| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
|
||
| Other Sizes |
| Targets |
Crocein Scarlet 7B does not target a specific biological receptor; it is a chemical dye used for staining. It binds to biological molecules such as proteins and nucleic acids through electrostatic and hydrophobic interactions, allowing for visualization in histological and cytological preparations. It is also used to detect bloodstain fingerprints by staining the protein components of blood.
|
|---|---|
| ln Vitro |
In vitro, Crocein Scarlet 7B is used as a stain in hematology and histology. It is used to stain blood cells, tissues, and other biological samples for microscopic examination. It is also used in the preparation of bacterial stains for microbiology. The dye binds to proteins and other cellular components, providing contrast for visualization.
|
| ln Vivo |
In vivo, Crocein Scarlet 7B is not typically used for therapeutic or diagnostic purposes. It is primarily used in vitro as a staining reagent. The compound may be used in ex vivo applications, such as staining tissue sections or blood smears, but it is not administered to living organisms for systemic effects.
|
| Enzyme Assay |
In vitro, Crocein Scarlet 7B is used as a staining reagent in histological and cytological protocols. Biological samples, such as tissue sections or blood smears, are incubated with the dye solution for a specified time. The samples are then washed and examined under a microscope. The dye binds to proteins and other cellular components, providing contrast for visualization. The protocol may vary depending on the specific application and the type of sample.
|
| Animal Protocol |
Crocein Scarlet 7B is not typically used in in vivo animal experiments. It is primarily used as an in vitro staining reagent. The compound may be used in ex vivo applications, such as staining tissue sections from animal models, but it is not administered to living animals for systemic effects.
|
| ADME/Pharmacokinetics |
Crocein Scarlet 7B has a molecular weight of 584.54 and the chemical formula C₂₄H₁₈N₄Na₂O₇S₂. It is a synthetic azo dye that is soluble in water. The compound is supplied as a powder and should be stored in a sealed container in a cool, dry place, protected from light. It is for research use only and not for human therapeutic use.
|
| Toxicity/Toxicokinetics |
Specific toxicity data for Crocein Scarlet 7B are not extensively documented. As a synthetic azo dye, it may be irritating to the skin, eyes, and respiratory tract. Some azo dyes are known to be carcinogenic, although the specific toxicity of this compound has not been fully characterized. The compound is for research use only and not for human therapeutic use. It should be handled with appropriate safety precautions, including the use of personal protective equipment.
|
| References | |
| Additional Infomation |
Crocein Scarlet 7B is also known as C.I. Acid Red 44. It is a chemical dye used to detect bloodstain fingerprints. It is a synthetic azo dye used in hematology and histology. It is used in the preparation of bacterial stains. It is a red azo dye with a molecular weight of 584.54. It is supplied as a powder and should be stored in a cool, dry place. It is for research use only.
|
| Molecular Formula |
C24H20N4O7S2.NA
|
|---|---|
| Molecular Weight |
563.558
|
| Exact Mass |
584.041
|
| CAS # |
6226-76-2
|
| PubChem CID |
136054023
|
| Appearance |
Typically exists as solid at room temperature
|
| Melting Point |
181-188℃
|
| LogP |
7.962
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
11
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
39
|
| Complexity |
1030
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[NaH].CC1C=C(/N=N/C2C=CC(S(=O)(O)=O)=CC=2C)C=CC=1N/N=C1\C(=O)C=CC2=C\1C(S(=O)(O)=O)=CC=C2
|
| InChi Key |
GMWXFMFHCCFVJU-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C24H20N4O7S2.2Na/c1-14-12-17(25-26-20-10-8-18(13-15(20)2)36(30,31)32)7-9-19(14)27-28-24-21(29)11-6-16-4-3-5-22(23(16)24)37(33,34)35;;/h3-13,29H,1-2H3,(H,30,31,32)(H,33,34,35);;/q;2*+1/p-2
|
| Chemical Name |
disodium;7-hydroxy-8-[[2-methyl-4-[(2-methyl-4-sulfonatophenyl)diazenyl]phenyl]diazenyl]naphthalene-1-sulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7744 mL | 8.8722 mL | 17.7443 mL | |
| 5 mM | 0.3549 mL | 1.7744 mL | 3.5489 mL | |
| 10 mM | 0.1774 mL | 0.8872 mL | 1.7744 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
