| Size | Price | |
|---|---|---|
| 100mg | ||
| 250mg | ||
| 500mg |
| ln Vitro |
Clomipramine suppresses both norepinephrine and 5-HT reuptake, with clomipramine suppressing 5-HT reuptake more significantly than norepinephrine reuptake [1]. Clomipramine, an antidepressant, has no effect on AChE in the striatum of the rat brain, but it inhibits AChE in venom and human serum BChE in a concentration-dependent way [2]. Under cytotoxic stress, clomipramine causes disruptions to autophagic flow and significantly reduces the ability of tumorigenic cells to survive [3]. In primary neuronal cultures, clomipramine decreases autophagy. Neuronal autophagy pathway in primary cultured cells is negatively regulated by clomipramine (1 and 5 µM) [3].
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|---|---|
| ln Vivo |
Mice exposed to 5–20 mg/kg of clomipramine intraperitoneally develop significant hyperglycemia. Because clomipramine blocks 5-HT2B and/or 5-HT2C receptors, it causes hyperglycemia in mice by encouraging the release of adrenaline. Clomipramine decreased immobility in mice during the forced swim test, which is used as a behavioral model for antidepressants. Additionally, clomipramine prevents mice used as animal models for obsessive-compulsive disorder from burying marbles [1]. In mouse tissues, clomipramine (20 mg/kg) decreases autophagic flow [3].
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| Cell Assay |
Western Blot Analysis[3]
Cell Types: Primary Cortical Neurons Tested Concentrations: 1 and 5 µM Incubation Duration: 12, 24 and 48 hrs (hours) Experimental Results: Enhanced LC3-I to LC3-II in a concentration-dependent manner at all analyzed time points transformation. |
| Animal Protocol |
Animal/Disease Models: C57BL/6 J mice (6 weeks old, 22 to 25 g) [3]
Doses: 20 mg/kg Route of Administration: intraperitoneal (ip) injection for 21 days Experimental Results: LC3-II and p62 were Dramatically increased in the liver High clomipramine-treated mice were compared with vehicle-treated mice. |
| References |
[1]. Yumi Sugimoto, et al. The tricyclic antidepressant Clomipramine increases plasma glucose levels of mice. J Pharmacol Sci. 2003 Sep;93(1):74-9.
[2]. Mushtaq Ahmed, et al. Comparative study of the inhibitory effect of antidepressants on cholinesterase activity in Bungarus sindanus (krait) venom, human serum and rat striatum. J Enzyme Inhib Med Chem. 2008 Dec;23(6):912-7. [3]. Federica Cavaliere,The tricyclic antidepressant Clomipramine inhibits neuronal autophagic flux. Sci Rep. 2019 Mar 19;9(1):4881. |
| Molecular Formula |
C19H23N2CL
|
|---|---|
| Molecular Weight |
314.85232
|
| CAS # |
303-49-1
|
| Related CAS # |
Clomipramine hydrochloride;17321-77-6;Clomipramine-d3;136765-29-2
|
| SMILES |
CN(C)CCCN1C2=CC=CC=C2CCC3=C1C=C(C=C3)Cl
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1761 mL | 15.8806 mL | 31.7612 mL | |
| 5 mM | 0.6352 mL | 3.1761 mL | 6.3522 mL | |
| 10 mM | 0.3176 mL | 1.5881 mL | 3.1761 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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