| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
| Targets |
The primary use of Crystal Ponceau 6R is as a staining reagent in histology, where it binds to fibrin in tissue sections. It does not target a specific biological receptor but rather stains proteins and other tissue components through electrostatic and hydrophobic interactions. It is used in the MSB Trichrome stain to differentiate fibrin from other tissue elements.
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|---|---|
| ln Vitro |
In vitro, Crystal Ponceau 6R is used as a staining reagent in histological protocols. Tissue sections are incubated with the dye solution, which binds to fibrin and other tissue components. The stained sections are then examined under a microscope to identify fibrin deposits. The compound is also used in food industry applications as a colorant.
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| ln Vivo |
Crystal Ponceau 6R is not typically used in vivo for therapeutic or diagnostic purposes. It is primarily used in vitro as a staining reagent. The compound may be used in ex vivo applications, such as staining tissue sections, but it is not administered to living organisms for systemic effects.
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| Enzyme Assay |
In vitro, Crystal Ponceau 6R is used in the MSB Trichrome stain protocol. Tissue sections are deparaffinized, rehydrated, and then incubated in the dye solution. The sections are then washed and differentiated in acidic solutions before being counterstained and mounted for microscopic examination. The dye stains fibrin bright red, allowing for its identification in tissue sections.
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| Animal Protocol |
Crystal Ponceau 6R is not typically used in in vivo animal experiments. It is primarily used as an in vitro staining reagent. The compound may be used in ex vivo applications, such as staining tissue sections from animal models, but it is not administered to living animals for systemic effects.
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| ADME/Pharmacokinetics |
Crystal Ponceau 6R has a molecular weight of 502.43 and the chemical formula C₂₀H₁₂N₂Na₂O₇S₂. It is a red azo dye that is soluble in water. The compound is supplied as a powder and should be stored in a sealed container in a cool, dry place, protected from light. It is for research use only and not for human therapeutic use.
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| Toxicity/Toxicokinetics |
Specific toxicity data for Crystal Ponceau 6R are not extensively documented. As a synthetic azo dye, it may be irritating to the skin, eyes, and respiratory tract. Some azo dyes are known to be carcinogenic, although the specific toxicity of this compound has not been fully characterized. The compound is for research use only and not for human therapeutic use. It should be handled with appropriate safety precautions.
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| References | |
| Additional Infomation |
Acid Red 44 is an organosodium salt, the disodium salt of 7-hydroxy-8-[(naphthyl-1-yl)diazotano]naphthalene-1,3-disulfonic acid. It is used as a histological dye. It contains 7-hydroxy-8-[(naphthyl-1-yl)diazotano]naphthalene-1,3-disulfonic acid.
Crystal Ponceau 6R is also known as C.I. Acid Red 44. It is a red azo dye used in histology for staining fibrin with the MSB Trichrome stain. It is used in the food industry as a colorant. It has a molecular weight of 502.43. It is supplied as a powder and should be stored in a cool, dry place. It is for research use only. |
| Molecular Formula |
C20H14N2O7S2.NA+
|
|---|---|
| Molecular Weight |
481.45416
|
| Exact Mass |
501.988
|
| CAS # |
2766-77-0
|
| PubChem CID |
165025
|
| Appearance |
Brown to reddish brown solid powder
|
| Melting Point |
>300ºC(lit.)
|
| LogP |
6.083
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
33
|
| Complexity |
854
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
FUGCXLNGEHFIOA-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C20H14N2O7S2.2Na/c23-17-9-8-13-10-14(30(24,25)26)11-18(31(27,28)29)19(13)20(17)22-21-16-7-3-5-12-4-1-2-6-15(12)16;;/h1-11,23H,(H,24,25,26)(H,27,28,29);;/q;2*+1/p-2
|
| Chemical Name |
disodium;7-hydroxy-8-(naphthalen-1-yldiazenyl)naphthalene-1,3-disulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0771 mL | 10.3853 mL | 20.7706 mL | |
| 5 mM | 0.4154 mL | 2.0771 mL | 4.1541 mL | |
| 10 mM | 0.2077 mL | 1.0385 mL | 2.0771 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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