| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
Clenbuterol targets the β2-adrenergic receptor. It acts as an agonist, binding to beta-2 adrenergic receptors on smooth muscle cells, leading to the activation of adenylate cyclase and subsequent increase in intracellular levels of cyclic AMP. It activates the protein kinase A pathway, regulating gene expression and protein synthesis. It has an EC50 of 31.9 nM.
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| ln Vitro |
In rat primary adipocytes, clenbuterol enhances lipolysis in contrast to control. In cultures with 0.1 or 1 μM clenbuterol, respectively, free glycerol release into the culture media is 158% and 190% of control values[1].
In vitro, Clenbuterol increases lipolysis in rat primary adipocytes, with free glycerol release reaching 158% and 190% of control values at 0.1 and 1 μM, respectively. It inhibits the release of TNF-α and IL-1β induced by lipopolysaccharide (LPS). It is used in cell-based assays to study β2-adrenergic receptor signaling and its effects on cellular metabolism. |
| ln Vivo |
Seven days after the middle cerebral artery is permanently blocked, the cortical infarct volume of male Long-Evans rats is assessed using clenbuterol hydrochloride (0.01-0.5 mg/kg)[2].
In vivo, Clenbuterol (0.01-0.5 mg/kg; i.p.) reduces the cortical infarct volume in Long-Evans rats as measured 7 days after permanent occlusion of the middle cerebral artery. It exhibits neuroprotective and anti-inflammatory activities. It also decreases adipose cell size and increases muscle fiber size. |
| Enzyme Assay |
Clenbuterol is evaluated in cell-free receptor binding assays to determine its affinity for β2-adrenergic receptors. Radioligand binding displacement assays are performed using membrane preparations expressing β2-AR. Competition binding experiments are conducted with increasing concentrations of Clenbuterol and a fixed concentration of a labeled reference ligand.
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| Cell Assay |
MTS assay is performed to determine the number of viable cells in culture. Cells are treated with 0 (vehicle only), 0.1, or 1 μM Clenbuterol for 6, 12, 24, or 48 h. The absorbance is measured at 490 nm in a plate reader determine the formazan concentration, which is proportional to the number of living cells in culture[1]. |
| Animal Protocol |
Clenbuterol is administered orally or intraperitoneally in animal models. In rats, it is administered via drinking water (30 mg/L) for 3 weeks. In stroke models, it is given at 0.01-0.5 mg/kg i.p.. Efficacy is assessed by measuring infarct volume, muscle mass, and behavioral outcomes.
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| ADME/Pharmacokinetics |
Clenbuterol is orally active and is absorbed from the gastrointestinal tract. It has a molecular weight of 313.65 and a molecular formula of C12H18Cl2N2O·HCl. Its CAS number is 21898-19-1. The compound is soluble in DMSO at 83.33 mg/mL. It should be stored at 4°C, sealed, and away from moisture.
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| Toxicity/Toxicokinetics |
No detailed toxicity data is available for Clenbuterol beyond its known use as a bronchodilator. It is classified as a Dangerous Good for transport. It is a β2-adrenergic agonist and is available by prescription only.
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| References |
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| Additional Infomation |
Clenbuterol hydrochloride is the monohydrochloride salt of clenbuterol. It is a bronchodilator, β-adrenergic agonist, and sympathomimetic agent. It contains clenbuterol(1+), a substituted phenylaminoethanol that has β-2-adrenergic agonist activity at very low doses. It is used to treat bronchodilation in the treatment of asthma. See also: Clenbuterol (containing the active fraction).
Clenbuterol is also known as NAB-365. It is a selective β2-adrenergic agonist with bronchodilator and muscle-building properties. It is used in research to study β2-adrenergic receptor signaling, muscle hypertrophy, and neuroprotection. The compound is not approved for human use in some countries. |
| Molecular Formula |
C12H19CL3N2O
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|---|---|
| Molecular Weight |
313.65
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| Exact Mass |
312.056
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| CAS # |
21898-19-1
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| Related CAS # |
Clenbuterol;37148-27-9
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| PubChem CID |
5702273
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| Appearance |
White to off-white solid powder
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| Density |
1.25g/cm3
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| Boiling Point |
404.9ºC at 760 mmHg
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| Melting Point |
174-175.5°C
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| Flash Point |
198.7ºC
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| LogP |
4.771
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
18
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| Complexity |
233
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
OPXKTCUYRHXSBK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H18Cl2N2O.ClH/c1-12(2,3)16-6-10(17)7-4-8(13)11(15)9(14)5-7/h4-5,10,16-17H,6,15H2,1-3H31H
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| Chemical Name |
1-(4-amino-3,5-dichlorophenyl)-2-(tert-butylamino)ethanolhydrochloride
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| Synonyms |
Clenbuterol Hydrochloride NAB-365 Planipart Clenbuterol HCl NAB365Spiropent NAB 365Clenbuterol clorhidrato
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~265.68 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1883 mL | 15.9413 mL | 31.8827 mL | |
| 5 mM | 0.6377 mL | 3.1883 mL | 6.3765 mL | |
| 10 mM | 0.3188 mL | 1.5941 mL | 3.1883 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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