| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
CY5-YNE does not have a defined biological target in the traditional pharmacological sense. Its primary application is as a fluorescent labeling reagent for biomolecules. It is designed to react with azide groups via click chemistry, enabling the attachment of the CY5 fluorophore to target molecules. The dye itself is used for detection and imaging purposes rather than for modulating biological activity.
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|---|---|
| ln Vitro |
In vitro, CY5-YNE is used as a reactive dye for labeling amino groups in peptides, proteins, and oligonucleotides. It is a click chemistry reagent that can undergo CuAAc with molecules containing azide groups. Its primary activity is as a labeling tool for fluorescence-based detection, with no reported direct biological activity such as enzyme inhibition or receptor modulation.
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| ln Vivo |
In vivo activity of CY5-YNE is not typically characterized, as the compound is used as a labeling reagent for in vitro and ex vivo applications. It may be used in vivo imaging studies when conjugated to targeting molecules, but its intrinsic pharmacological activity is not relevant to its function as a fluorescent probe.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable to CY5-YNE, as it is not a bioactive molecule designed to interact with biological targets. The compound is a chemical reagent used for labeling and detection. Its reactivity is with azide groups in click chemistry, not with enzymes or receptors.
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| Cell Assay |
In vitro cell-based assays for CY5-YNE typically involve staining cells or cellular components for fluorescence microscopy or flow cytometry. The dye can be used to label proteins or other biomolecules of interest, allowing for their visualization and quantification. Cells are incubated with the labeled molecule, and fluorescence is detected using appropriate instrumentation.
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| Animal Protocol |
In vivo animal studies are not typically performed with CY5-YNE itself, as it is a labeling reagent. However, it may be used in vivo imaging studies when conjugated to a targeting ligand or biomolecule. The labeled molecule would be administered to animals, and its distribution and accumulation would be monitored by fluorescence imaging.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) properties of CY5-YNE are not typically characterized, as it is not a therapeutic agent. The compound is a chemical reagent used for labeling. It is soluble in DMSO at ≥43 mg/mL and should be protected from light during storage.
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| Toxicity/Toxicokinetics |
The toxicity profile of CY5-YNE has not been characterized in preclinical studies. The compound is intended for research use only and is not approved for human therapeutic applications. Standard safety precautions for handling fluorescent dyes should be followed.
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| Additional Infomation |
CY5-YNE is a research-grade fluorescent dye used in various applications, including fluorescence microscopy, flow cytometry, and in vivo imaging. It is also known as Sulfo-Cyanine5-alkyne. The compound is supplied as a powder with a purity of ≥98% and should be stored at 4°C or -20°C, protected from light.
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| Molecular Formula |
C36H43N3O7S2
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|---|---|
| Molecular Weight |
693.872527360916
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| Exact Mass |
693.254
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| CAS # |
1345823-20-2
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| PubChem CID |
122129477
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| Appearance |
Grayish brown to reddish brown solid powder
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| LogP |
7.571
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
48
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| Complexity |
1570
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCN\1C2=C(C=C(C=C2)S(=O)(=O)[O-])C(/C1=C\C=C\C=C\C3=[N+](C4=C(C3(C)C)C=C(C=C4)S(=O)(=O)O)CCCCCC(=O)NCC#C)(C)C
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| InChi Key |
SDCASIAEYVOHMO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C36H43N3O7S2/c1-7-22-37-34(40)17-13-10-14-23-39-31-21-19-27(48(44,45)46)25-29(31)36(5,6)33(39)16-12-9-11-15-32-35(3,4)28-24-26(47(41,42)43)18-20-30(28)38(32)8-2/h1,9,11-12,15-16,18-21,24-25H,8,10,13-14,17,22-23H2,2-6H3,(H2-,37,40,41,42,43,44,45,46)
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| Chemical Name |
(2E)-2-[(2E,4E)-5-[3,3-dimethyl-1-[6-oxo-6-(prop-2-ynylamino)hexyl]-5-sulfoindol-1-ium-2-yl]penta-2,4-dienylidene]-1-ethyl-3,3-dimethylindole-5-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 43 mg/mL (~61.97 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4412 mL | 7.2060 mL | 14.4119 mL | |
| 5 mM | 0.2882 mL | 1.4412 mL | 2.8824 mL | |
| 10 mM | 0.1441 mL | 0.7206 mL | 1.4412 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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