| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Coumarin 6 does not have a specific pharmacological target. As a fluorescent dye, it is used as a probe for labeling and tracking purposes rather than for modulating biological pathways. The compound is used to stain organelles and other cellular structures. It is used as a fluorescent probe in microparticle drug delivery systems to conduct in vivo tracking, cell uptake, and transport mechanism studies of drug delivery systems. The dye's microenvironment sensitivity allows it to report on local changes in polarity, viscosity, and other physicochemical properties. Coumarin 6 is also used as a laser dye for blue-green spectrum applications.
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| ln Vitro |
HPMC nanoparticles tagged with coumarin 6 [3] (1) Prepare an organic phase solution containing 5 mg/mL by dissolving coumarin 6 in DMF. (2) HBSS-represented dissolved concentration supplemented cellulose (3) Using a MasterFlex L/S pump, add the organic solution to the aqueous solution (volume ratio: 1:100) at a 36 mL/min injection rate (4) Add 9 mL (5) of 2 μg/mL nanoparticles to the MDCKII cell culture dish and shield from light for five, fifteen, thirty, and sixty minutes. (6) To prevent MDCKII cell food nanoparticles, add cold PBS buffer and rinse with HBSS. (7) Examine the MDCKII cells' meal on nanoparticles under a fluorescent microscope.
In vitro, Coumarin 6 is used as a fluorescent probe to label and track various biological structures and materials. It is used to stain organelles and other cellular structures. The dye exhibits strong fluorescence with excitation and emission maxima at λex = 450 nm and λem = 505 nm, respectively. It is frequently used to facilitate the traceability of drug delivery systems in vitro. The compound is used as a fluorescent probe in microparticle drug delivery systems to study cell uptake and transport mechanisms. Its microenvironment sensitivity allows it to report on local changes in the cellular environment. The dye's stability and excellent performance in lifetime experiments make it valuable for fluorescence spectroscopy applications. |
| ln Vivo |
In vivo, Coumarin 6 is used as a fluorescent probe for tracking drug delivery systems. The dye is used in microparticle drug delivery systems to conduct in vivo tracking of drug distribution and release profiles. Its excitation and emission maxima at λex = 450 nm and λem = 505 nm make it an ideal marker for evaluating drug distribution and release profiles in biological systems. The compound can be used for fluorescence imaging of labeled materials in animal models. Its stability and fluorescence properties make it suitable for long-term tracking studies.
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| Enzyme Assay |
For non-cellular assays, Coumarin 6 is used as a fluorescent probe and laser dye. The compound's fluorescence properties can be characterized using spectrophotometry and spectrofluorometry. Its excitation and emission spectra are measured to confirm the λex = 450 nm and λem = 505 nm maxima. The dye can be used to calibrate fluorescence imaging microscopy systems. Its microenvironment sensitivity can be assessed by measuring fluorescence changes in different solvents or conditions. The compound's stability in lifetime experiments can be evaluated using time-resolved fluorescence spectroscopy.
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| Cell Assay |
For in vitro cell-based assays, cells are incubated with Coumarin 6 or Coumarin 6-labeled materials. Fluorescence is measured using flow cytometry or fluorescence microscopy at excitation/emission of 450/505 nm. The dye can be used to stain organelles and other cellular structures. For drug delivery studies, cells are treated with Coumarin 6-labeled nanoparticles or microparticles, and cellular uptake is assessed by measuring fluorescence intensity. The dye's fluorescence allows for visualization of labeled materials and assessment of their intracellular distribution.
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| Animal Protocol |
For in vivo imaging studies, Coumarin 6 or Coumarin 6-labeled materials are administered to animal models. Fluorescence imaging is performed at excitation/emission of 450/505 nm. The dye is used to track the distribution and release of drug delivery systems in vivo. Its fluorescence properties allow for non-invasive imaging of labeled materials.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Coumarin 6 include excitation and emission maxima at λex = 450 nm and λem = 505 nm, respectively. The compound has a dye content of 98%. It is soluble in appropriate organic solvents. Storage should be protected from light. The compound's stability makes it suitable for various fluorescence applications.
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| Toxicity/Toxicokinetics |
As a fluorescent dye, Coumarin 6 is generally considered to have low toxicity at the concentrations used for labeling and imaging applications. It is not intended for human use. No significant toxicity has been reported in standard research applications. The compound should be handled with standard laboratory precautions.
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| References |
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| Additional Infomation |
Coumarin 6 belongs to the 7-aminocoumarin family and functions as a fluorescent dye. It has been reported that coumarin 6 has been found in Ferula fukanensis, and relevant data are available for reference.
Coumarin 6 is a fluorescent dye belonging to the 7-diethylaminocoumarin series. It is a highly fluorescent probe and laser dye. The compound emits fluorescence in solid and solution states and is used as a blue-green spectrum laser dye. It is microenvironment sensitive. Coumarin 6 is frequently used to facilitate the traceability of drug delivery systems in vitro and in vivo. It is used as a fluorescent probe in microparticle drug delivery systems for in vivo tracking, cell uptake, and transport mechanism studies. The compound is also used to stain organelles and to calibrate fluorescence imaging microscopy systems. |
| Molecular Formula |
C20H18N2O2S
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|---|---|
| Molecular Weight |
350.4341
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| Exact Mass |
350.109
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| CAS # |
38215-36-0
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| PubChem CID |
100334
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| Appearance |
Yellow to orange solid powder
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| Density |
1.311 g/cm3
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| Boiling Point |
570.1ºC at 760 mmHg
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| Melting Point |
208-210 °C(lit.)
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| Flash Point |
298.6ºC
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| Index of Refraction |
1.69
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| LogP |
4.915
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
25
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| Complexity |
534
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VBVAVBCYMYWNOU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H18N2O2S/c1-3-22(4-2)14-10-9-13-11-15(20(23)24-17(13)12-14)19-21-16-7-5-6-8-18(16)25-19/h5-12H,3-4H2,1-2H3
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| Chemical Name |
3-(1,3-benzothiazol-2-yl)-7-(diethylamino)chromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~14.27 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8536 mL | 14.2682 mL | 28.5364 mL | |
| 5 mM | 0.5707 mL | 2.8536 mL | 5.7073 mL | |
| 10 mM | 0.2854 mL | 1.4268 mL | 2.8536 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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