| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Cleavable Linker
Not applicable (linker/reagent). |
|---|---|
| ln Vitro |
DBCO-acid is a click chemistry reagent used for bioconjugation. The DBCO moiety enables strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules. The carboxylic acid group allows further derivatization, such as the synthesis of NHS esters or other activated esters for conjugation to amines.
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| ln Vivo |
DBCO-acid is not a drug and has no direct in vivo activity. It is used as a chemical tool for the synthesis of bioconjugates, including antibody-drug conjugates (ADCs), for in vivo applications.
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| Enzyme Assay |
DBCO-acid is used in chemical reactions to demonstrate copper-free click chemistry. The reaction between DBCO-acid and azide-containing molecules is monitored by analytical techniques such as HPLC or mass spectrometry to confirm conjugation efficiency.
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| Cell Assay |
DBCO-acid is used in cell-based assays as a tool for labeling azide-modified biomolecules on cell surfaces or within cells. Cells are incubated with azide-containing probes, followed by DBCO-acid conjugates, and labeling is detected by fluorescence or other methods.
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| Animal Protocol |
DBCO-acid is used in animal models as part of ADC or bioconjugate formulations for targeted therapy. The DBCO-azide click chemistry enables efficient and site-specific conjugation of drugs or imaging agents to antibodies or other targeting moieties in vivo.
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| ADME/Pharmacokinetics |
No detailed PK data available. DBCO-acid has a molecular weight of 305.33. Soluble in DMSO (10 mM). Purity >98%. Storage at -20°C.
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| Toxicity/Toxicokinetics |
DBCO-acid has GHS hazard statements: H302 (harmful if swallowed), H315 (causes skin irritation), H319 (causes serious eye irritation), H335 (may cause respiratory irritation). For research use only.
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| References | |
| Additional Infomation |
DBCO-acid is a click chemistry linker. Molecular formula: C19H15NO3; MW: 305.33. Used in ADC linker synthesis. For research use only.
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| Molecular Formula |
C19H15NO3
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|---|---|
| Molecular Weight |
305.327305078506
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| Exact Mass |
305.105
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| Elemental Analysis |
C, 74.74; H, 4.95; N, 4.59; O, 15.72
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| CAS # |
1353016-70-2
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| Related CAS # |
DBCO-acid;1353016-70-2
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| PubChem CID |
91757805
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| Appearance |
Off-white to light brown solid powder
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| LogP |
2.862
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
23
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| Complexity |
529
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(CCC(=O)O)N1C2C=CC=CC=2C#CC2C=CC=CC=2C1
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| InChi Key |
NDLOVDOICXITOK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H15NO3/c21-18(11-12-19(22)23)20-13-16-7-2-1-5-14(16)9-10-15-6-3-4-8-17(15)20/h1-8H,11-13H2,(H,22,23)
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| Chemical Name |
11,12-Didehydro-γ-oxodibenz[b,f]azocine-5(6H)-butanoic acid
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| Synonyms |
DBCO-acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~327.51 mM )
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.19 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.19 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (8.19 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2751 mL | 16.3757 mL | 32.7514 mL | |
| 5 mM | 0.6550 mL | 3.2751 mL | 6.5503 mL | |
| 10 mM | 0.3275 mL | 1.6376 mL | 3.2751 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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