| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
CY3 is a fluorescent dye and does not have a specific pharmacological target. It is used as a labeling reagent for biological molecules. The dye conjugates easily to proteins and oligonucleotides for labeling purposes. It is an indocyanine dye with high fluorescence.
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|---|---|
| ln Vitro |
CY3 is a fluorescent marker with high brightness and photostability. It is commonly used for fluorescent labeling of proteins and nucleic acids. The dye has an excitation maximum of 554 nm and an emission maximum of 568 nm. It is an amine-reactive cyanine orange dye.
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| ln Vivo |
CY3 is used in biological research for fluorescent labeling of proteins and nucleic acids. It is not a drug and has no direct in vivo activity. It is used as a research tool for imaging and detection applications.
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| Enzyme Assay |
CY3 is characterized in cell-free systems for its photophysical properties. Absorption and emission spectra are measured using UV-Vis and fluorescence spectroscopy. Fluorescence quantum yield and brightness are determined. The dye's reactivity with amines is assessed using standard bioconjugation techniques.
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| Cell Assay |
CY3 is used in cell-based assays for fluorescent labeling of proteins and nucleic acids. Cells are incubated with CY3-conjugated probes, and fluorescence imaging is performed to visualize the localization and expression of target molecules. Cellular uptake and specificity are assessed.
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| Animal Protocol |
CY3 is used in animal models for in vivo imaging applications. Fluorescence imaging is performed to detect CY3-labeled probes in tissues. The probe's biodistribution, targeting, and clearance are evaluated. However, specific animal study protocols are not extensively documented.
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| ADME/Pharmacokinetics |
CY3 has a molecular weight of 911.01 and a molecular formula of C43H50N4O14S2. It has a CAS number of 146397-20-8. The compound is a purple to purplish red solid powder with a purity of ≥98%. It has a LogP of 7.794.
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| Toxicity/Toxicokinetics |
CY3 is a fluorescent dye and is not intended for human therapeutic use. No detailed toxicity data is available. The compound is for research use only.
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| Additional Infomation |
Cy3 bifunctional dye zwitterion is a Cy3 dye and imine betaine. It is the conjugate acid of Cy3 bifunctional dye (1-).
CY3 is a traditional fluorescent marker for proteins and nucleic acids. It has a CAS number of 146397-20-8. It is also known as Cy 3 Non-Sulfonated and Cyanine3. It is an indocyanine dye with high fluorescence. The compound is for research use only and has not been approved for clinical use. |
| Molecular Formula |
C43H50N4O14S2
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|---|---|
| Molecular Weight |
911.01
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| Exact Mass |
910.276
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| CAS # |
146397-20-8
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| Related CAS # |
Cy 3 (Non-Sulfonated) (triethylamine)
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| PubChem CID |
135401967
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| Appearance |
Purple to purplish red solid powder
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| LogP |
7.794
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
19
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| Heavy Atom Count |
63
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| Complexity |
2110
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=S(C1=CC2=C([N+](CCCCCC(ON3C(CCC3=O)=O)=O)=C(/C=C/C=C4N(CCCCCC(ON5C(CCC5=O)=O)=O)C6=C(C=C(S(=O)(O)=O)C=C6)C/4(C)C)C2(C)C)C=C1)([O-])=O
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| InChi Key |
OHOQEZWSNFNUSY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C43H50N4O14S2/c1-42(2)30-26-28(62(54,55)56)16-18-32(30)44(24-9-5-7-14-40(52)60-46-36(48)20-21-37(46)49)34(42)12-11-13-35-43(3,4)31-27-29(63(57,58)59)17-19-33(31)45(35)25-10-6-8-15-41(53)61-47-38(50)22-23-39(47)51/h11-13,16-19,26-27H,5-10,14-15,20-25H2,1-4H3,(H-,54,55,56,57,58,59)
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| Chemical Name |
1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-2-[(E,3E)-3-[1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-5-sulfoindol-2-ylidene]prop-1-enyl]-3,3-dimethylindol-1-ium-5-sulfonate
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| Synonyms |
CY 3 CY-3 Cy3-bifunctional dye zwitterion
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~41.67 mg/mL (~45.74 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0977 mL | 5.4884 mL | 10.9768 mL | |
| 5 mM | 0.2195 mL | 1.0977 mL | 2.1954 mL | |
| 10 mM | 0.1098 mL | 0.5488 mL | 1.0977 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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