| Size | Price | Stock | Qty |
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| 500mg |
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| Other Sizes |
| Targets |
Cresyl Violet acetate does not target a specific biological receptor or enzyme. Instead, it functions as a histological and fluorescent stain that binds to nucleic acids and other cellular components. Its fluorescence properties make it useful for imaging applications in neuroscience and pathology. The dye can also be used to study cellular morphology and neuronal degeneration.
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| ln Vitro |
In sections stained with PV and cresyl violet acetate, the estimated total number of SG neurons was 27,485±3251 and 26,705±1823, respectively. Between them, there is no discernible difference (p=0.552). For this reason, when assessing neuronal numbers, cresyl violet acetate staining is more straightforward and economical. PV is a laborious technique that restricts its quantitative application even if it gives functional status and staining spiral ganglion neurons (SGN) with increased intensity. Using the probe to determine the overall RC volume, the average RC volume in sections immunostained with PV and cresyl violet acetate was 1.82±0.33 mm3 and 2.162±0.35 mm3, respectively. Using nucleator probes, the volume of neurons was calculated to be 3487.63±951 μm3 in sections stained with PV immunostained tissue and 3740.1±784 μm3 in sections stained with cresyl violet acetate. The volumes of neuronal nuclei in sections stained with PV immunostained and cresyl violet acetate were determined to be 126.51±33 μm3 and 131.68±50 μm3, respectively, according to research using nucleation probes [1].
In vitro, Cresyl Violet acetate is used as a staining agent for various biological specimens. It exhibits red fluorescence when bound to cellular structures, allowing for visualization under a fluorescence microscope. It is soluble in water (1 mg/mL) and has a pH of approximately 3 (10 g/L, H₂O, 20°C). The compound is also used in cell culture applications with low endotoxin levels. |
| ln Vivo |
In vivo, Cresyl Violet acetate is not typically used as a therapeutic agent but is employed in animal studies for histological examination of nervous tissue. It is used to stain neurons in tissue sections from experimental animals to assess neuronal health, degeneration, and morphology. Its fluorescent properties enable detailed imaging of brain and spinal cord structures in ex vivo tissue samples.
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| Enzyme Assay |
For in vitro staining protocols, Cresyl Violet acetate is typically prepared as a 0.1-1% solution in water or buffer. Tissue sections or cells are incubated with the stain for 1-10 minutes, depending on the desired intensity. Excess stain is removed by washing with distilled water or buffer. Stained samples are then dehydrated, cleared, and mounted for microscopic examination.
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| Cell Assay |
For in vitro cell-based assays, Cresyl Violet acetate is used to stain cultured neurons or other cell types. Cells are grown on coverslips or chamber slides, fixed with paraformaldehyde or methanol, and incubated with the dye solution. After washing, cells are examined under a fluorescence microscope using appropriate excitation/emission filters. The stain can also be used in combination with other fluorescent markers for multiplex imaging.
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| Animal Protocol |
For in vivo animal experiments, Cresyl Violet acetate is used for post-mortem histological analysis. Animals are euthanized, and tissues (such as brain or spinal cord) are harvested, fixed, embedded in paraffin, and sectioned. Tissue sections are deparaffinized, rehydrated, and stained with Cresyl Violet acetate. Stained sections are then examined under a microscope to assess neuronal morphology and pathology.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Cresyl Violet acetate are not applicable, as it is not used as a therapeutic agent. The compound is a histological stain and is not administered systemically for pharmacological purposes. Its stability in solution has rarely been reported, and it is typically prepared fresh for staining applications.
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| Toxicity/Toxicokinetics |
Toxicological data for Cresyl Violet acetate are limited. As a synthetic dye, it may cause irritation upon contact with skin or eyes. It is incompatible with strong oxidizing agents. The compound is classified as a research-grade reagent and is not for human therapeutic use. Standard laboratory safety practices should be followed when handling the dye.
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| References | |
| Additional Infomation |
Cresyl Violet acetate is a research-use only compound and has not been approved for clinical applications. It is also known as cresyl violet acetate or CVA. Its molecular weight is 321.33, and its formula is C₁₈H₁₅N₃O₃. The compound has a melting point of 140-143°C. It is available from various research chemical suppliers for histological and fluorescent staining applications.
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| Molecular Formula |
C16H11N3O.C2H4O2
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| Molecular Weight |
321.33
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| Exact Mass |
321.111
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| CAS # |
10510-54-0
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| Related CAS # |
Cresyl Violet perchlorate;41830-80-2
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| PubChem CID |
82686
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| Appearance |
Brown to black solid powder
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| Boiling Point |
468.3ºC
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| Melting Point |
140-143 °C(lit.)
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| Flash Point |
237ºC
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| Vapour Pressure |
6.07E-09mmHg at 25°C
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| LogP |
3.919
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
24
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| Complexity |
522
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
UEWSHLFAYRXNHZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H11N3O.C2H4O2/c17-9-5-6-13-14(7-9)20-15-8-12(18)10-3-1-2-4-11(10)16(15)19-13;1-2(3)4/h1-8,18H,17H2;1H3,(H,3,4)
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| Chemical Name |
(9-aminobenzo[a]phenoxazin-5-ylidene)azanium;acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~1 mg/mL (~3.11 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1121 mL | 15.5603 mL | 31.1207 mL | |
| 5 mM | 0.6224 mL | 3.1121 mL | 6.2241 mL | |
| 10 mM | 0.3112 mL | 1.5560 mL | 3.1121 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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