| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
As a color additive, D & C Orange no. 5 does not have a specific pharmacological target. It can be used as a ligand to study protein properties through spectroscopic analysis. The compound is a fluorescein analogue that absorbs and emits light at specific wavelengths, making it useful for fluorescence-based applications.
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| ln Vitro |
D & C Orange no. 5 is a fluorescein analogue that can be used as a ligand to study protein properties through spectroscopic analysis. It is a color additive used in lipsticks, mouthwashes, and dentifrices. The compound is a mixture of brominated fluorescein derivatives.
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| ln Vivo |
In vivo, D & C Orange no. 5 is used as a color additive in cosmetics and drugs. It is permitted for use in lipsticks, mouthwashes, and dentifrices. The compound is not intended for therapeutic use but as a coloring agent. Further in vivo studies are not applicable for this compound.
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| Enzyme Assay |
As a color additive, D & C Orange no. 5 does not have traditional enzyme/receptor binding assays. The compound's properties are characterized by its absorption and emission spectra. It can be used as a ligand to study protein properties through spectroscopic analysis.
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| Cell Assay |
In vitro assays for D & C Orange no. 5 involve measuring its spectroscopic properties, such as absorption and fluorescence spectra. The compound can be used as a fluorescent probe to study protein binding or other biological interactions. Its solubility and stability are also characterized.
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| Animal Protocol |
In vivo animal studies for D & C Orange no. 5 are not applicable as the compound is a color additive rather than a therapeutic agent. The compound's safety for use in cosmetics and drugs has been evaluated by the FDA.
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| ADME/Pharmacokinetics |
D & C Orange no. 5 has a molecular weight of 490.11 and a molecular formula of C20H10Br2O5. It is a fluorescein analogue that is soluble in hot 0.5 N NH4OH, producing a clear yellow to orange solution. The compound is a color additive permanently listed by the FDA.
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| Toxicity/Toxicokinetics |
Preclinical toxicity studies of D & C Orange no. 5 have been conducted to support its use as a color additive. The compound is permanently listed by the FDA for use in drugs and cosmetics, requiring certification. It is permitted for use in lipsticks, mouthwashes, and dentifrices.
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| References | |
| Additional Infomation |
D & C Orange no. 5 (4',5'-Dibromofluorescein, Eosinic acid) is a fluorescein analogue and color additive permitted for use in lipsticks, mouthwashes, and dentifrices. It is a mixture of brominated fluorescein derivatives and is permanently listed by the FDA, requiring certification for use in drugs and cosmetics. The compound can be used as a ligand to study protein properties through spectroscopic analysis.
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| Molecular Formula |
C20H8BR2NA2O5
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|---|---|
| Molecular Weight |
534.06
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| Exact Mass |
487.889
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| CAS # |
596-03-2
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| PubChem CID |
11689
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| Appearance |
Orange to red solid powder
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| Density |
2.1 g/cm3
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| Boiling Point |
633.7ºC at 760 mmHg
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| Melting Point |
270-273 °C(lit.)
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| Flash Point |
337ºC
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| Index of Refraction |
1.771
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| LogP |
5.19
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
27
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| Complexity |
585
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZDTNHRWWURISAA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
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| Chemical Name |
4',5'-dibromo-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
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| Synonyms |
CCRIS 4900; C.I. Solvent Red 72; D & C Orange no. 5
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~127.52 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8724 mL | 9.3622 mL | 18.7245 mL | |
| 5 mM | 0.3745 mL | 1.8724 mL | 3.7449 mL | |
| 10 mM | 0.1872 mL | 0.9362 mL | 1.8724 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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