| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Columbamine targets various molecular pathways. It has been shown to inhibit topoisomerase enzymes, modulate NF-κB signaling, and induce apoptosis in cancer cells. The compound also exhibits antibacterial activity against various Gram-positive and Gram-negative bacteria. It may interact with DNA and interfere with DNA replication and transcription.
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| ln Vitro |
In vitro, columbamine has been shown to inhibit the growth of various cancer cell lines and induce apoptosis. It also exhibits antibacterial activity against various pathogens. The compound has anti-inflammatory effects by reducing the production of pro-inflammatory cytokines and inhibiting the activation of NF-κB.
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| ln Vivo |
In vivo, columbamine has been studied in animal models of inflammation and cancer. It may have anti-tumor effects in xenograft models and anti-inflammatory effects in models of inflammation. Its pharmacokinetics and bioavailability are areas of active research.
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| Enzyme Assay |
For non-cellular enzyme assays, columbamine can be tested for inhibition of topoisomerase enzymes using standard enzyme activity assays. It can also be tested for antioxidant activity using DPPH, ABTS, or FRAP assays.
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| Cell Assay |
For in vitro cell-based assays, cancer cell lines and bacterial cultures are used to assess the biological activities of columbamine. Cell proliferation, apoptosis, and inflammatory marker expression are assessed.
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| Animal Protocol |
For in vivo animal studies, columbamine can be administered to animal models of disease. Inflammation models and cancer models can be used.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of columbamine include solubility in DMSO and ethanol. It is a lipophilic alkaloid and may have moderate oral bioavailability.
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| Toxicity/Toxicokinetics |
As a natural product, columbamine is generally considered safe at low doses. No significant toxicity has been reported in preclinical studies.
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| Additional Infomation |
Grumbamine is a berberine alkaloid and an organic heterotetracyclic compound. It has been reported to exist in Fibraurea chloroleuca, Corydalis ternata, and other organisms with relevant data.
Columbamine is a naturally occurring isoquinoline alkaloid. It has antibacterial, antifungal, anti-inflammatory, and anticancer activities. It is a member of the protoberberine family of alkaloids and is structurally related to berberine. |
| Molecular Formula |
C20H20NO4
|
|---|---|
| Molecular Weight |
338.3771
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| Exact Mass |
338.138
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| CAS # |
3621-36-1
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| Related CAS # |
Columbamine chloride;1916-10-5
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| PubChem CID |
72310
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| Appearance |
Light yellow to orange solid powder
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| LogP |
-1.89
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
25
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| Complexity |
461
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YYFOFDHQVIODOQ-UHFFFAOYSA-O
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| InChi Code |
InChI=1S/C20H19NO4/c1-23-18-5-4-12-8-16-14-10-17(22)19(24-2)9-13(14)6-7-21(16)11-15(12)20(18)25-3/h4-5,8-11H,6-7H2,1-3H3/p+1
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| Chemical Name |
3,9,10-trimethoxy-5,6-dihydroisoquinolino[2,1-b]isoquinolin-7-ium-2-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 23 mg/mL (~67.97 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9553 mL | 14.7763 mL | 29.5526 mL | |
| 5 mM | 0.5911 mL | 2.9553 mL | 5.9105 mL | |
| 10 mM | 0.2955 mL | 1.4776 mL | 2.9553 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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