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| Other Sizes |
| Targets |
Dansyl chloride targets primary amino groups in aliphatic and aromatic amines. It reacts with primary amines to produce stable blue or blue-green fluorescent sulfonamide adducts. The compound is used for modified amino acid analysis, protein sequencing, and amino acid analysis. It can also be used to label hydroxyl and carboxylic acid functional groups. Dansyl chloride is a fluorogenic reagent that is non-fluorescent until it reacts with amines.
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| ln Vitro |
In vitro, Dansyl chloride is a fluorogenic reagent used for protein, N-terminal amino acid, and peptide detection by HPLC. It reacts with primary amines to produce stable blue or blue-green fluorescent sulfonamide adducts. The resulting dansyl amides have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. The compound is used to prepare fluorescent drug or ligand analogs that are expected to bind to hydrophobic sites in proteins or membranes.
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| ln Vivo |
In vivo, Dansyl chloride is used as a labeling reagent for research applications. Its utility in protein and peptide labeling has been established. The compound is used to prepare fluorescent drug or ligand analogs for binding studies. Further in vivo studies are limited as the compound is primarily a laboratory reagent.
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| Enzyme Assay |
In vitro derivatization assays for Dansyl chloride involve reacting the compound with primary amines under appropriate conditions. The reaction produces fluorescent sulfonamide adducts that are detected by HPLC with fluorescence detection. Derivatization efficiency is assessed by measuring fluorescence intensity. For amino acid analysis, amino acids are derivatized with Dansyl chloride and separated by HPLC. Assays are performed in appropriate buffer systems with positive controls such as known amine standards.
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| Cell Assay |
Dansyl chloride cell-based assays are limited as the compound is primarily a labeling reagent rather than a therapeutic compound. The compound is used to label cellular proteins or peptides for detection. Cells are treated with Dansyl chloride and fluorescence is measured. Cell viability is assessed by standard assays. Experiments are performed with appropriate positive and negative controls.
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| Animal Protocol |
Dansyl chloride in vivo studies are limited as the compound is primarily a laboratory reagent. Its utility in protein and peptide labeling has been established. The compound is used in research applications for amino acid analysis and protein sequencing. Studies are conducted in accordance with institutional guidelines for reagent use.
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| ADME/Pharmacokinetics |
Dansyl chloride (MW 269.74-269.75 g/mol, C12H12ClNO2S) appears as a light yellow to brown powder to crystal with a melting point of 68.0 to 73.0°C. Its purity is min. 98.0 area% by HPLC. The compound is stable under recommended storage conditions. Dansyl chloride is a fluorogenic reagent used for amino acid and peptide analysis.
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| Toxicity/Toxicokinetics |
Dansyl chloride is generally considered safe as a laboratory reagent. The compound is widely used in amino acid analysis and protein sequencing. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| References | |
| Additional Infomation |
Dansyl chloride is an aminonaphthalene and sulfonic acid derivative.
Dansyl chloride (5-dimethylaminonaphthalene-1-sulfonyl chloride) is a fluorogenic reagent that reacts with primary amines to produce stable blue-green fluorescent sulfonamide adducts. It is used for protein, amino acid, and peptide detection by HPLC. The compound is used for protein sequencing and amino acid analysis. Its molecular formula is C12H12ClNO2S with a molecular weight of 269.74-269.75 g/mol. All applications are limited to non-human research use. |
| Molecular Formula |
C12H12CLNO2S
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|---|---|
| Molecular Weight |
269.7472
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| Exact Mass |
269.027
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| CAS # |
605-65-2
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| Related CAS # |
Dansyl chloride-d6;1276379-68-0
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| PubChem CID |
11801
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
371.3±22.0 °C at 760 mmHg
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| Melting Point |
72-74 °C(lit.)
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| Flash Point |
178.4±22.3 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.636
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| LogP |
3.67
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
17
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| Complexity |
363
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
XPDXVDYUQZHFPV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
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| Chemical Name |
5-(dimethylamino)naphthalene-1-sulfonyl chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. (3). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~4.17 mg/mL (~15.46 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7071 mL | 18.5357 mL | 37.0714 mL | |
| 5 mM | 0.7414 mL | 3.7071 mL | 7.4143 mL | |
| 10 mM | 0.3707 mL | 1.8536 mL | 3.7071 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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