| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
No pharmacological target. The compound is a stable isotope-labeled internal standard. Its analytical role is to co-elute with the unlabeled dapsone analyte and provide a distinct mass transition in LC-MS/MS, correcting for matrix effects, extraction recovery, and ionization variability in biological matrices.
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|---|---|
| ln Vitro |
In the process of developing new drugs, stable heavy isotopes of carbon, hydrogen, and other elements have been added to pharmacological molecules, mostly as quantitative tracers. Due to its potential effects on medication pharmacokinetics and metabolic properties, deuteration is a matter for worry [1].
No intrinsic biological activity is applicable. Relevant specifications include isotopic enrichment, chemical purity, and isotopic distribution. High 13C incorporation ensures that the +12 Da transition is free from interference by the natural isotope envelope of the unlabeled drug. |
| ln Vivo |
No in vivo therapeutic efficacy is expected. In vivo, the compound is used as an internal standard in pharmacokinetic and residue studies of dapsone.
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| Enzyme Assay |
In the analytical workflow, Dapsone-13C12 is spiked into plasma or tissue homogenates prior to extraction. LC-MS/MS analysis monitors the transition of unlabeled dapsone and the corresponding +12 Da transition of the internal standard. The peak area ratio is used for quantification against a calibration curve.
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| Cell Assay |
In cell-based systems, the compound serves as an internal standard for quantifying dapsone in cell culture media or lysates. Samples are spiked with the labeled standard before extraction and LC-MS/MS analysis.
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| Animal Protocol |
In animal experiments, Dapsone-13C12 is used as an internal standard in pharmacokinetic studies. Plasma or tissue samples are spiked with the labeled standard, extracted, and analyzed by LC-MS/MS, enabling accurate determination of dapsone concentrations.
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| References |
| Molecular Formula |
C12H12N2O2S
|
|---|---|
| Molecular Weight |
260.212738990784
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| Exact Mass |
248.061
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| CAS # |
1632119-29-9
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| Related CAS # |
Dapsone;80-08-0
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| PubChem CID |
117065483
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| Appearance |
White to off-white solid
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| LogP |
1
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
17
|
| Complexity |
306
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=S([13C]1=[13CH][13CH]=[13C]([13CH]=[13CH]1)N)([13C]2=[13CH][13CH]=[13C]([13CH]=[13CH]2)N)=O
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| InChi Key |
MQJKPEGWNLWLTK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H12N2O2S/c13-9-1-5-11(6-2-9)17(15,16)12-7-3-10(14)4-8-12/h1-8H,13-14H2
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| Chemical Name |
4-(4-aminophenyl)sulfonylaniline
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8430 mL | 19.2152 mL | 38.4305 mL | |
| 5 mM | 0.7686 mL | 3.8430 mL | 7.6861 mL | |
| 10 mM | 0.3843 mL | 1.9215 mL | 3.8430 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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