific in vivo efficacy studies are not documented. DEAC, SE is typically used for labeling proteins, peptides, and other biomolecules for detection and analysis in research applications.
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| Enzyme Assay |
In vitro labeling assays for DEAC, SE typically involve incubating the compound with amine-containing biomolecules (such as proteins or peptides) in buffer at pH 7.5-8.5 for 30 minutes to several hours. The reaction forms stable amide bonds between the SE ester and primary amines. The labeled products are then analyzed by SDS-PAGE, HPLC, or mass spectrometry to confirm labeling efficiency. The compound is dissolved in anhydrous DMSO or DMF as a stock solution and diluted in reaction buffer.
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| Cell Assay |
In vitro cellular assays for DEAC, SE typically involve labeling cellular proteins or cell surface amines. Cells are incubated with the compound at concentrations typically ranging from 1 to 100 μM for 30 minutes to 2 hours, followed by washing to remove unbound dye. Labeled cells are then analyzed by flow cytometry or fluorescence microscopy. The compound can also be used to label purified proteins or peptides for various biochemical assays. The compound is dissolved in DMSO and diluted in cell culture medium or buffer.
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| Animal Protocol |
In vivo animal studies for DEAC, SE are not typically performed, as the compound is primarily used as a labeling reagent for in vitro applications. For potential in vivo imaging studies, the compound could be conjugated to targeting molecules and administered to animals via intravenous injection. Tissue samples could be collected and analyzed by fluorescence microscopy. However, specific protocols for in vivo studies with DEAC, SE are not well-established in the public domain.
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| ADME/Pharmacokinetics |
DEAC, SE has a molecular weight of 358.35 g/mol and formula C18H18N2O6. The compound is typically stored at -20°C as a powder. It is soluble in anhydrous DMSO or DMF and is typically prepared as a stock solution in these solvents for use in labeling reactions. The compound should be protected from moisture and light due to its reactivity with water and light sensitivity. Detailed PK parameters are not applicable for this labeling reagent.
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| Toxicity/Toxicokinetics |
Toxicity data for DEAC, SE are not extensively reported. The compound is intended for research use only and is not approved for human therapeutic applications. As with all chemical compounds, appropriate safety precautions should be taken when handling. The compound's reactivity with amines suggests potential for protein modification and possible toxicity at high concentrations. Standard toxicity assessments would include acute toxicity, genotoxicity, and repeated-dose toxicity studies.
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| Additional Infomation |
DEAC, SE (CAS 139346-57-9) is an excellent blue fluorescent building block for labeling amine-containing biomolecules. It has a molecular weight of 358.35 g/mol and formula C18H18N2O6. The compound is also known as 7-diethylaminocoumarin-3-carboxylic acid succinimidyl ester. DEAC, SE is used in life science research for labeling proteins, peptides, and other amine-containing biomolecules. The compound is not approved for clinical use and is intended for research applications.
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| Molecular Formula |
C18H18N2O6
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|---|---|
| Molecular Weight |
358.34532
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| Exact Mass |
358.116
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| CAS # |
139346-57-9
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| PubChem CID |
2760552
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.4g/cm3
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| Boiling Point |
559.3ºC at 760mmHg
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| Flash Point |
292.1ºC
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| Vapour Pressure |
1.53E-12mmHg at 25°C
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| Index of Refraction |
1.622
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| LogP |
1.797
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
26
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| Complexity |
638
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
NUNPVRICKDZFLK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 7-(diethylamino)-2-oxochromene-3-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~16.67 mg/mL (~46.52 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7906 mL | 13.9528 mL | 27.9057 mL | |
| 5 mM | 0.5581 mL | 2.7906 mL | 5.5811 mL | |
| 10 mM | 0.2791 mL | 1.3953 mL | 2.7906 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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