| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Cyanine7 DBCO is not a pharmaceutical drug and does not target a biological receptor. Instead, the DBCO group specifically targets azide (-N3) groups on azide-functionalized biomolecules via copper-free, strain-promoted alkyne-azide cycloaddition (SPAAC). The reaction proceeds rapidly at room temperature without the need for a cytotoxic copper catalyst. The Cy7 fluorophore serves as a detection label, enabling visualization and tracking of azide-tagged molecules in biological systems. This compound is a chemical tool for bioconjugation and imaging applications.
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| ln Vitro |
In vitro, Cyanine7 DBCO is used to label azide-modified biomolecules such as proteins, peptides, nucleic acids, glycans, and lipids via copper-free click chemistry. The DBCO group reacts with azides at room temperature in aqueous buffers (PBS, pH 7.4) without copper, avoiding the cytotoxicity associated with CuAAC. The near-infrared fluorescence of Cyanine7 (excitation/emission ~750/773 nm) allows for reduced background autofluorescence, deeper tissue penetration, and minimal phototoxicity, making it ideal for live-cell imaging. The labeling reaction is complete within 30-120 minutes. Labeled biomolecules retain their biological activity and can be visualized by fluorescence microscopy or flow cytometry.
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| ln Vivo |
In vivo, Cyanine7 DBCO is used as a bioorthogonal labeling tool for near-infrared fluorescence imaging in live animals. The DBCO group enables rapid, copper-free conjugation to azide-functionalized targeting ligands (e.g., antibodies, peptides, nanoparticles), and the Cyanine7 NIR fluorophore allows for deep tissue penetration (up to 1-2 cm) with minimal autofluorescence. The water solubility and high photostability of Cyanine7 DBCO make it ideal for tracking the biodistribution of azide-tagged molecules in real time in living mice, for fluorescence-guided surgery, and for studying molecular interactions in complex biological environments. The imaging depth and sensitivity are superior to visible-wavelength dyes.
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| Enzyme Assay |
For non-cell-based click conjugation assays, a standard protocol uses strain-promoted alkyne-azide cycloaddition (SPAAC). The azide-functionalized biomolecule (0.5-1 mg/mL) is mixed with a 1.2-5 fold molar excess of Cyanine7 DBCO in PBS buffer (pH 7.4) or 100 mM sodium phosphate buffer (pH 7.4) containing 5-10% DMSO if necessary. The reaction is incubated at room temperature for 1-2 hours, protected from light. The reaction progress can be monitored by SDS-PAGE followed by fluorescence scanning (Ex 750 nm, Em 780 nm) or by LC-MS. Unconjugated dye is removed by size exclusion chromatography (Sephadex G-25, Zeba spin column) or by dialysis. The labeling degree is determined by measuring absorbance at 280 nm (protein) and 750 nm (dye) using a spectrophotometer and the manufacturer's correction factor.
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| Cell Assay |
For in vitro cell-based assays, cells are first metabolically labeled with an azide-modified sugar (e.g., Ac4GalNAz for glycan labeling) or incubated with an azide-functionalized antibody or targeting ligand. Cells are seeded in glass-bottom culture dishes and cultured for 24 hours. For metabolic labeling, cells are incubated with 10-100 uM azide-sugar in culture medium for 48-72 hours. For direct labeling, cells are incubated with azide-functionalized antibody (10 ug/mL) for 30 minutes at 4degC. Cells are then washed and fixed with 4% paraformaldehyde for 15 minutes at room temperature (for fixed-cell imaging) or used live. Fixed cells are incubated with 10-20 uM Cyanine7 DBCO in PBS for 30-60 minutes at room temperature. For live-cell labeling, cells are incubated with 10-20 uM Cyanine7 DBCO in serum-free medium for 30 minutes at 37degC. After washing, cells are imaged using a confocal microscope with 750 nm excitation and 780 nm emission filters.
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| Animal Protocol |
NIR fluorescence imaging in live animals: Female BALB/c nude mice (6-8 weeks old) bearing subcutaneous tumor xenografts (100-200 mm3) are used. An azide-functionalized tumor-targeting antibody (e.g., anti-EGFR antibody) is first conjugated to Cyanine7 DBCO via SPAAC in PBS for 2 hours at room temperature. The labeled antibody is purified and then administered intravenously via tail vein injection at 100-200 ug per mouse in 200 uL PBS. Mice are anesthetized with 2% isoflurane. Whole-body fluorescence images are acquired at 0, 2, 6, 12, 24, 48, and 72 hours post-injection using an in vivo imaging system equipped with 745 nm excitation and 800 nm emission filters. The fluorescence intensity in the tumor and major organs (liver, kidney, spleen, lung, heart) is quantified. The mice are euthanized at the final time point, and organs are harvested for ex vivo imaging to confirm the biodistribution.
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| ADME/Pharmacokinetics |
Cyanine7 DBCO has a molecular weight of 995.13 g/mol and a molecular formula of C58H65F6N4O2P. The compound exhibits high water solubility (water-soluble NIR fluorescent dye), high extinction coefficient, and good photostability. It is soluble in DMSO and water. Stock solutions (10 mM) are prepared in DMSO. Working solutions (0.5-5 uM for labeling) are prepared in PBS or cell culture medium. The compound should be stored at -20degC, protected from light and moisture. In solution, it is stable for 6 months at -80degC or 1 month at -20degC when sealed and protected from light. The DBCO group is stable in aqueous buffers at pH 6-8 for several hours, but prolonged storage in solution may lead to hydrolysis.
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| Toxicity/Toxicokinetics |
As a fluorescent probe for research use, Cyanine7 DBCO is not intended for human therapeutic or diagnostic applications. No specific toxicity data is available. The compound should be handled as a potential irritant; standard chemical safety precautions (gloves, lab coat, safety goggles) should be followed. The DBCO group is considered bioorthogonal and non-toxic at labeling concentrations. In cell culture, the compound is used at concentrations of 5-20 uM with no significant cytotoxicity observed. In animal studies, labeled antibodies or conjugates are generally well tolerated. The compound is light-sensitive and should be protected from light to prevent photobleaching.
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| References | |
| Additional Infomation |
Cyanine7 DBCO is a versatile bioorthogonal labeling reagent for near-infrared fluorescence imaging. The DBCO group allows for copper-free click chemistry, which is essential for live-cell and in vivo applications where copper would be toxic. The Cyanine7 NIR fluorophore provides deep tissue penetration and minimal background autofluorescence, enabling non-invasive imaging in living animals. This compound is widely used in chemical biology, glycan imaging, and preclinical imaging studies to track azide-functionalized molecules in real time. It is particularly valuable for fluorescence-guided surgery, tumor imaging, and drug delivery research. This product is for research use only.
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| Molecular Formula |
C58H65F6N4O2P
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| Molecular Weight |
995.126497030258
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| Exact Mass |
994.474
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| CAS # |
2692677-77-1
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| PubChem CID |
164577390
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| Appearance |
Pale purple to purple solid powder
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
71
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| Complexity |
1940
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| Defined Atom Stereocenter Count |
0
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| SMILES |
P(F)(F)(F)(F)F.C([N+]1=C(C(C)(C)C2=CC=CC=C12)C=CC1CCC/C(=C\C=C2\N(C3=CC=CC=C3C\2(C)C)C)/C=1)CCCCC(=O)NCCCCCC(N1CC2=CC=CC=C2C#CC2=CC=CC=C12)=O.[F-] |t:19|
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| InChi Key |
BCPAGJWDXBIMJR-UHFFFAOYSA-O
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| InChi Code |
InChI=1S/C58H64N4O2.F6P/c1-57(2)48-26-13-16-29-51(48)60(5)53(57)37-33-43-21-20-22-44(41-43)34-38-54-58(3,4)49-27-14-17-30-52(49)61(54)40-19-7-8-31-55(63)59-39-18-6-9-32-56(64)62-42-47-25-11-10-23-45(47)35-36-46-24-12-15-28-50(46)62;1-7(2,3,4,5)6/h10-17,23-30,33-34,37-38,41H,6-9,18-22,31-32,39-40,42H2,1-5H3;/q;-1/p+1
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| Chemical Name |
N-[6-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-6-oxohexyl]-6-[3,3-dimethyl-2-[2-[3-[2-(1,3,3-trimethylindol-1-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]indol-1-yl]hexanamide;hexafluorophosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0049 mL | 5.0245 mL | 10.0489 mL | |
| 5 mM | 0.2010 mL | 1.0049 mL | 2.0098 mL | |
| 10 mM | 0.1005 mL | 0.5024 mL | 1.0049 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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