| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Cleavable Linker
Not applicable (linker/reagent). |
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| ln Vitro |
DBCO-acid is a click chemistry reagent used for bioconjugation. The DBCO moiety enables strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules. The carboxylic acid group allows further derivatization, such as the synthesis of NHS esters or other activated esters for conjugation to amines.
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| ln Vivo |
DBCO-acid is not a drug and has no direct in vivo activity. It is used as a chemical tool for the synthesis of bioconjugates, including antibody-drug conjugates (ADCs), for in vivo applications.
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| Enzyme Assay |
DBCO-acid is used in chemical reactions to demonstrate copper-free click chemistry. The reaction between DBCO-acid and azide-containing molecules is monitored by analytical techniques such as HPLC or mass spectrometry to confirm conjugation efficiency.
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| Cell Assay |
DBCO-acid is used in cell-based assays as a tool for labeling azide-modified biomolecules on cell surfaces or within cells. Cells are incubated with azide-containing probes, followed by DBCO-acid conjugates, and labeling is detected by fluorescence or other methods.
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| Animal Protocol |
DBCO-acid is used in animal models as part of ADC or bioconjugate formulations for targeted therapy. The DBCO-azide click chemistry enables efficient and site-specific conjugation of drugs or imaging agents to antibodies or other targeting moieties in vivo.
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| ADME/Pharmacokinetics |
No detailed PK data available. DBCO-acid has a molecular weight of 305.33. Soluble in DMSO (10 mM). Purity >98%. Storage at -20°C.
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| Toxicity/Toxicokinetics |
DBCO-acid has GHS hazard statements: H302 (harmful if swallowed), H315 (causes skin irritation), H319 (causes serious eye irritation), H335 (may cause respiratory irritation). For research use only.
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| References | |
| Additional Infomation |
DBCO-acid is a click chemistry linker. Molecular formula: C19H15NO3; MW: 305.33. Used in ADC linker synthesis. For research use only.
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| Molecular Formula |
C19H15NO3
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|---|---|
| Molecular Weight |
305.327305078506
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| Exact Mass |
305.105
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| Elemental Analysis |
C, 74.74; H, 4.95; N, 4.59; O, 15.72
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| CAS # |
1353016-70-2
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| Related CAS # |
DBCO-acid;1353016-70-2
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| PubChem CID |
91757805
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| Appearance |
Off-white to light brown solid powder
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| LogP |
2.862
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
23
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| Complexity |
529
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(CCC(=O)O)N1C2C=CC=CC=2C#CC2C=CC=CC=2C1
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| InChi Key |
NDLOVDOICXITOK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H15NO3/c21-18(11-12-19(22)23)20-13-16-7-2-1-5-14(16)9-10-15-6-3-4-8-17(15)20/h1-8H,11-13H2,(H,22,23)
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| Chemical Name |
11,12-Didehydro-γ-oxodibenz[b,f]azocine-5(6H)-butanoic acid
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| Synonyms |
DBCO-acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~327.51 mM )
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2751 mL | 16.3757 mL | 32.7514 mL | |
| 5 mM | 0.6550 mL | 3.2751 mL | 6.5503 mL | |
| 10 mM | 0.3275 mL | 1.6376 mL | 3.2751 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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