| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
The primary target is Cholinesterase (ChE) within the Neuronal Signaling pathway. It is also involved in biochemical pathways related to phosphatidylethanolamine synthesis and lipid metabolism in cells.
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| ln Vitro |
No specific in vitro activity data beyond its role as a biochemical intermediate is available. As a stimulant of ACh synthesis, it may indirectly influence cholinergic neuronal activity in cell-based models. It is a soluble compound (≥50 mg/mL in H2O) suitable for various in vitro biochemical assays related to lipid metabolism and neurotransmitter synthesis.
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| ln Vivo |
No specific in vivo activity data is available. Its use in animal models would likely focus on studying phosphatidylethanolamine synthesis in tissues or the effects of enhanced ACh synthesis on neurological functions. The compound is typically prepared in saline or other aqueous solutions for administration.
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| Enzyme Assay |
In a cell-free system, this compound is utilized as a substrate to study enzymatic reactions involving phosphatidylethanolamine synthesis. The reaction mixture includes the compound (e.g., 1-10 mM), diacylglycerol, and the appropriate enzyme (e.g., CDP-ethanolamine transferase) in a buffered solution (pH 7.4). The production of phosphatidylethanolamine can be quantified via thin-layer chromatography (TLC) or mass spectrometry to measure enzyme activity.
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| Cell Assay |
For in vitro cellular assays, the compound is dissolved in sterile water or culture medium (at concentrations typically ranging from 10-100 microM) to treat neuronal or hepatocyte cell lines. After 24-48 hours of treatment, cellular phospholipid content is extracted and analyzed using HPLC or LC-MS. Acetylcholine levels in cell lysates can also be quantified using commercial ELISA kits to assess its stimulatory effect.
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| Animal Protocol |
For in vivo animal experiments, the compound is dissolved in saline or PBS. For pharmacokinetic studies, a single dose (e.g., 10-50 mg/kg) is administered to rodents via intravenous (IV) or intraperitoneal (IP) injection. Blood samples are collected at various time points (0-24h). The concentration of the compound and its metabolites in plasma and tissues is measured using validated LC-MS/MS methods to assess distribution and metabolism.
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| ADME/Pharmacokinetics |
As an endogenous biochemical intermediate, it possesses favorable solubility (≥50 mg/mL in H2O) and stability (powder stable for 3 years at -20degC). In vivo pharmacokinetics in rodents typically involves IV or IP administration, with plasma half-life and tissue distribution determined by LC-MS/MS following a single dose (e.g., 10 mg/kg).
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| Toxicity/Toxicokinetics |
For a naturally occurring biochemical intermediate, acute toxicity is expected to be low. No specific toxicological data is available for the isolated compound. Standard laboratory handling precautions apply. The compound should be stored as a powder at -20degC to maintain stability for up to 3 years.
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| References | |
| Additional Infomation |
CDP-ethanolamine is a phosphate-ethanolamine composed of an ethanolamine molecule and a cytidine 5'-bisphosphate group attached to an oxygen atom. It is a mouse metabolite. It belongs to the nucleotide-(amino alcohol) class and the phosphate-ethanolamine class. It is the conjugate acid of CDP-ethanolamine (1-). CDP-ethanolamine has been reported to exist in Daphnia pulex, Arabidopsis thaliana, and several other organisms with relevant data. CDP-ethanolamine is a metabolite found or produced in Saccharomyces cerevisiae.
This compound is a fundamental research tool in lipid biochemistry and neurochemistry. Also known as CDP-ethanolamine, it is crucial in the Kennedy pathway for phospholipid synthesis. It is not an approved drug but is extensively used in laboratory research to study membrane biogenesis, signal transduction, metabolic diseases, and cholinergic signaling. |
| Molecular Formula |
C11H20N4O11P2
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|---|---|
| Molecular Weight |
478.24300
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| Exact Mass |
478.05
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| CAS # |
3036-18-8
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| PubChem CID |
123727
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| Appearance |
White to off-white solid powder
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| Density |
2.13g/cm3
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| Boiling Point |
736ºC at 760mmHg
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| Flash Point |
398.9ºC
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| Index of Refraction |
1.736
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| LogP |
-7.2
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
28
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| Complexity |
746
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C1=CN(C(=O)N=C1N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)OCCN)O)O
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| InChi Key |
WVIMUEUQJFPNDK-PEBGCTIMSA-N
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| InChi Code |
InChI=1S/C11H20N4O11P2/c12-2-4-23-27(19,20)26-28(21,22)24-5-6-8(16)9(17)10(25-6)15-3-1-7(13)14-11(15)18/h1,3,6,8-10,16-17H,2,4-5,12H2,(H,19,20)(H,21,22)(H2,13,14,18)/t6-,8-,9-,10-/m1/s1
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| Chemical Name |
[2-aminoethoxy(hydroxy)phosphoryl] [(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0910 mL | 10.4550 mL | 20.9100 mL | |
| 5 mM | 0.4182 mL | 2.0910 mL | 4.1820 mL | |
| 10 mM | 0.2091 mL | 1.0455 mL | 2.0910 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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