| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg | |||
| Other Sizes |
| Targets |
Coumarin hydrazine functions as a fluorescent chemical probe that targets carbonyl groups on proteins and lipids. Its hydrazine moiety reacts with aldehydes or ketones to form hydrazones, enabling the fluorescent labeling of carbonylated biomolecules. At neutral pH, coumarin hydrazine reacts faster with aldehydes than coumarin hydrazide. The compound is used as a fluorogenic sensor in living cells to detect carbonylated biomolecules produced following oxidative stress. This makes it a valuable tool for studying oxidative stress and its effects on cellular biomolecules.
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| ln Vitro |
Coumarin hydrazine exhibits fluorescent properties with excitation at 420-450 nm and emission at 468 nm. It functions as a fluorescent chemical probe for labeling intracellular protein- and lipid-bound carbonyl groups. The compound's hydrazine moiety reacts with aldehydes or ketones to form hydrazones, enabling the detection of carbonylated biomolecules. It acts as a fluorogenic sensor in live cells for the detection of carbonylated biomolecules produced following oxidative stress. Coumarin hydrazine has potential antimicrobial, antioxidant, and anticancer properties.
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| ln Vivo |
In vivo activity data for coumarin hydrazine are limited. The compound is primarily used as a fluorescent probe for in vitro applications, including live cell imaging. It has been studied for its role in drug design, particularly in the development of bioactive molecules. The compound's ability to detect carbonylated biomolecules makes it useful for studying oxidative stress in biological systems. However, specific in vivo efficacy studies for coumarin hydrazine are not well-documented in the public domain. The compound is generally used for research applications in cell biology and biochemistry.
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| Enzyme Assay |
The in vitro enzyme/receptor binding assay for coumarin hydrazine typically involves incubating the compound with aldehyde- or ketone-containing biomolecules or model compounds in buffer solutions at neutral pH. The reaction between the hydrazine moiety and the carbonyl group forms hydrazones, which can be monitored by fluorescence spectroscopy (excitation 420-450 nm, emission 468 nm). The reaction kinetics can be assessed by measuring the increase in fluorescence over time. The compound can also be used to label proteins or lipids containing carbonyl groups, followed by analysis using SDS-PAGE or other separation techniques.
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| Cell Assay |
In vitro cellular assays for coumarin hydrazine typically involve treating cultured cells (such as HeLa or other cell lines) with the compound at concentrations ranging from 1 to 50 μM for 30 minutes to several hours. The cells are then washed and imaged using fluorescence microscopy with appropriate excitation and emission filters (excitation 420-450 nm, emission 468 nm) to detect carbonylated biomolecules. Oxidative stress can be induced in cells using agents such as hydrogen peroxide or paraquat to increase the production of carbonylated biomolecules. The compound is dissolved in DMSO as a stock solution and diluted in cell culture medium.
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| Animal Protocol |
Coumarin hydrazine is primarily used for in vitro and cell-based studies, and in vivo animal studies are not extensively documented. For potential in vivo applications, the compound could be administered to mice via intravenous or intraperitoneal injection, and tissue samples could be collected for fluorescence imaging or analysis. The compound's fluorescence properties (excitation 420-450 nm, emission 468 nm) would allow for detection in biological tissues. However, specific protocols for in vivo animal studies with coumarin hydrazine are not well-established in the public domain.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of coumarin hydrazine are not extensively documented. The compound has a molecular weight of 190.20 g/mol, which is favorable for cellular uptake. It has an XLogP3 value of 1.5, indicating moderate lipophilicity. The compound has a hydrogen bond donor count of 2 and acceptor count of 4. It is typically stored at -20°C. Detailed PK parameters such as half-life, bioavailability, and tissue distribution would require experimental determination. The compound is primarily used as a research tool for fluorescent labeling applications.
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| Toxicity/Toxicokinetics |
Toxicity data for coumarin hydrazine are limited. The compound is intended for research use only and is not approved for human therapeutic applications. Cellular toxicity studies have suggested that coumarin-based fluorescent probes can be used to monitor hydrazine in live cells with acceptable toxicity profiles. However, as with all chemical compounds, appropriate safety precautions should be taken when handling. Standard toxicity assessments would include acute toxicity, genotoxicity, and repeated-dose toxicity studies. The compound's hydrazine moiety may contribute to potential toxicity at high concentrations.
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| References | |
| Additional Infomation |
Coumarin hydrazine (CAS 113707-87-2) is an aromatic hydrazine-containing fluorophore used for labeling aldehydes or ketones. It acts as a fluorogenic sensor in live cells to detect carbonylated biomolecules that occur following oxidative stress. The compound exhibits excitation at 420-450 nm and emission at 468 nm. It reacts with aldehydes or ketones to form hydrazones for fluorescent labeling. Coumarin hydrazine is used as a fluorescent chemical probe for labeling intracellular protein- and lipid-bound carbonyl groups. It is also known as 7-hydrazinyl-4-methyl-2H-chromen-2-one. The compound is not approved for clinical use and is intended for research applications.
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| Molecular Formula |
C10H10N2O2
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|---|---|
| Molecular Weight |
190.198602199554
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| Exact Mass |
190.074
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| CAS # |
113707-87-2
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| PubChem CID |
13901808
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
1.5
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
14
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| Complexity |
275
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC(=O)OC2=C1C=CC(=C2)NN
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| InChi Key |
ZGALTRTXIDNBJL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H10N2O2/c1-6-4-10(13)14-9-5-7(12-11)2-3-8(6)9/h2-5,12H,11H2,1H3
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| Chemical Name |
7-hydrazinyl-4-methylchromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMF : 12.5 mg/mL (~65.72 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2576 mL | 26.2881 mL | 52.5762 mL | |
| 5 mM | 1.0515 mL | 5.2576 mL | 10.5152 mL | |
| 10 mM | 0.5258 mL | 2.6288 mL | 5.2576 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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